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Validated All-in-One™ qPCR Primer for PLAU(NM_002658.4) Search again
Product ID:
HQP013204
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ATF, BDPLT5, QPD, UPA, URK, u-PA
Gene Description:
plasminogen activator, urokinase
Target Gene Accession:
NM_002658.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment.
Gene References into function
- Pleiotrophic inhibition of pericellular urokinase-type plasminogen activator system by endogenous tumor suppressive maspin.
- Cooperativity between the Ras-ERK and Rho-Rho kinase pathways in urokinase-type plasminogen activator-stimulated cell migration
- Exposure of cryptic domains in the alpha 1-chain of laminin-1 by elastase stimulates macrophages urokinase and matrix metalloproteinase-9 expression.
- TPA & urokinase progressed to maximum levels in late pregnancy, but decreased in labor, favoring a coagulation state despite elevated D-dimer levels suggesting enhanced fibrinolysis.
- significant decresase in PBM uPA activity in HIV infected and immunosuppresed patients independent from the presence of P. carinii lung infections
- Substitution of the two relevant serines with glutamic acid residues impairs the ability of urokinase to mobilize a variety of human and mouse cell lines as well as human primary T lymphocytes.
- bFGF up-regulated UPA in both normal and dystrophic myoblasts and weakly upregulated UPA receptor in normal satellite cells.
- Random peptide bacteriophage display used as a probe to identify urokinase receptor binding sites other than the binding site of UPA residues 1-48.
- In a study of the differential role of UPA and TPA as inducers of fibronectin mRNA, UPA led to an increase of FN mRNA expression in early G1, did not lead to FN assembly in the ECM, induced monomric FN
- Coculture of monocytes and vascular smooth muscle results in 5-fold increase in UPA expression in monocytes, resulting in VSMC migration.
- Osteopontin modulates prostate carcinoma invasive capacity through RGD-dependent upregulation of UPA.
- Protease activity, growth factor-like and kringle domains of uPA differentially contribute to activation of p42/p44erk1,2 and p38 MAP-kinases.
- Endogenous UPA is not only overexpressed in smooth muscle cells upon stimulation with PDGF/bFGF, but also mediates the mitogenic activity of the growth factors in a catalytic-domain-dependent manner.
- possible role of fibroblast growth factors in expression of genes of the plasminogen activator system in breast fibroblasts
- Structural analysis of the interaction between urokinase-type plasminogen activator and its receptor: a potential target for anti-invasive cancer therapy. Review.
- Diagnostic significance of measurement of the receptor for urokinase-type plasminogen activator on granulocytes and in plasma from patients with paroxysmal nocturnal hemoglobinuria.
- interaction with urokinase receptor mediates inhibitory signal for HIV-1 replication
- expression of uPA and uPAR is associated with the clinical behaviour of bladder neoplasms
- Increased expression of urokinase during atherosclerotic lesion development causes arterial constriction and lumen loss, and accelerates lesion growth.
- Characterization of the binding of human urokinase-type plasminogen activator to the asialoglycoprotein receptor of rat liver
- Urokinase plasminogen activator receptor could be associated with BBB disruption and with promotion of CNS invasion by chemotactically active cells, macromolecules, and microbes.
- upregulation of PAI-1, uPA, and tPA after long-term LDL exposure seems to be mediated by a delayed PKC activation associated with an increased PA inhibitory activity
- the circluating level and its soluble receptor (suPAR) are not up-regulated by the circulating P105 fraction of the HER-2/neu proto-oncogene: in vivo evidence from patients with advanced NSCLC
- the role of urokinase in the regulation of monocyte/macrophage functions, such as that occurring in inflammatory reactions
- There was a positive correlation between uPA and PAI-1 antigen levels and clinicopathological parameters such as grade (p < 0.001 and p = 0.01, respectively)
- uPA mrna stability is maintained through shuttling of uPA mRNABp between the nucleus and cytoplasm
- u-PA present on the platelet surface interacts with u-PAR on the endothelial cell and induces u-PA synthesis in the vascular enodthelial cell.
- promotion of mRNA stability in invasive brease cancer cells by Rac1-MKK3-p38-MAPKAPK2 pathway
- recognition of uPA by alpha(M)beta(2) allows for formation of a multicontact trimolecular complex, in which a single uPA ligand may bind to both uPAR and alpha(M)beta(2); interaction of uPA with each receptor influences cell adhesion and migration
- C/C genotype in exon 6 of uPA gene can be further considered as being related to colorectal cancer progression.
- Syk, a protein-tyrosine kinase, suppresses the cell motility and nuclear factor kappa B-mediated secretion of this enzyme by inhibiting the phosphatidylinositol 3'-kinase activity in breast cancer cells.
- recombinant kringle domain has anti-angiogenic activity
- small, hormone-receptor-positive breast cancers (with a theoretical good prognosis) may carry an elevated risk of nodal involvement if accumulation of uPA-PAI-1 complexes is shown inside their tumour cells by means of immunohistochemistry.
- Human/chicken chimeric uPA molecules & point mutants revealed the structural requirements for uPA autoactivation vs zymogen stability. The amino terminal fragment of chicken uPA engineered onto the human uPA molecule induces human uPA autoactivation.
- PDGF and similar cytokines may be important factors in airway remodeling by redistribution of smooth muscle cells during inflammation and that urokinase may be important in potentiating the response.
- Induction of PAI-1 by exposure of lung epithelial cells to uPA is a newly recognized pathway by which PAI-1 could regulate local fibrinolysis in lung inflammation or neoplasia
- High expression of Urokinase-type plasminogen activator is associated with melanoma
- uPA regulates uPAR expression in NSCLC at a posttranscriptional level by increasing uPAR-stability through a cellular factor that binds the coding region of uPAR-mRNA
- The expression of uPA was significantly correlated with depth of gastric tumor invasion, differentiation, lymphatic and vascular invasion.
- tPA and plasma kallikrein-mediated uPA activation and tPA release contribute to endogenous fibrinolytic or thrombolytic mechanisms.
- evidence that the small GTPases RhoA and Rac1, but not Cdc42, are directly associated with Tyk2 and PI3-K in an uPA/uPAR-dependent fashion and are necessary to mediate the uPA/uPAR-directed migration via the Tyk2/PI3-K signalling complex in human VSMC
- Plasma uPA levels are greater in those with bladder cancer than in healthy subjects (P <0.001); for patients with bladder cancer, a greater preoperative plasma uPA level was an independent predictor of poor outcome after radical cystectomy.
- A high cytosol value of this enyme may be predictive of early relapse in primary breast cancer.
- increased expression of urokinase-type plasminogen activator(uPA) may initially lead to degradation of amyloid beta-protein but chronic expression of uPA and plasminogen activation led to loss of cerebrovascular smooth muscle cell attachment
- The urokinase-type plasminogen activator (uPA)-plasmin cascade is involved in the activation of astrocyte pro-MMP-2 in glioma-astrocyte coculture that leads to the increased invasiveness of glioma cells in the central nervous system.
- first report that osteopontin induces nuclear factor kappaB activity and urokinase secretion by activating phosphatidylinositol 3'-kinase/Akt protein/Ikappab kinase-mediated signaling pathways
- Methylation status of uPA promoter regulates prostate cancer invasion and growth.
- The increase in antigenic levels of uPA and MMP-3 in endometrium of women with endometriosis might contribute to the invasive potential of endometrial cells
- data indicating that the structure of the kringle as well as its interaction with the growth factor domain govern urokinase binding to the urokinase receptor
- the promoter activity of uPA is enhanced in gastric tumor cells by p38 MAPK
- PLAU is a promising new candidate gene for late-onset Alzheimer's disease, with allele C (P141) being a recessive risk allele or allele T (L141) conferring protection.
- Increased expression of u-PA is a feature of bone metastasis in patients with prostate cancer.
- confirm the role of uPA in acantholysis and suggest an involvement of IL-1alpha/TNF-alpha in uPA induction
- TAFIa inhibited lysis of model thrombi and plasma clots by uPA, scuPA and tPA, which could be partially overcome by plasminogen, consistent with TAFIa exerting its effect through modifying the binding of plasminogen and tPA to fibrin.
- predictors of human immunodeficiency virus (HIV) disease progression (review)
- No significant increases in uPAR were found in pretreatment determinations of patients with advanced small-cell lung cancer or non-small-cell lung cancer.
- urokinase and urokinase receptor mRNAs are stabilized by HuR linked to its cytoplasmic accumulation induced by activated mitogen-activated protein kinase-activated protein kinase 2
- uPA and uPAR are up-regulated during acute renal allograft rejection
- nuclear factor kappaB-mediated secretion of urokinase type plasminogen activator is regulated by p56lck through tyrosine phosphorylation of IkappaBalpha following hypoxia-reoxygenation
- uPA is upregulated by TGF-beta1 and PI3 kinase in human tumor cells
- Increased uPa hydrolysis was associated with endometriotic lesion in patients diagnosed with endometriosis.
- secretion is induced by osteopontin inducing AP-1 through c-Src-dependent epidermal growth factor receptor transactivation in breast cancer cells
- uPA:PAI-1 complex independently predicts the efficacy of adjuvant chemotherapy in patients with primary breast cancer.
- suppression of activation of receptor-bound pro-urokinase leads to downregulation of matriptase and inhibition of tumor invasion
- assembly of Plg and uPA on integrin alpha(M)beta(2) regulates Plm activity and, thereby, plays a crucial role in neutrophil-mediated thrombolysis
- uPA and PAI-1 have roles in progression and recurrence of breast cancer [review]
- urokinase-type plasminogen activator (uPA) and type-1 inhibitor (PAI-I) complex has a role in breast cancer resistance to endocrine therapy independent of steroid hormone receptor status
- MMP-13, uPA, and PAI-1 antigen levels were determined in the synovium of patients with osteoarthritis
- The urokinase gene 3'-UTR "T" allele was associated with rheumatoid arthritis in Chinese patients in Taiwan.
- investigated whether the increase of uPA monocyte expression and chemokine releases induced by oxidised low density lipoproteins involved proinflammatory phospholipid products having platelet-activating factor-like activity via the PAFR pathway
- u-PA down-regulation by RNAi technology decreases the invasive capability of hepatocellular carcinoma cells
- NF-kappaB dysregulation and uPA overexpression have roles in a more aggressive tumor behavior in hepatocellular carcinoma, and IKKbeta plays a critical role in the HBx-activated NF-kappaB signaling pathway
- Endothelial uPA expression is flow sensitive and differentially regulated by laminar and turbulent shear stress in vitro. This effect may contribute to local nature of atherosclerosis.
- Data describe the cyclic variation and distribution of urokinase plasminogen activator (uPA), uPA receptor and plasminogen activator inhibitor 1 (PAI-1) mRNA in normal endometrium.
- Patients with mitral valve prolapse have a higher frequency of PLAU polymorphism among the chinese population in Taiwan.
- uPA/uPAR ligation anchors the complex to the actin cytoskeleton and is a part of the mechanism responsible for uPA-induced cell migration in fibroblasts.
- The urokinase gene 3'-UTR "T" allele is associated with oral cancer in humans, and may play a role in oral cancer formation.
- participates in endothelial cell migration under regulation of p38 MAPK pathway
- Increased expression of urokinase-type plasminogen activator is associated with transitional cell carcinoma of the human bladder
- Urokinase (uPA) is a potent chemoattractant for basophils that seems to act through exposure of the chemotactic uPA receptor epitope uPAR84-95, which is an endogenous ligand for formyl peptide receptor (FPR)-like 2 (FPRL2) and FPRL1.
- uPA mRNA and uPAR mRNA expression were found in 9 and 8 of prostsatic adenocarcinomas, respectively, and in 7 and 6 of benign prostatic hyperplasias.
- Polymorphisms are not associated with late onset Alzheimer's disease.
- sesamol increased the production of uPA and tPA significantly and also up-regulated the mRNA expressions of these proteins.
- VEGF and PAI-1 interact and may have roles in progression of node-negative breast cancer
- the G(i)-Ras-Raf-NF-kappaB signaling cascade is responsible for LPA-induced uPA up-regulation in ovarian cancer cells
- expression and assembly of uPAR co-receptors in a specific cell type determines the response to uPA
- basic capacity of separated epithelial and stromal cells from all three types of tissue to release uPA, PAI-1, and suPAR without any paracrine influence, as in vivo
- IGF-I and HGF cooperate to induce migration and invasion of colorectal carcinoma cells, and c-Met and uPA/uPAR are required for IGF-I-mediated migration and invasion.
- sphingosylphosphorylcholine may affect angiogenesis in the wound-healing process via regulation of uPA production
- Our results suggest that PBK1 is involved in the regulation of uPA expression, which might provide a new clue to further understanding the regulation mechanism of uPA expression.
- expression and regulation of uPA mRNA. This pathway may regulate uPA-mediated functions of the lung epithelium in the context of inflammation or neoplasia
- The level of urinokinase-type plasminogen activator (u-PA) does not correlate with cerebrospinal fluid to serum albumin ratio, suggesting an unimportant role for uPA in HIV-induced blood-brain-barrier disruption.
- Epidermal growth factor modulates prostate cancer cell invasiveness by regulating urokinase-type plasminogen activator activity
- p53 is induced by urokinase in lung epithelial cells
- the urokinase-type plasminogen activator (uPA) induces, via its specific receptor (uPAR, CD87), upregulated expression of the complement anaphylatoxin C5a receptor
- uPA processes recombinant latent platelet-derived growth factor D into the active form.
- results demonstrate that endogenous HMW uPA inhibits S. aureus growth both directly, by cytolysis, and indirectly, by abrogation of the neutralizing effect of staphylokinase on the bactericidal activities of human neutrophil peptides
- decrease in uPAR mRNA and protein concentrations caused by DNA enzymes significantly suppressed Saos-2 cell invasion as assessed by an in vitro Matrigel assay
- Urokinase-type plasminogen activator is overexpressed in renal cell carcinoma and could function as tumour marker.
- the uPA gene contains a novel 13-bp palindrome that contains the DNA consensus-binding hexamer for RUNX1
- Elevated plasma levels in, and therefore a marker for cancers.
- a complex signaling network operating downstream of uPA-uPAR actively advances tumor cell invasion, proliferation, and survival of prostate cancer cells
- Induced-sputum innate immune factor levels discriminate inflammatory changes in cystic fibrosis, COPD, and asthma.
- Both wild-type (wt) and human cleavage resistant-uPAR are able to mediate uPA-induced migration, are constitutively associated with the EGFR, and associate with alpha3beta1 integrin upon uPA binding
- crystal structure at 1.9 angstroms of the urokinase receptor complexed with the urokinase amino-terminal fragment and an antibody against the receptor
- the urokinase/PAI-2 complex is a new high affinity ligand for the endocytosis receptor low density lipoprotein receptor-related protein
- data provide evidence for a novel pathway of fibronectin matrix assembly through the uPAR-dependent sequential activation of Src kinase, EGFR, and beta1 integrin
- IL-1alpha can induce selective upregulation of alpha6beta1-integrin and uPA/uPAR in pancreatic cancer cells and these changes may modulate the aggressive functions of pancreatic cancer.
- The T allele of 3' UTR of the urokinase gene may not be associated with a higher risk of stone formation.
- Results clearly suggest the involvement of the uPA-uPAR system in cell survival and proliferation in addition to their role in tumor progression.
- urokinase plasminogen receptor domain III controls a functional association with alpha5beta1 integrin and tumor growth
- endothelial progenitor cells are characterized by high intrinsic uPA/uPAR-dependent proteolytic potential that could contribute to their invasive and angiogenic behaviour
- In adult alphaMUPA mice (uPA transgenic) the incisor teeth display discoloration and tip fragility, and reduction of the outer enamel as determined by scanning electron microscopy.
- incubation of retinal pigment epithelial cells with uPA elicits a time-dependent enhancement of MAPK and phosphatidylinositol 3-kinase activities as well as the transcriptional activation of Bcl-xL anti-apoptotic factor
- Intratumoral injections of the plasmid vector expressing siRNA for uPA resulted in regression of pre-established, subcutaneous meningioma tumors.
- Effects induced by exogeneous addition of domain-specific uPA antibodies and uPA-interacting molecules on proliferation of uPA-suppressed MDA-MB-231 breast cancer cells.
- Constitutive uPA primes, and exogenous uPA can activate, airway eosinophil adhesion following segmental allergen challenge and that increased uPA expression may be a mechanism of increased eosinophil infiltration and function in asthma.
- Native electrophoresis and SDS-PAGE showed multiple protein bands corresponding to different forms of the urokinase, which were confirmed by Western blotting and zymography.
- down-regulation of matriptase expression in THP-1 cells by siRNA reduced the activation of cell-associated pro-uPA and the subsequent rapid initiation of plasminogen activation
- preliminary crystallographic analysis of amino-terminal fragment
- Lipoprotein receptor-related protein -mediated clearance of uPA is one of the mechanisms involved during the control of human thyroid carcinoma cell invasion.
- Evidence of a genetic and functional involvement of a common PLAU variant into the pathogenesis of Alzheimer Disease.
- review of the expression of the plasminogen activator system in endometriosis
- urokinase-type plasminogen activator (uPA) and its soluble receptor may not have roles in progression of atopic eczema/dermatitis syndrome, as plasma levels of uPA and suPAR are similar in patients at different stages of AEDS and healthy subjects
- the ability of hepsin to efficiently activate pro-uPA suggests that it may initiate plasmin-mediated proteolytic pathways at the tumor/stroma interface that lead to basement membrane disruption and tumor progression
- study shows uPA & uPAR are overexpressed in primary prostate cancer cells & surrounding stromal cells (Gleason score >/=7) & more than 90% lymph node metastases but not in normal prostate, benign prostate hyperplasia or prostatic intraepithelial neoplasi
- distribution of four tagSNPs (rs2227562 in intron 5, rs2227564 in exon 6, rs2227571 in intron 9, and rs4065 in 3'UTR) in the PLAU gene in a large case-control study of Alzheimer's disease
- autotaxin induces uPA expression via the Gi-PI3K-Akt-NF-kappaB signaling pathway that might be critical for autotaxin-induced tumor cell invasion and metastasis
- uPA gene regulation by c-phycocyanin (c-pc) is transcriptionally controlled through cAMP mediated PKA pathway.
- plasmin-mediated apoptosis of VSMC occurs via plasminogen activation by either t-PA or u-PA and is impaired by PAI-1.
- The blood concentrations of soluble urokinase-type plasminogen activator (uPAR) and mannan-binding lectin (MBL) are related to the risk of postoperative infectious complications.
- Our results showed that the subcutaneous injection of small interfering RNAs (siRNA) u-PA SKHep1C3 stable transfected cells (pS siRNA u-PA) led to a growth delay in xenograft development.
- role for PLAU_1 as an independent genetic risk factor for Alzheimer disease in the Italian population for those subjects who do not have the APOE-epsilon4 allele
- This study suggests that ILK serves as a key mediator in TGFbeta1 regulation of uPA/PAI-1 system critical for the invasiveness of human ovarian cancer cells. And ILK is a potential target for cancer therapy.
- Urokinase gene 3'-UTR polymorphism associated with prostate cancer.
- LRP is expressed in colorectal cancer and forms complexes with u-PA and its inhibitor
- alpha2-antiplasmin induction inhibits E-cadherin processing mediated by the plasminogen activator/plasmin system, leading to suppression of progression of oral squamous cell carcinoma via upregulation of cell-cell adhesion
- recombinant kringle domain inhibits the malignant glioma growth by suppression of angiogenesis
- uPA may play a critical role in ligation-induced vessel remodeling
- PAI-3, but not PAI-1 or uPA, may have a role in breast cancer survival by suppression of tumor invasion through protease inhibition in stroma
- Elevation of plasma uPA and uPAR levels in CaP patients are partly caused by local release from the prostate. Plasma levels of uPA and uPAR are associated with biologically aggressive CaP, disease progression after radical prostatectomy, and metastasis
- The production of matrix metalloproteinases (MMPs), tissue inhibitors of MMPs, and plasminogen activators by gingival fibroblasts stimulated with lipopolysaccharides produced by periodontopathogens, are reported.
- Upregulation of urokinase type plasminogen activator (UroPA) expression during early development of knee osteoarthritis (OA) occurs via MAPK ERK, JNK, and p38 signaling pathways and the PI3K signaling pathway in cultured cells debrided from OA knees.
- The association between tissue factor and uPA/suPAR system is significantly related to the presence of cardiovascular disease in hemodialysis patients
- PLAUR and the somatomedin B region of VTN direct the localization of UPA to focal adhesions in microvessel endothelial cells.
- The PLAU gene codes for this enzyme, and is located on 10q24. This region has demonstrated evidence for linkage in a genome scan for asthma in a sample from northeastern Quebec.
- In thyroid cells, plasminogen activators are regulated via MAPK and protein kinase c-beta pathways.
- Altered expression of PLAU was observed in high-risk soft tssue sarcomas.
- pemphigus vulagris-IgG-dependent uPA activation is not related to anti-Dsg3 antibody activity
- stromal fibroblasts play a role in promoting pancreatic cancer metastasis via activation of the uPA-plasminogen-MMP-2 cascade
- suggest a novel function for tyrosine kinase 2 as an important modulator of the urokinase-directed vascular smooth muscle cell functional behaviour at the place of injury
- Simultaneous down-regulation of uPAR and uPA induced caspase-8 mediated apoptosis.
- The urokinase plasminogen activator (BamHI) and plasminogen activator inhibitor type 1 (HindIII) genotypes may serve as useful markers for heritability of bone loss associated with periodontal disease.
- STI571 influence through inducing urokinase-type plasminogen activator and interleukin-8, in which the induction of early growth response-1 expression and extracellular signal-regulated kinase 1/2 phosphorylation might be involved.
- the p38(MAPK) signaling pathway is required for optimal LPA-dependent uPA secretion from ovarian cancer cells
- silencing of uPA significantly inhibits prostate tumor growth and the incidence of lung metastasis
- we demonstrated that tyrosine phosphorylation of EGFR and the autocrine expression of uPA and HB-EGF depend on the activity of c-jun amino-terminal kinase (JNK) in human prostatic DU-145 cells.
- simultaneous downregulation of uPAR and matrix metalloprotease-9 using RNAi technology may provide an effective tool for breast cancer therapy
- Urokinase plasminogen activator (uPA) stimulates cholesterol biosynthesis in macrophages through activation of SREBP-1 in a PI3-kinase and MEK-dependent manner
- uPA plays a major role in inducing MMP-1 production in activated human monocytes, which is mediated by a plasmin-dependent mechanism involving signaling through the annexin A2 heterotetramer.
- review of uPA - uPAR interactions with membrane proteins to modify signal transduction pathways and their role in oral squamous cell carcinoma
- data of study population indicate a higher ETS2 RNA concentration compared with uPA in the case of bladder cancer, resulting in an increased ETS2:uPA RNA ratio in urine
- HDAC1 knockdown by small interference RNA stimulated uPA expression and cancer cell invasion.
- Elevated expression of MMP-1 and urokinase plasminogen activator can be used to identify patients with a worse prognosis in skull base chordoma.
- In xenograft glioblastoma mouse model and in vitro invasion assay, LRP-deficient cells, which secreted high levels of uPA, invaded the surrounding normal brain tissue, whereas the LRP-overexpressing and uPA-deficient cells did not invade normal brain.
- The urokinse haplotype CGCCCC is a genetic risk fctor for higher mortality and more severe clinical outcome with infection-associated acute lung injury.
- The carotid atherosclerosis is independently related to uPA/its soluble receptor system in dialysis patients
- Myeloma-osteoclast interactions stimulated the production of TRAP, cathepsin K, MMP-1, -9, and uPA
- The multimarker phenotype of the absence of CD8+ TILs, loss of p27, and positive uPA expression was predictive of an adverse prognosis in patients with lymph node-negative, MMR-proficient colorectal cancer.
- protein kinase C synergistically enhances the secretion of uPA in TNF-alpha-stimulated human dental pulp cells
- In human renal tissues, protein C inhibitor and urinary plasminogen activator colocalized in the cytoplasm of renal proximal tubular epithelial cells
- Data show that fibroblastic inflammatory reaction around the biopsy channel affects stromal uPA and PAI-1 expression, which possibly leads to falsely increased enzyme levels in ELISA.
- IL-1beta stimulates uPA production via activation of NFkappaB and tyrosine phosphorylation, and also secretion of the enzyme, and that the uPA/plasmin system appears to be involved in inflammation in human dental pulp.
- Each additional copy of the minor allele PLAU-3995 (Pro41Leu,(rs2227564) was associated with higher log(D-dimer) levels.
- Together, the data indicate that SAA enhances plasminogen activation and suggest its possible role in plasmin(ogen)-mediated colon cancer progression.
- Inactivation of FOXO3a after GR activation is an important mechanism contributing to GC-mediated repression of uPA gene expression in breast epithelial and cancer cells.
- Urokinase 3"-UTR T/C gene polymorphism seems to appear more commonly in children with recurrenct calcium oxalate stone disease than in healthy children and in those with stones for the first time.
- TSP-1 promotes human follicular thyroid carcinoma cell invasion mainly through up-regulation of the urokinase-dependent activity
- SAK reduces tPA/uPA-mediated Plg activation by means of SAK.Plg complex formation, consequently downregulating tPA/uPA-induced fibrinolysis.
- upon cell binding and internalization, single-chain uPA (scuPA) translocates to the nucleus within minutes. Nuclear translocation does not involve proteolytic activation or degradation of scuPA.
- uPA-mediated direct activation of MMP-9 may promote glioblastoma cell invasion.
- A composite role of vitronectin and urokinase in the modulation of cell morphology upon expression of the urokinase receptor.
- UPA expression was significantly up-regulated in pancreatic ductal adenocarcinoma.
- reports the crystal structures of uPAR in complex with both urokinase (uPA) and vitronectin and reveal that uPA occupies the central cavity of the receptor, whereas vitronectin binds at the outer side of the receptor
- observations confirm a new role for p53 as a uPA mRNA binding protein that down-regulates uPA mRNA stability and decreases cellular uPA expression
- Urokinase plasminogen activator upregulates paraoxonase 2 expression in macrophages via an NADPH oxidase-dependent mechanism.
- The expressions of both uPA mRNAs in the gastric cancer cells increased obviously (12-fold and 3-fold, respectively) with H. pylori stimulation.
- The pathogenesis of idiopathic pulmonary fibrosis is complex & the urokinase-type plasminogen activator (uPA)/plasminogen system participates in the repair process. Review.
- The results of this study support previous observations linking PAI-1 with airway remodeling and uPA with cellular inflammation. Moreover, the observed effect of uPA seems to be independent of its fibrynolytic activity.
- uPA expression in metaplastic, dysplastic, and esophageal cancer tissue is associated with neoplastic progression of esophageal lesions
- Report increased expression of uPA by Helicobacter pylori in gastric epithelial cells.
- Determination of serum CATB and plasma UPA levels may be useful to identify patients at a higher risk for progression to cancer.
- uPA-binding loops of ECRG2 are in correspondence with the reactive site loops for binding of serine proteinase in turkey ovomucoid third domain (OMTKY3).
- uPA/uPAR downregulation inhibits radiation-induced migration, invasion and angiogenesis in IOMM-Lee meningioma cells and decreases tumor growth in vivo.
- suPAR, prevents the interaction of urokinase with membrane-anchored uPAR on residual normal cells.
- mutation in an uncharacterized cis element near PLAU is the cause of Quebec platelet disorder
- uPA not only generates active PDGF-DD, but also regulates its spatial distribution, provides novel insights into the biological function of PDGF-DD.
- overexpression of uPA in Quebec platelet disorder emerges with megakaryocyte differentiation, without altering the expression of flanking genes
- These results demonstrated that in A549 cells, activation of p50/p65 heterodimer through sequential activation of PKC alpha-JNK-NIK-IKK beta-NF-kappaB was required for IL-1 beta-induced uPA expression associated with migration of tumor cells.
- uPA exon 6 C/T polymorphism is associated with invasive phenotype of gastric cancer, but not with susceptibility or survival of gastric cancer. uPA intron 7 is a silent polymorphism.
- analysis of urokinase, urokinase receptor and plasminogen activator inhibitor-1 in colon cancer liver metastases
- Report recombinant production of a hybrid plasminogen activator composed of surfactant protein B and low-molecular-weight urokinase.
- Urokinase & its receptor UPAR exert a regulatory effect on physiologic cell migration as well as tumor growth and metastasis. Review.