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Validated All-in-One™ qPCR Primer for RAD18(NM_020165.3) Search again
Product ID:
HQP015190
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
RNF73
Gene Description:
RAD18 E3 ubiquitin protein ligase
Target Gene Accession:
NM_020165.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is highly similar to S.
Gene References into function
- hRad18 is a novel interacting partner of HIV-1 integrase; role for post-replication/translesion DNA repair in the retroviral integration process.
- action at DNA replication forks
- Together, our results suggest that MMS-induced accumulation of hRad18 protein at stalled forks involves protein phosphorylation which may be performed by S-phase checkpoint kinases.
- the amount of Rad18 in the nucleus is regulated differentially by mono- and polyubiquitination
- We found that the RAD18 transcript is expressed ubiquitously in various tissues and very highly in the testis in mammals.
- Association of Polkappa with PCNA is regulated by Rad18-mediated PCNA ubiquitination.
- Rad18 is a polypeptide associated with Poleta. The study results suggest that arrested replication forks strengthen interactions among Poleta, Rad18/Rad6 and Rev1, consistent with the requirement for effective TLS by Poleta at sites of DNA lesions.
- RAD18-/- cells were defective in single-strand break repair, but functional for double-strand break repair.
- Higher-eukaryotic cells separately employ PARP1 and Rad18 to suppress the toxic effects of NHEJ during the HR reaction at stalled replication forks.
- WRN functions in a RAD18-dependent damage avoidance pathway.
- RAD18 Arg302Gln polymorphism is associated with non-small-cell lung cancer
- Single nucleotide polymorphism in the RAD18 gene is associated with colorectal cancer
- RAD18 signals DNA polymerase IOTA to stalled replication forks in cells entering S phase with DNA damage.
- The SAP domain of RAD18 (residues 248-282) is crucial for binding of RAD18 complexed with RAD6B to DNA substrates.
- A model in which RAD18 within damage-induced nuclear foci is immobilized and is required for recruitment of Y-family DNA polymerases and subsequent mutagenesis.