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Validated All-in-One™ qPCR Primer for PLIN1(NM_002666.4) Search again
Product ID:
HQP013261
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
FPLD4, PERI, PLIN
Gene Description:
perilipin 1
Target Gene Accession:
NM_002666.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq].
Gene References into function
- Perilipin could be a factor behind impaired lipolysis in insulin-resistants sconditions.
- perilipin was elevated in obese subjects, perhaps as a compensatory mechanism to limit basal lipolysis.
- Transcription of the human perilipin gene is stimulated by peroxisome proliferator-activated receptor-gamma (PPAR-gamma) through a DR-1 type PPRE.
- Genetic variation at the perilipin (PLIN) locus is associated with obesity-related phenotypes in White women
- study suggested a significant contribution of perilipin(PLIN) polymorphism 1243 to the elevated total cholesterol (TC) levels indicating that PLIN gene may be involved in human lipid metabolism
- data support the hypothesis that the perilipin(PLIN) locus may be a significant genetic determinant for obesity risk in whites and that women are more sensitive to the genetic effects of perilipin than men
- The association of increased obesity risk and structure of PLIN gene is studied; results support the role of the PLIN locus as an ethnically dependent modulator of obesity risk in humans.
- We show the presence and induction of perilipin in atheroma.
- PLIN11482A carriers were resistant to weight loss, suggesting that this polymorphism may predict outcome of BW reduction strategies based on low-energy diets.
- Perilipin targets a novel pool of lipid droplets for lipolytic attack by hormone-sensitive lipase
- Cooperation with other transcription factors may be differentially involved in selective transactivation of the perilipin gene by different peroxisome proliferator-activated receptor subtypes.
- PLIN 11482G-->A/14995A-->T polymorphisms modulate the association between SFAs/carbohydrate in diet and insulin resistance in Asian women.
- Genetic variations in the perilipin gene can affect weight gain associated with rosiglitazone treatment in patients with type 2 diabetes.
- Results suggest that that PLIN constitutes a susceptibility locus for reduced BMD in Japanese men.
- The rs4578621 and rs894160 polymorphisms of the perilipin gene are not major genetic determinants of obesity and type 2 diabetes-related phenotypes in a random sample of French men and women.
- PLIN is a candidate gene for obesity risk in humans as well as a modulator of dietary response to therapies aimed to reduce body weight and decrease metabolic syndrome risk. [REVIEW]
- Perilipin content decreased and adipophilin increased with lipoprotein lipid loading regardless of intracellular neutral lipid composition
- negative finding that the common variants of PLIN do not have a major effect on susceptibility to stroke in a Chinese population
- The results of these studies demonstrated that A. phagocytophilum modulates lipid metabolism by increasing PLIN mRNA levels and facilitates infection of HL-60 cells.
- Thus, the perilipin gene expression is regulated by a transcriptional network controlling energy metabolism, substantiating the functional importance of perilipin in the maintenance of body energy balance.
- These 2 studies suggest that the presence of the minor C and A alleles at PLIN1 and PLIN4, respectively, are associated with a lower postprandial response that may result in lower atherogenic risk for these persons
- Central obesity may modify the associations between PLIN variations and diabetes risk in women.
- Perilipin, which was thought to be characteristic for lipid droplets of adipocytes and steroidogenic cells, becomes de novo expressed in hepatocytes of human, mouse, and cattle liver.
- Polymorphisms in perilipin gene (PLIN) are not associated with obesity and weight variation in people with high risk of type 2 diabetes.
- Associations between PLIN gene polymorphisms and obesity risk have been described but this study shows that interactions with dieetary carbohydrates affect waist size.
- The minor A allele at PLIN was associated with higher risk of metabolic syndrome at baseline
- Mycobacterium leprae regulates ADRP/perilipin expression to facilitate the accumulation of lipids within infected macrophages for intracellular survival.