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Validated All-in-One™ qPCR Primer for ROBO1(NM_002941.3) Search again
Product ID:
HQP016368
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CPHD8, DUTT1, NORS, NYS8, SAX3
Gene Description:
roundabout guidance receptor 1
Target Gene Accession:
NM_002941.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline.
Gene References into function
- Tumour specific promoter region methylation of ROBO1 in human cancers
- a slight disturbance in neuronal axon crossing across the midline between brain hemispheres, dendrite guidance, or another function of ROBO1 may manifest as a specific reading disability in humans
- Both medulloblastoma and glioma tumors express Robo1.
- Expression of Robo1 is mainly in tumor cells, whereas Robo4 is primarily in endothelial cells of tumor vessels. Therefore, the Robo proteins provide potential target structures for the anti-tumorigenic and anti-angiogenic therapy of colorectal carcinoma.
- The ROBO1 is overexpressed in hepatocellular carcinoma and shed into serum in humans. These observations suggest that ROBO1 is a potential new serologic marker for hepatocellular carcinoma and may represent a new therapeutic target.
- heparin/HS is an integral component of the minimal Slit-Robo signaling complex and serves to stabilize the relatively weak Slit-Robo interaction
- Dutt1 is silenced by promoter hypermethylation in lung cancer
- The minimal Robo1-binding region required for Slit activation has been mapped to the N-terminal immunoglobulin (Ig)1-2 domains.
- the crystal structures of the first two Ig domains of Robo1 (Ig1-2), and the minimal complex between these proteins (Slit2 D2-Robo1 Ig1).
- a chemorepulsive effect mediated by interaction of Slit2 and Robo1 participates in glioma cell guidance in the brain.
- mRNA expression of ROBO1 in lymphocytes was significantly reduced in the autistic group
- Robo1 is essential for Robo4-mediated filopodia induction
- Inactivation of ROBO1/DUTT1 could be used as molecular signature for early detection and prognosis of the head and neck cancer.