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Validated All-in-One™ qPCR Primer for RNASEL(NM_021133.3) Search again
Product ID:
HQP016303
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
PRCA1, RNS4
Gene Description:
ribonuclease L
Target Gene Accession:
NM_021133.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele. [provided by RefSeq].
Gene References into function
- Germline alterations of the RNASEL gene, a candidate HPC1 gene at 1q25, in patients and families with prostate cancer.
- Analysis of the RNASEL gene in familial and sporadic prostate cancer. A total of six variants were identified, including one intronic and five exonic changes (three missense and two silent alterations).
- Structural and functional features of the 37-kDa 2-5A-dependent RNase L in chronic fatigue syndrome
- physical performance and prediction of 2-5A synthetase antiviral pathway activity in patients with chronic fatigue syndrome.
- in rRNA and small nucleolar RNA maturation, not in telomere elongation inhibition
- A novel founder mutation in the RNASEL gene, 471delAAAG, is associated with prostate cancer in Ashkenazi Jews.
- The variant Arg462Gln has 3X less enzymatic activity than the wildtype and is significantly associated with prostate cancer risk (P=0.007).
- Author reviews the tumor suppressor role of RNase L, proposing that it functions in counteracting prostate cancer by virtue of its ability to degrade RNA, thus initiating a cellular stress response that leads to apoptosis.
- A genome-wide scan of high risk prostate cancer families in North America has demonstrated linkage of a particular marker to chromosme 1q(HPC1).
- polymorphic changes within the RNASEL gene may be associated with familial prostate cancer risk
- JNK and RNase L function in an integrated signaling pathway during the IFN response that leads to elimination of virus-infected cells through apoptosis
- RNASEL may function as a tumor suppressor in prostate cancer.
- does not constitute a major cell defence mechanism against the varicella-zoster virus infection
- Studies report an association between the RNASEL G1385A variant and prostate cancer risk; this variant does not appear to be implicated in the development of breast cancer.
- RNASEL does not have a major role in prostate cancer etiology
- These results indicated that the ankyrin-repeat domain of RNase L constricts its structure by binding of 2-5A. This observation suggests a revised model of the 2-5A-induced activation of RNase L.
- Human translation termination factor eRF3/GSPT1 is an interacting partner of RNase L.
- results suggest that RNASEL variants Glu265X and Arg462Gln may contribute to the tumorigenesis of sporadic and familial pancreatic cancer
- Single nucleotide polymorphisms associated with hereditary prostate cancer.
- androgen receptor and the interferon-activated RNase L interact with each other in a ligand-dependent manner
- 2'-5'-linked oligoadenylate activation of RNase L produces a remarkable stimulation of transcription (>/=20-fold) for genes that suppress virus replication and prostate cancer.
- characterization of 2',5'-linked oligoadenylate binding determinant of human RNase L
- single nucleotide polymorphisms (SNPs) identified in RNASEL exons in hospitalized patients with West Nile Virus infection
- RNASEL genetic alterations does not support a significant role in prostate cancer predisposition in Israeli Ashkenazi Jews.
- Data suggest that the primary role of double-stranded RNA binding by the NS1A protein in virus-infected cells is to sequester dsRNA away from RNAse L.
- Gene is associated with inherited prostatic cancer. [review]
- A silent polymorphism in the RNASEL gene occurs more prevalently in high-risk Ashkenazi breast/ovarian cancer patients without a BRCA1/2 mutation.
- Compared with the genotype Asp/Asp, the Glu variant at the Asp541Glu polymorphism increases prostate cancer risk by <2-fold in Caucasians, regardless of family history of the disease.
- Report of the crystal structure of the N-terminal ankyrin repeat domain of human RNase L complexed with an activator molecule containing a 5'-phosphorylated 2',5'-linked oligoadenylate, [(pp)p(A2'p5')(n)A].
- the IFN-inducible RNase L may play an important role during stress-response through RNA-degradation and apoptosis
- study indicated only p53 & RNASEL genotypes had significant influence on age of onset of Lynch syndrome in an additive mode of inheritance & that effects of both variants are purely additive supporting the notion
- Positive association between higher trans-fatty acid consumption and prostate cancer may be modified by the functional RNASEL variant R462Q.
- HuR-dependent regulation of RNase-L enhances its antiviral activity, demonstrating the functional significance of this regulation
- the effects of oligo A synthetase/RNase on the replication cycle of parainfluenza virus type 5
- RNase L could have a role in cancer biology and evidence of a tumor suppressor function of RNase L has emerged from studies on the genetics of hereditary prostate cancer [review]
- results suggest that the RNASEL Gln/Gln genotype does not play an important role in the etiology of prostate cancer in the general population
- RNASEL has a role as a predisposition gene for prostate cancer in African Americans and Hispanic Caucasians
- common variation in the putative prostate cancer susceptibility gene, RNASEL, or its inhibitor does not contribute significantly to prostate cancer risk in Tobago Afro-Caribbean population
- This prospective study suggests that prostate cancer in patients with the R462Q allelic variant of the HPC1/RNASEL gene is not associated with more aggressive clinical or pathological features in radical prostatectomy specimens.
- Antagonistic coevolution may have occurred between a specific host locus involved in immune defense (RNASEL) and a viral pathogen.
- Our results do not support a role for RNASEL, or MSR1 mutations in advanced Asian-Indian PC.
- discovered an increased risk, a heterozygous advantage and thereby a protective effect linked to the RNASEL SNP rs3738579
- HPC2/ELAC2 and RNASEL may play a role, however minor, in prostate cancer risk among African American men.
- A putative loop E motif and an H-H kissing loop interaction are key features of the group C enterovirus RNA associated with the inhibition of RNase L.