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Validated All-in-One™ qPCR Primer for PTPRC(NM_002838.4) Search again
Product ID:
HQP015908
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
B220, CD45, CD45R, GP180, IMD105, L-CA, LCA, LY5, T200
Gene Description:
protein tyrosine phosphatase receptor type C
Target Gene Accession:
NM_002838.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Summary
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq].
Gene References into function
- role of CD45RO in the persistent HTLV-1 infection in vivo
- CD45 variant (C77G, exon 4) does not confer susceptibility to either IDDM or Graves' disease
- we examined the frequency of the C77G allele in African and Asian populations from countries with high or low prevalence of HIV infection; Here we report that the variant CD45 C77G allele is absent in African populations
- Requirements of src family kinase activity associated with CD45 for myeloma cell proliferation by interleukin-6
- SKAP55 coupled with CD45 positively regulates T-cell receptor-mediated gene transcription.
- analysis of CD45 isoform expression during T-cell development and selection in the human thymus
- CD45 controls interleukin-4-mediated IgE class switch recombination in human B cells
- Receptor tyrosine phosphatase, CD45 binds galectin-1 but does not mediate its apoptotic signal in Jurkat cells
- Role of high and low molecular weight isoforms of CD45 in the function of naive and memory T lymphocytes. Review.
- a mutation in PTPRC interferes with splicing and alters the structure of the CD45 molecule
- Biological functions of membrane expressed CD45 isoforms on PMNs were studied. Cross-linking of CD45 isoforms by specific MAbs stimulated different PMN activities by differential suppression on protein tyrosine phosphorylation and p56lck.
- Role of the C-->G mutation in position 77 of exon 4 of the protein tyrosine phosphatase receptor-type C (PTPRC) gene, coding for the CD45 molecule, for the development of multiple sclerosis (MS) in Italy.
- The spectrin-ankyrin skeleton controls CD45 surface display and interleukin-2 production.
- interactions between RPTP-domain1s and RPTP-domain 2s are a common but specific mechanism that is likely to be regulated- domain2s and the wedge structures are crucial determinants of binding specificity, thus regulating cross-talk between RPTPs
- reaches the cell surface via Golgi-dependent and -independent pathways
- CD45 may play a pivotal role in erythropoiesis. CD45 tyrosine phosphatase inhibits erythroid differentiation of umbilical cord blood CD34+ cells associated with selective inactivation of Lyn.
- difference in sensitivity to stress stimuli and IL6-induced cell growth between CD45+ and CD45- multiple myeloma cells
- CD45 is regulated by ST6Gal I sialyltransferase in a process that leads to T cell death
- CD45 differentially regulates CXCR4-mediated chemotactic activity and MAPK activation by modulating the activities of focal adhesion components
- CD45 mediates its T cell inhibitory activity, causing PP14 to elevate TCR activation thresholds and thereby down-regulate T cell activation
- CD45 via its Janus kinase phosphatase activity is able to suppress IL-4-dependent activation-induced expression of cytidine deaminase in primary B cells.
- data identify CD45 as a gene associated with autoimmune hepatitis, and further substantiates the hypothesis that CD45 represents a modifier gene of human autoimmunity
- Association of a PTPRC exon A mutation in systemic sclerosis.
- CD45 is excluded from the inhibitory, i.e., noncytolytic, but not the activating, NK cell immune synapse, where its redistribution away from intercellular contact may favor inhibitory effector functions.
- The loss of CD45 activity in lymphocytes from the elderly may underlie poor T cell function associated with ageing.
- a polymorphism in exon 6 (A138G) of the gene encoding CD45 that interferes with alternative splicing
- prevalence of a functional mutation in the CD45 gene and distribution of memory and naive T cells in myasthenia gravis
- None of four newly identified nucleotide substitutions in the CD45 gene, namely C77T (Pro59Pro) in exon 4, G69C (Asp121His) in exon 5, T127A (Ile187Asn) and A138G (Thr191Ala) in exon 6, is significantly associated with multiple sclerosis.
- identification of CD45RO+ T-cells in the fetus
- strong physical linkage of CD26 with CD45RA outside lipid rafts may be responsible for the attenuation of T-cell activation signaling through CD26
- Review. Myeloma cells expressing CD45 antigens which contain the activation of src family protein-tyrosine kinases independent of IL-6 stimulation proliferate in response to IL-6, but the proliferation of CD45- cells lacking src family PTKs is not.
- CD45-D2 has a role in binding substrate and binds the SD10 region in Lck, which is a novel site involved in substrate recognition
- concluded that CD4+ T lymphocytes from neonates with fetal distress show a transient decrease in the CD45RA expression without an increase in the CD45RO expression
- Results describe a sequence element that is both the primary determinant of CD45 variable exon exclusion following T cell stimulation by PMA and is sufficient to confer activation-induced skipping of a heterologous exon.
- IL-6-dependent multiple myeloma cells require CD45 to initiate IL-6 signaling and to maintain Lyn kinase activity, both of which are essential for cell proliferation and cell adhesion.
- Antiretroviral therapy with the anti-CD45RO immunotoxin might reduce the HIV latent reservoir without seriously compromising CD8+ T cell memory responses.
- CD45 splicing abnormalities might be associated with HIV infection
- Coexpression of CD45RA and CD45RO molecules indicates activation of maternal CD4(+) and CD8(+) lymphocytes
- masking of the alpha2-6-linked sialic acid binding site of CD22 may be mediated by secondary interactions with Sias on CD45 and sIgM
- CD45 plays a significant role in nuclear apoptosis by the regulation of the chloride channels responsible for ionic homeostasis of the cell.
- The A138G polymorphism is the cause of altered CD45 isoform expression, promoting splicing towards low molecular weight CD45 isoforms.
- C to G transversion at pos. 77 does not seem to be associated with susceptibility for Type 1 diabetes.
- the 77C-->G PTPRC polymorphism is present and preferentially transmitted in a small subgroup (<5%) of multiple sclerosis families
- novel isoform that results from the alternative splicing of a previously undiscovered exon between the constitutively spliced exon 3 and the alternatively spliced exon A
- a rapid translocation of CD45RO to lipid rafts may be responsible for IL-6-induced proliferation, and the change from CD45RA to CD45RO confers the ability to respond to IL-6 in human myeloma cells.
- Results describe the crystal structure of the cytoplasmic D1D2 segment of human CD45 in native and phosphotyrosyl peptide-bound forms.
- Data suggest that, in addition to their established roles in the initiation of T cell receptor (TCR) signaling, CD45 and Lck may also influence the type of TCR signal generated.
- May involve T cell hyperreactivity and increaese the risk of graft rejection.
- CD45 isoform expression is controlled by the combined activity of basal and inducible splicing-regulatory elements in each of the variable exons
- CD45 expression accompanied by the increased VDAC1 expression sensitizes myeloma cells to the various extracellular stimuli that trigger apoptosis via the mitochondrial pathways
- placental protein 14 promotes CD45 dimerization and clustering, a phenomenon that may regulate CD45 activity
- CD4+CD25bright T cells, predominantly within the CD45RO+ activated/memory subset in adults and the CD45RA+ naive T-cell subset in infants, are considered to be the equivalent subset.
- compared T cell reactivity in heterozygous carriers of the mutation (healthy individuals and multiple sclerosis patients) and wild-type controls
- results implicate CD45, Cbl, Cbl-b, src kinases and potentially other associated proteins as mediators of SDF-1alpha/CXCL12-induced cell migration of Jurkat T cells
- Changes in CD45 isoform expression can alter immune function in human C77G variants and CD45 transgenic mice. The C77G allele may influence the outcome of hepatitis C virus infection.
- tyrosine phosphatase CD45 mediates HIV-1 gp120-mediated apoptosis of T cells
- The 138G allele is widely distributed in the Far East and India, indicating that it may have a significant effect on disease burden in a large part of the human population.
- CD45RA+ CCR7- CD8+ T cells are resting memory cells that, upon antigenic stimulation and then proliferate, lose CD45RA, and transiently acquire CCR7.
- CD4+ CD45RO+ Foxp3+ CD25hi T lymphocytes are highly proliferative, with a doubling time of 8 days, compared with memory CD4+ CD45RO+ Foxp3- CD25- (24 days).
- Interactions of macrophage galactose C-type lectin (MGL) with CD45 on effector T cells negatively regulate T cell receptor-mediated signaling and T cell-dependent cytokine responses.
- in vitro infection system of phytohemagglutinin-stimulated lymphocytes provides a model for the study of infected CD45RO(+) lymphocytes but not CD45RA(+) lymphocytes
- Increased levels of cellular adhesion molecules in patients with coronary artery ectasia may be an indicator of endothelial activation and inflammation and are likely to be in the causal pathway leading to coronary artery ectasia.
- we propose a new, immunoregulatory model for Jacalin, wherein glycosylation-dependent interactions of Jacalin with CD45 on T cells elevate TCR-mediated signaling, which thereby up-regulate T cell activation thresholds and Th1/Th2 cytokine secretion.
- Blood and bone marrow cells in colorectal cancer depleted of CD45 do not show an enrichment of cytokeratin 20 as shown by PCR.
- These results are the first to demonstrate that CD45 is important in promoting cell survival by modulating integrin-mediated FAK/ERK signaling in Jurkat T cells.
- CD4(+)CD25(+)CD45RO(+)IL-7Ralpha(high) cell population contained allospecific CD4 T cells and secreted effector cytokines.
- CD45RO-associated increase in somatic mutations is attributable to cycling of cells that may have recently undergone B cell receptor-mediated selection, or are potentially in developmental transition between centrocyte and centroblast stages.
- Circulating transgenic CD45-expressing cells play an important role in regenerating the uterine epithelium of novel transgenic mice.
- We found four CD45 expression patterns: very low in typical CLL; relatively low in MCL; intermediate intensity in MZL, LPL, and FL; very high expression in HCL.
- indicate that PEGylation of radiolabeled anti-CD45 antibody may be a useful and desirable means of extending blood half-life and enhancing efficacy
- Using a pharmacological Erk inhibitor, we show that the Erk/MAPK pathway is involved in IL-6-induced clonogenicity of CD45+, but not CD45- myeloma cell lines.
- Microdomain-localized CD45 inactivates Lck and inhibits TCR signaling at the early immune synapse
- CD45 expression plays a key role in internucleosomal DNA fragmentation and chromatin condensation processes during apoptosis.
- Targeting human CD34+ hematopoietic stem cells with anti-CD45 x anti-myosin light-chain bispecific antibody preserves cardiac function in myocardial infarction.
- findings show a substantial linkage disequilibrium across the CD45 gene; most 138G alleles are present on only one haplotype, which is associated with Graves' disease, supporting previous data that A138G is a functionally important CD45 polymorphism
- T-cell proliferation induced by dendritic cells or CD3 antibodies was inhibited in the presence of cytoplasmic tail of CD45
- The results do not confirm PTPRC C77G as a general and independent risk factor for development of systemic sclerosis.
- identification of heterogeneous ribonucleoprotein L-like (hnRNPLL) as a critical inducible regulator of CD45 alternative splicing
- no evidence for the contribution of the 77C>G transversion in susceptibility to inflammatory bowel disease
- These data establish that hnRNP LL plays a critical and unique role in the signal-induced regulation of CD45 and demonstrate the utility of cell-based screens for the identification of novel splicing regulatory factors.
- activated CD45RB translocates to lipid rafts and interferes with lipid raft localization
- Data show that the C77G and C772T variations within the human protein tyrosine phosphatase receptor type C gene is associated with multiple sclerosis in an Australian population.