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Validated All-in-One™ qPCR Primer for TRPV6(NM_018646.5) Search again
Product ID:
HQP014423
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ABP/ZF, CAT1, CATL, ECAC2, HRPTTN, HSA277909, LP6728, ZFAB
Gene Description:
transient receptor potential cation channel subfamily V member 6
Target Gene Accession:
NM_018646.5(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Calcium-permeable channels, such as TRPV6, participate in neurotransmission, muscle contraction, and exocytosis by providing calcium as an intracellular second messenger. Depending on the tissue, transcellular calcium transport may be regulated by vitamin D, parathyroid hormone (PTH; MIM 168450), or calcitonin (CALCA; MIM 114130).[supplied by OMIM].
Gene References into function
- Gene expressed in human duodenum, placenta and pancreas homologous with calcium transporters/channels in other species. Function proposed to be calcium entry channel at apical membrane.
- 1,25-Dihydroxyvitamin D3 increases mRNA expression of the CaT1 calcium channel in the Caco-2 intestinal cell line.
- to characterize ECaC2 in more detail and to compare ites properties with those of Ecac1 to obtain a better understanding of transcellular Ca2+ transport in epithelia
- regulatory component that controls activation of both CaT1 and CRAC (Ca(2+) release-activated Ca(2+) channel) channels.
- role of TRPV6 as calcium sensor in rat and human cells
- CaT1 is the channel protein that contributes to T-lymphocyte stores-operated Ca(2+) channels either alone or as a subunit in a heterogeneous channel complex.
- Our results indicate that the pattern of CaT1 and CaT2 expression correlates with the Ca2+ uptake potential along the differentiation of cultured human trophoblasts isolated from term placenta.
- the role of CaT1 in generating endogenous store-operated Ca(2+) current (I(SOC)) in the lymph node carcinoma of the prostate (LNCaP) human prostate cancer epithelial cell line
- The effects of intracellular Mg(2+) on TRPV6 are partially reminiscent of the gating mechanism of inwardly rectifying K(+) channels and may represent a novel regulatory mechanism for TRPV6 function in vivo.
- native channels responsible for ISOC are different from those for recombinant ITRPV6 and do not appear to be affected if one of their assumed subunits, TRPV6, is up- or down-regulated, suggesting a rather rigid subunit composition in vivo.
- These results suggest that all components, i.e. the store-operated calcium channel (SOCC), endoplasmic reticulum, and mitochondria, somehow contribute to the altered Ca2+ signalling in bipolar disorder.
- TRPV6 is a Ca2+-selective TRP channel lined by amino acid side chains rather than main chain carbonyls
- it is unlikely that CaT1 is a component of native CRAC channels in mast cells
- the TRPV6 channel is regulated by calcium
- the third ANK repeat of TRPV6 is required for functional subunit assembly
- the voltage dependence of TRPV6-mediated Ca(2+) influx is of physiological importance since it occurs at cytosolic Ca(2+) buffering and takes place within a physiologically relevant membrane potential range
- a strong functional link between the operation of expressed TRPC channels and endogenous SOC activity.
- PTP1B interacts with TRPV6 in vivo and plays a role in TRPV6-mediated calcium influx in HEK293 cells
- Human CAT1 is involved in erythroid hematopoiesis through its role in importing L-arginine, which appears to be essential for the differentiation of red blood cells.
- expression of TRPV6 increases the rate of Ca(2+) dependent cell proliferation which is a prerequisite for its potential role in tumor progression
- therefore suggesting that TRPC1 and/or TRPC3 proteins are responsible for the response to alpha-adrenergic stimulation but that TRPC1, TPRC3 and TRPV6 proteins, expressed alone or concomitantly, are not sufficient for SOC formation.
- Immunohistochemistry of placental villi sections evidenced presence of TRPV5-TRPV6 channels in basal and apical syncytiotrophoblast plasma membranes.
- The human transient receptor potential vanilloid type 6 distal promoter contains multiple vitamin D receptor binding sites that mediate activation by 1,25-dihydroxyvitamin D3 in intestinal cells
- Data suggest that the rate of TRPV6 protein evolution is significantly accelerated in the human lineage and that the TRPV6 haplotype defined by the derived alleles at C157R, M378V and M681T conferred a selective advantage.
- duodenal TRPV6 expression is vitamin D dependent in men, but not in older women, where expression of TRPV6 and VDR are both reduced
- We conclude that phosphorylation/dephosphorylation of tyrosines in position 161 and 162 is essential for regulation of Ca2+ influx through TRPV6 Ca2+ channels in HEK293 cells.
- there is growing evidence that transcriptional regulation of TRPV6 in certain tissues undergoing malignant transformation, such as prostate cancer, is linked to cancer progression--{REVIEW}
- TRPV5 and TRPV6 are regulated by calbindin-D(28k), klotho and BSPRY (B-box and SPRY-domain-containing protein) at different levels throughout the epithelial cell [review]
- the TRPV6 channel is a key element in Ca(2+)/1,25-dihydroxyvitamin D3-induced differentiation of human keratinocytes
- a cheese whey protein digest increased the calcium uptake by the CHO-hCaT1 cells, human intestinal Caco-2 cells, and in vivo using rats with enteral feeding
- TRPV6 may be a novel target for the development of calcium channel inhibitors to treat breast adenocarcinoma expressing TRPV6.
- The ancestral gain-of-function haplotype in TRPV6 plays a role in calcium stone formation in certain forms of absorptive hypercalciuria.
- all non-African populations carry a signature of selection on the same haplotype at the TRPV6 locus, demonstrating a widespread parallel selection event acting on standing genetic variation in humans
- this study presents the first physiological function of Nipsnap1 as an associated protein inhibiting transient receptor potential vanilloid channel 6 activity that possibly exerts its effect directly at the plasma membrane
- Results suggest that TRPV6 channels in T84 cells contribute to the calcium entry/signalling pathway that is sensitive to 17beta-estradiol.
- CypB is a new TRPV6 accessory protein with potential involvement in TRPV6 channel activation through its peptidyl-prolyl cis/trans isomerase activity
- Regulation of TRPV6 gene expression by short chain fatty acids may be a molecular mechanism involved in the promotion of calcium absorption by fructooligosaccharides in rats.
- The expression pattern and the selective Ca2+ permeation properties of TRPV6 channel indicate an important role of this channel in the Ca2+ homeostasis and probably in malignant transformation of blood cells.
- These results further support the idea that the skewed polymorphism of TRPV6 is generated by recent positive selection rather than being the result of demographic events in the Guangzhou population.