Validated All-in-One™ qPCR Primer for PCDHB12(NM_018932.3) Search again

Product ID:

HQP014963 (click here to view gene annotation page)

Species:

Human

Symbol:

PCDHB12

Alias:

PCDH-BETA12

Gene Description:

protocadherin beta 12

Target Gene Accession:

NM_018932.3(click here to view gene page)

Estimated Delivery:

Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.

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Important Note:

By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.

Catalog#	Description	Note	Price (US$)
HQP014963	All-in-One™ qPCR Primer for NM_018932.3 (200 reactions)	PCDHB12	inquire
HQP006940	All-in-One™ qPCR Primer for NM_002046.6 (200 reactions)	Reference Gene GAPDH	inquire
HQP006940	All-in-One™ qPCR Primer for NM_002046.7 (200 reactions)	Reference Gene GAPDH	inquire
HQP015171	All-in-One™ qPCR Primer for NM_004048.3 (200 reactions)	Reference Gene B2M	inquire
HQP015171	All-in-One™ qPCR Primer for NM_004048.4 (200 reactions)	Reference Gene B2M	inquire
HQP016381	All-in-One™ qPCR Primer for NM_001101.5 (200 reactions)	Reference Gene ACTB	inquire

Validated result:


A	B
Figure: All-in-One™ qPCR Primer Validated result to PCDHB12. Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve.

Summary

This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific cell-cell neural connections.