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Validated All-in-One™ qPCR Primer for HTRA1(NM_002775.4) Search again
Product ID:
HQP015105
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ARMD7, CADASIL2, CARASIL, HtrA, L56, ORF480, PRSS11
Gene Description:
HtrA serine peptidase 1
Target Gene Accession:
NM_002775.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7. [provided by RefSeq].
Gene References into function
- HTRA1 is a candidate tumor suppressor in ovarian cancer.
- An HtrA1 inhibitor causes accumulation of Abeta in astrocyte cell culture supernatants and colocalizes with beta-amyloid deposits in human brain samples.
- no association either with age-at-onset in AD cases or with disease risk in the case-control cohort but haplotype analysis revealed a modest association of one haplotype with AD risk (p = 0.04).
- Contributes to the destruction of extracellular matrix through both direct and indirect mechanisms; its overexpression may contribute to arthritis.
- RT-PCR analysis showed a significant reduction of HTRA1 and HTRA3 mRNA in endometrial cancer compared to normal endometrium. HTRA1 and HTRA3 protein showed a similar pattern of expression in EC tissue.
- Data suggest that serine protease HtrA1 mediates paclitaxel- and cisplatin-induced cytotoxicity and suggest that loss of HtrA1 in ovarian and gastric cancers may contribute to in vivo chemoresistance.
- it is reported that a single-nucleotide polymorphism in the promoter region of HTRA1 is a major genetic risk factor for wet age-related macular degeneration
- findings show a single-nucleotide polymorphism, rs11200638, in the promoter region of HTRA1 is the most likely causal variant for age-related macular degeneration at 10q26
- The distinct distribution of HtrA1 at the maternal-trophoblast interface suggests that HtrA1 may play a role in placental development.
- Low expression of HtrA1 is associated with lymph node metastasis of lung cancer
- highly upregulated during mucosal mast cell differentiation
- the role of HTRA1 in Age-related macular degeneration
- HTRA1 promoter polymorphism, rs11200638, is a strong candidate with a functional consequence that predisposes Japanese to develop neovascular age-related macular degeneration .
- May increase susceptibility to age-related macular degeneration (AMD) development and can participate in a potential new molecular pathway for AMD pathogenesis.
- Both the HTRA1 -625A allele and the complement factor H 402H allele are independently associated with exudative age-related macular degeneration in a Central European population.
- The LOC387715/HTRA1 variants are associated with polypoidal choroidal vasculopathy (PCV) and wet age-related macular degeneration (AMD) in the Japanese population.
- This article summarizes recent studies regarding the HtrA family of proteins, their structure, regulation and function. It also presents practical applications of this knowledge and perspective of its use in the future.
- HTRA1 mRNA expression exhibits no significant change between control and age-related macular degeneration retinas.
- the association of HTRA1 with wet age-related macular degeneration (choroidal neovascularization)
- Structural and functional analysis of the PDZ domains of human HtrA1 and HtrA3
- Although a role for PLEKHA1 could not be totally excluded, there was a four times higher age-related macular degeneration risk was associated with haplotype "A-T-A" involving "PLEKHA1-LOC387715-HTRA1" risk alleles.
- HtrA1 may regulate matrix calcification via the inhibition of BMP-2 signaling, modulating osteoblast gene expression, and/or via the degradation of specific matrix proteins
- Polymorphisms in the complement factor H gene, LOC387715, and the HTRA1 promoter are strongly associated with age-related macular degeneration.
- Independent of CFH genotype or smoking history, an individual's risk of AMD (age-related macular degeneration) could be increased or decreased, depending on their genotype or haplotype in the 10q26 region.
- There is a significant decrease in HtrA1 mRNA in malignant ovarian tumors compared to benign; HtrA1 may function as a tumor suppressor.
- The study demonstrated that both single-nucleotide polymorphisms were significantly associated with dry and wet (age-related macular degeneration) AMD.
- A high impact of the additive effect of CFH and HTRA1 in the development of exudative AMD was shown. The HTRA1-smoking additive effect found in this study further suggests the importance of this environmental risk factor in AMD.
- Possible association between occult or minimally classic choroidal neovascularization(CNV) and CFH polymorphism and between classic and predominantly classic CNV and HTRA1 polymorphism.
- Up-regulation of HtrA1 is detected in the macular lesions of age-related macular degeneration suggest that rs11200638 in HtrA1 promoter is associated with disease development.
- The present data provided an independent validation of the association of LOC387715 and HTRA1 SNPs, along with their risk estimates among Indian patients with AMD.
- expression of HPV E6/E7 proteins is associated with a post-transcriptional up-regulation of HtrA1
- The SNPs rs3753394 and rs800292 of CFH and rs11200638 of HTRA1 are significantly associated with the risk of PCV (polypoidal choroidal vasculopathy) in Chinese patients.
- A low HtrA1 score is strongly associated with a shorter overall survival in mesothelioma patients, independently from epidermal growth factor receptor expression levels.
- Both the HTRA1 and LOC387715/ARMS2 single nucleotide polymorphysms appear to contribute equally to disease risk (both geographic atrophy and choroidal neovascularization) with no evidence of interaction with CFH.
- The purpose of this study was to investigate the association of reported common single-nucleotide polymorphisms (SNPs) in CFH, LOC387715, and HTRA1 with exudative age-related macular degeneration in a northern Chinese population.
- Findings from multiple samples support an AMD genetic variant harbored within HtrA1. The risk of advanced AMD increased when the presence of risk alleles from HtrA1 was combined with either CFH risk alleles or history of smoking.
- Patients with a homozygous HTRA1 rs11200638 risk allele had larger choroidal neovascular lesions.
- The approximately 30 kDa HtrA1 form can be correlated to maternal preeclampsia while the significant down-regulation of total HtrA1 can be correlated to placental preeclampsia.
- The rs10490924 Single nucleotide polymorphisms (SNP) in LOC387715/ARMS2 and the rs11200638 SNP in HTRA1 are strongly associated with neovascular age-related macular degeneration in this Israeli population.
- In a Han Chinese population, HTRA1 polymorphisms appear to be independently and possibly additively hereditary contributors to exudative age-related macular degeneration.