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Validated All-in-One™ qPCR Primer for PPP2CA(NM_002715.3) Search again
Product ID:
HQP014115
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
HJS3, NEDLBA, PP2Ac, PP2CA, PP2Calpha, RP-C
Gene Description:
protein phosphatase 2 catalytic subunit alpha
Target Gene Accession:
NM_002715.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes the phosphatase 2A catalytic subunit.
Gene References into function
- A functional role for the B56 alpha-subunit of protein phosphatase 2A in ceramide-mediated regulation of Bcl2 phosphorylation status and function
- regulated by hydrogen peroxide and glutathionylation
- role in p38 mitogen-activated protein kinase-mediated regulation of the c-Jun NH(2)-terminal kinase pathway in human neutrophils
- Results describe the cell cycle expression, subcellular distribution, and metabolic stability of protein phosphatase 2Abeta in comparison with 2Aalpha.
- PP2Calpha has a role in cell cycle regulation and p53 activation
- roles of conserved active-site amino acids within PP2Calpha
- the A subunit and alpha-4 (mTap42) require charged residues in separate but overlapping surface regions to associate with the back side of PP2Ac and modulate phosphatase activity
- Studies suggest that the target of regulation by PP2A includes upstream kinases in the JNK MAPK pathway.
- We propose that stabilization of this inactive, nuclear PP2A pool is a major in vivo function of PME-1.
- down-regulation of expression of PP2A catalytic subunit in CAPE treated cells
- PP2Calpha may possess tumor-suppressing properties, and it thereby sets the stage for more elaborate analyses on its involvement in the development and progression of cancer.
- PP2A functions as a negative regulator in TGF-beta1-induced TAK1 activation
- study found that the level of the catalytic subunit of PP2A(PP2Ac) was dramatically decreased in adult Down syndrome brain, and this decrease correlated negatively with tau leveland phosphorylation at several abnormal hyperphosphorylation sites
- Aurora-B is an EB1-interacting protein; EB1 stimulates Aurora-B activity through antagonizing its dephosphorylation/inactivation by PP2A
- These data shed new light on the significance of negative feedback regulation of NF-kappaB and identifies PP2A as the key regulator of this process.
- 1) PAK plays a required role in hyperosmotic signaling through the PI3K/pTEN/Cdc42/PP2Calpha/p38 pathway, and 2) PAK and PP2Calpha modulate the effects of this pathway on focal adhesion dynamics.
- PP2A appears to function as an endogenous regulator of SK1 phosphorylation
- Active transport of the ubiquitin ligase MID1 along the microtubules is regulated by protein phosphatase 2A.
- The catalytic subunit of protein phosphatase 2A (PP2A-C) is the protein interacting with GPR54.
- The role of PP2A in the phosphorylation of P301L mutant human tau and in neurofibrillary tangle pathology in vivo was determined.