|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for SLC47A1(NM_018242.2) Search again
Product ID:
HQP014217
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
MATE1
Gene Description:
solute carrier family 47 member 1
Target Gene Accession:
NM_018242.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Summary
This gene is located within the Smith-Magenis syndrome region on chromosome 17. It encodes a protein of unknown function.
Gene References into function
- MATE1 appears to be the long searched for polyspecific organic cation exporter that directly transports toxic organic cations into urine and bile.
- an oppositely directed H(+) gradient serves as a driving force of tetraethylammonium transport via rMATE1
- The results suggest that hOCT2 and hMATE1 mediate paraquat transport in the kidney.
- hMATE1 and hMATE2-K function together as a detoxication system, by mediating the tubular secretion of intracellular ionic compounds across the brush-border membranes of the kidney.
- Sp1 functions as basal transcriptional regulator of human and rat MATE1 gene through two GC boxes. May be conserved among species. We have identified rSNP of hMATE1 gene (G-32A) (belonging to Sp1-binding site) that affects promoter activity.
- The molecular basis of substrate recognition by MATE1 was investigated via amino acid substitution in the conserved transmembrane regions.
- Guanin/adenine single nucleotide polymorphism is associated with a reduction in A1C level suggesting it role in the pharmacokinetics of metformin in diabetics.