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Validated All-in-One™ qPCR Primer for SERPINB3(NM_006919.2) Search again
Product ID:
HQP016581
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
HsT1196, SCC, SCCA-1, SCCA-PD, SCCA1, SSCA1, T4-A
Gene Description:
serpin family B member 3
Target Gene Accession:
NM_006919.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- concentration and the positivity rate significantly elevated with esophageal squamous cell tumor progression; differences depended on tumor size, tumor depth, lymph node status, and distant metastasis
- in the described patient groups, SCC is probably of low value for tumor diagnosis and follow-up
- interacts with specific endogenous proteinases such as cathepsins L and L2 in physiological and pathological states of squamous epithelium
- the inhibitory mechanism of SCCA1 is unique among the serpin superfamily
- transfection of SCCA1 into mammalian cells (both hepatocyte-derived and of non-hepatocyte origin) results in increased HBV binding
- Findings suggest that genetic diagnosis by RT-PCR based on SCCA mRNA expression may be clinically useful for detecting occult tumor cells in cervical lymph nodes.
- E-cadherin mediated cell-cell adhesion maintains SCCA production through PI 3-kinase in squamous cell carcinoma.
- SCCA variants are overexpressed in hepatocellular carcinoma independently of tumour etiology; a novel SCCA1 variant has been identified in one third of liver tumours
- Hepatitis B virus sequence is able to induce overexpression of SCCA at the cell surface in squamous cell carcinoma.
- Exposure of uterine cervical squamous carcinoma CaSki cells to CDDP resulted in a transient increase in SCC Ag protein and mRNA expression
- Uteroglobin suppresses SCCA gene expression associated with airway inflammation in asthma
- Single nucleotide polymorphism of SCCA2 indicated that this polymorphism may underlie the difference in promoter activity between SCCA1 and SCCA2.
- the hydrophobicity of the reactive site loop of SCCA1 may play an important role in hepatitis B virus binding to cells
- alpha-fetoprotein and SCCA yielded a correct serologic diagnosis in 90.83% of the hepatocarcinoma patients.
- squamous cell carcinoma antigen is expressed in peripheral blood of cancer patients
- SCC-Ag mRNA levels in the peripheral blood are the best predictive factor for recurrence in patients with esophageal squamous cell carcinoma who undergo curative resection.
- The expression pattern of SCCA1 in esophageal dysplasia and squamous cell carcinmoma was studied.
- Squamous cellular carcinoma immunocomplexed is increased in scleroderma patients with lung fibrosis.
- Serum levels of MMP-9, CEA and SCC-Ag in esophageal cancer (EC) patients were statistically higher than in controls
- Blood levels of IgG autoantibodies that bind to squamous cell carcinoma antigen proteins were significantly higher in psoriasis than healthy controls.
- Activated STAT3 is a required part of the continuous activation of B3/B4 genes, which protects tumor cells from dying.
- Elevated squamous cell carcinoma antigen is associated with hepatocellular carcinoma.