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Validated All-in-One™ qPCR Primer for ACP5(NM_001611.3) Search again
Product ID:
HQP013957
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
HPAP, TRACP5a, TRACP5b, TRAP, TRAcP, TrATPase
Gene Description:
acid phosphatase 5, tartrate resistant
Target Gene Accession:
NM_001611.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq].
Gene References into function
- Serum tartrate-resistant acid phosphatase isoforms in rheumatoid arthritis.
- One isoenzyme, but not the other (5b but not 5a) correlates with other markers of bone turnover and bone mineral density.
- serum tartrate resistant tartrate resistant acid phosphatase 5 is useful as a marker for bone resorption
- specific and sensitive marker of bone resorption and for the early detection of the spreading of breast cancer cells to bone
- Increased tartrate-resistant acid phosphatase isoform 5b is associated with multiple myeloma bone disease
- In human serum, TRACP 5b circulates in a large complex that contained alpha2M and calcium.
- The Human serum tartrate-resistant acid phosphatase exists as two enzyme isoforms (TRACP 5a and 5b), derived by differential, post-translational processing of a common gene product.
- Elevated tartrate-resistant acid phosphatase 5b in serum is associted with extensive bone metastasis in breast cancer
- Results lead to hypothesize that the capacity of osteoblast-like cells to endocytose TRACP (ACP5) is important for the removal of this enzyme during or following the bone resorptive activity of the osteoclast.
- a role of the loop residue D158 in catalysis in the cleaved enzyme.
- crystal structures at 2.2A resolution demonstrate that the repression loop exhibits significant conformational flexibility, and the observed alternate binding mode suggests a possible inhibitory role for this loop (purple Acid phosphatase)
- The results suggest that in endothelial cells of the afferent arterioles, mesangial cells, and lymphocytes the cellular activities are regulated by high constitutive phosphotyrosine phosphatase.
- the MCP-1-induced TRAP(+)/CTR(+) multinuclear cells represent an arrested stage in osteoclast differentiation, after NFATc1 induction and cellular fusion but prior to the development of bone resorption activity
- These results suggest that the protease-sensitive loop peptide, redox-active iron, and disulfide bond are important regulatory sites in TRACP.
- TRAP might be useful as a marker of progression of malignant disease and could be a potential target for cancer therapies.
- This work suggests that tumour derived TRAP contributes to the raised enzyme activity found in the serum of breast cancer patients.
- TRAP 5b may serve as a new additional marker of bone resorption in the assessment of renal osteodystrophy.
- heterozygous mutation (R714C) of the osteopetrosis gene, pleckstrin homolog domain containing family M (with run domain) member 1 (PLEKHM1), impairs vesicular acidification and increases TRACP secretion in osteoclasts
- Myeloma-osteoclast interactions stimulated the production of TRAP, cathepsin K, MMP-1, -9, and uPA
- Over-expression of monomeric tartrate resistant acid phosphatase, but not the dimeric form in adipose tissue leads to early onset spontaneous hyperplastic obesity
- This study was undertaken to examine the significance of serum TRACP isoforms 5a and 5b as disease markers of inflammation and bone destruction in rheumatoid arthritis.
- These results indicate that the differences between TRAP 5a and 5b consist not only of a difference in modification by sialic acid but differences in other sugar chain structures.
- Data show that tartrate-resistant acid phosphatase activity is modulated during osteoblastic differentiation, possibly in response to the redox state of the cell, since it seemed to depend on suitable levels of reduced glutathione.