|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for TRPV4(NM_021625.4) Search again
Product ID:
HQP016163
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
BCYM3, CMT2C, HMSN2C, OTRPC4, SMAL, SPSMA, SSQTL1, TRP12, VRL2, VROAC
Gene Description:
transient receptor potential cation channel subfamily V member 4
Target Gene Accession:
NM_021625.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Summary
This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Gene References into function
- Activation of TRPV4 channels (hVRL-2/mTRP12) by phorbol derivatives.
- TRPV4 is a channel-forming protein that response to mutationa in its putative pore region with change in premeability and block.
- TRPV4 is a functional temperature-sensing channel in native endothelium, that is likely involved in temperature-dependent Ca(2+) signaling
- TRPV4 activity is tightly controlled by intracellular Ca2+. Ca2+ entry increases both the rate and extent of channel activation by a calmodulin-dependent mechanism.
- functional TRPV4 is expressed in human airway smooth muscle cells and may act as an osmolarity sensor in the airway.
- results suggest that defective regulatory volume decrease (RVD) in cystic fibrosis airway epithelia might be caused by the absence of transient receptor potential cationic channel TRPV4-mediated Calcium(2+) signal
- TRPV4 channel is involved in the coupling of fluid viscosity changes to epithelial ciliary activity.
- Ankyrin domains are necessary for oligomerization of TRPV4; and lack of TRPV4 oligomerization determines its accumulation in the endoplasmic reticulum.
- there is a single high-probability N-linked glycosylation site in TRP4 that faces the extracellular milieu and is phylogenetically conserved
- data indicate that transient receptor potential channel 4(TRPV4) is functionally regulated by WNK family kinases 1 and 4 at the level of cell surface expression
- TRPV4 participates in hypotonic reduction of AQP5.
- studies with suggest a role for the channel in the regulation of body osmolarity, mechanosensation, temperature sensing, vascular regulation and, possibly, hearing--{REVIEW}
- TRPM3 may have diverse cellular functions depending on the expression of a particular variant while TRPV4 plays a central role in epithelial homoeostasis by modulating epithelial barrier function [review]
- this study shows for the first time that TRPV4 and actin intimately associate within living cells.
- accumulation of TRPV1 and TRPV3 in peripheral nerves after injury, in spared axons, matches our previously reported changes in avulsed DRG.
- OS-9 regulates the secretory transport of TRPV4 and appears to protect TRPV4 subunits from the precocious ubiquitination and ER-associated degradation.
- PACSIN 3 acts as an auxiliary protein of TRPV4 channel that not only affects the channel's subcellular localization but also modulates its function in a stimulus-specific manner.
- altered expression of the channel in CRS and presumed expression of TRPV4 in secretory cells of the mucosa indicate a potential role in mucus homeostasis and CRS pathogenesis.
- low-level shear stress enhances epithelial barrier function, an effect that requires serial activation of the transient receptor potential vanilloid (TRPV) 4 and L-type voltage-gated calcium channel (VGCC) and an increase in intracellular calcium.
- These data indicate that the C-terminus of TRPV4 is required for oligomerization, which takes place in the endoplasmic reticulum and precedes plasma membrane trafficking.
- Data indicate that TRPV4 contributes to mechanically evoked visceral pain in inflammatory bowel disease.
- There is TRPV4 functional expression in human corneal epithelial cells.
- Study shows that inositol trisphosphate (IP3), sensitizes TRPV4 to epoxyeicosatrienoic acid but not to other TRPV4 physiological stimuli such as warm temperature, an effect that requires a functional IP3 receptor.
- play a role in pathophysiology of neurogenic inflammation.
- In two families with an autosomal dominant form of brachyolmia study identified point mutations in TRPV4 that encoded R616Q and V620I substitutions, respectively.
- In cultured human endothelial cells, exposure to fluid shear stress induced the translocation of the TRPV4 channel from a perinuclear localization to the cell membrane.
- Messenger RNA transcripts of TRPVs 1 through 4 are detected for the first time in human pulmonary artery smooth muscle cells.
- Report TRPV4-like non-selective cation currents in cultured aortic myocytes.
- relative gene expression ofTRPV1-4 in leukocytes is: TRPV3 < TRPV4, TRPV1 and TRPV2; leukocytes in hyposensitive subjects showed up-regulation of TRPV1, which was almost doubly expressed