|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for RBP4(NM_006744.3) Search again
Product ID:
HQP016198
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
MCOPCB10, RDCCAS
Gene Description:
retinol binding protein 4
Target Gene Accession:
NM_006744.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Summary
This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues.
Gene References into function
- Genetic variation in RBP4 is associated with amyloid polyneuropathy
- generated models for the ensemble of conformers populated within this molten globule state; conformer changes give an opening of the retinol-binding site
- Our findings point to profound differences between rodents and humans in the regulation of adipose or circulating RBP4 and challenge the notion that glucose uptake by adipocytes has a dominant role in the regulation of RBP4.
- the rare alleles of four SNPs were associated with increased risk of diabetes (-803, G > A, P = 0.0054; +5169, C > T, P = 0.0025; +6969, G > C, P = 0.0015; +7542, T > del, P = 0.0015)
- Plasma RBP4 concentrations were found to be elevated in subjects with impaired glucose tolerance or type 2 diabetes and to be related to various clinical parameters known to be associated with insulin resistance
- Reductions in circulating RBP-4 may contribute to improved insulin resistance in morbidly obese subjects after weight loss.
- a haplotype of 8 common SNPs in Caucasians was significantly increased in type 2 diabetics compared with controls
- Circulating RBP4 is not associated with the amount of fat in liver, skin, viscera or muscles.
- there is a relationship between RBP4, insulin sensitivity, and percent trunk fat in individuals who may not have features of insulin resistance
- RBP4 is not a relevant systemic factor in the pathogenesis of insulin resistance in liver cirrhosis.
- Severe calorie restriction promotes a reduction in adipose tissue and plasma levels of RBP4.
- up-regulation of RBP4 mRNA in subcutaneous and omentum adipose tissue as well as isolated subcutaneous adipocytes of polycystic ovary syndrome women
- Serum retinol binding protein 4 levels are significantly increased in polycystic ovary syndrome women and associated with insulin resistance.
- RBP4 may contribute to the development of insulin resistance along with other adipokines
- Plasma RBP4 levels in type 2 diabetic patients are affected by incipient nephropathy.
- RBP4 may be released from diabetic adipocytes and act locally to inhibit phosphorylation of IRS1 at serine (307), a phosphorylation site that may integrate nutrient sensing with insulin signaling
- RBP4 gene expression in humans is associated with inflammatory markers, but not with insulin resistance
- Serum retinol-binding protein is more highly expressed in visceral than in subcutaneous adipose tissue and is a marker of intra-abdominal fat mass
- Markers of glucose and lipid metabolism were not independently related to serum RBP-4 in control subjects or chronic hemodialyis patients.
- RBP4 does not seem to be a valuable marker for identification of the metabolic syndrome or insulin resistance in male patients with type 2 diabetes or coronary artery diseae.
- Contribution of RBP4 from adipocytes to circulating adipokine levels should be evaluated in diabetic and obese patienets.
- In obese men with metabolic syndrome, weight loss with a low-fat diet decreases the plasma LDL apoB-100 and HDL lipoprotein kinetics, involving changes in RBP4 levels.
- Role of RBP4 genetic variation in susceptibility to type 2 diabetes and insulin resistance, possibly through an effect on RBP4 expression.
- RBP4 may represent a link between visceral obesity and cardiovascular disease
- Retinol binding protein 4 may contribute to the pathogenesis of nonalcoholic fatty liver disease in type 2 diabetics.
- Serum RBP4 and TTR showed no differences between controls/type 1 diabetic children.
- Report retinol-binding protein 4 expression in visceral and subcutaneous fat in human obesity.
- The associations of RBP4 with insulin sensitivity, percent trunk fat, and lipid levels are influenced by age
- In fibroboasts, STRA6 transports retinol bidirectionally in an RBP4 dependent manner.
- RBP4 level could be used as an index of cardiovascular disease risk in subclinical hypothyroidism.
- RBP4 is not a useful marker of insulin resistance in polycystic ovary syndrome but may reflect other metabolic features of this condition.
- RBP4 levels were related to weight status and insulin resistance in both cross-sectional and longitudinal analyses.
- Data show that increase in RBP4 from early to late pregnancy, associated with a decline in insulin sensitivity, potentially indicates interactions with glucose metabolism.
- Neither retinol nor RBP-4 were associated with peak bone mineral density in young men
- Relationship between circulating RBP4 and iron stores, both cross-sectional and after iron depletion, and in vitro findings suggest that iron could play a role in the RBP4-insulin resistance relationship.
- RBP4 measured by two different techniques is not elevated, but the RBP4:retinol molar ratio is higher and correlates with fasting blood glucose in women with gestational diabetes
- Although associated with visceral fat, serum RBP4 and adiponectin levels do not play important, fat-mass-independent primary roles in the development of PCOS.
- The severity of glucose intolerance in women with previous gestational diabetes mellitus is associated with high RBP4 and low adiponectin concentrations.
- Weight reduction, especially the loss of abdominal visceral fat, lowers serum retinol binding protein-4(RBP4) concentrations in nondiabetic subjects indicating that RBP4 may be involved in improvement of insulin resistance and metabolic syndrome
- Homozygosity for the RBP4 -803A allele is associated with increased risk of type 2 diabetes in the Rotterdam population.
- In nondiabetic, nonobese patients with genotype 1 chronic hepatitis C, serum RBP4 levels might be expression of virus-linked pathway to steatosis, largely unrelated to insulin resistance.
- This is the first report of a long-term longitudinal study on RBP and the major finding is that subjects who developed insulin resistance showed no change in plasma RBP.
- Investigations into the role of RBP in obesity and insulin resistance should include retinol to facilitate the measurement of apo-RBP and retinol:RBP.
- There is a marked decrease of RBP-4 levels after bariatric surgery, which correlates with reduction in visceral-fat mass. The extent of changes in RBP-4 levels differs according to the severity of the metabolic syndrome.
- The occurrence of RBP4 isoforms is not influenced by liver function but seems to be strongly related to kidney function and may therefore be important in investigating kidney function and related disorders.
- Cohort study provides evidence that RBP4 may be a mechanism through which obesity influences insulin resistance and hypertriglyceridemia in overweight postpubertal black youth.
- Human data showing elevated RBP4 levels in type 2 diabetic patients may be the result of moderate renal insufficiency rather than support for the suggestion that RBP4 links obesity to type 2 diabetes.
- Measurement of serum RBP4 does not provide added value for predicting CAD risk beyond traditional risk factors.
- Impaired renal clearance of diabetic nephropathy affects RBP4 and indirectly supports the hypothesized link among metabolic syndrome, uric acid and insulin resistance
- RBP4 may be linked to IR and lipid metabolism, at least in the elderly.
- conjugated linoleic acid did not induce changes in RBP4 concentrations in abdominally obese middle-aged men despite marked induced insulin resistance
- The protein-protein interactions between transthyretin and plasma retinol-binding protein were analysed by structural (X-ray crystallography) and genetic (amino acid substitutions) methods.
- Hemodyalysis and renal transplataion patients had elevated RBP4, apo-RBP4, RPB4-L levels.
- our findings do not confirm a link between insulin resistance, neither with RBP4 nor with free plasma RBP4 levels.
- RBP4 did not correlate to clinical and biochemical measures of pregnancy outcome, renal function, glucose, and lipid metabolism in preeclampsia.