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Validated All-in-One™ qPCR Primer for PLA2G4A(NM_024420.2) Search again
Product ID:
HQP013195
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
GURDP, PLA2G4, cPLA2, cPLA2-alpha
Gene Description:
phospholipase A2 group IVA
Target Gene Accession:
NM_024420.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Summary
This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. [provided by RefSeq].
Gene References into function
- Expressed in human colorectal adenocarcinomas
- PLA2G5 binds to PLA2G4 to induce leukotriene synthesis in neutrophils
- Nuclear localisation was dependent on proliferation, with subconfluent cells containing higher levels of nuclear cPLA(2)-alpha than contact-inhibited confluent or serum-starved cells.
- Polymorphisms in phospholipase A2 gene is associated with type 2 diabetes mellitus
- group IVA cytosolic phospholipase A(2) is activated by phosphorylation
- The proposed model for membrane docking of the C2 domain of cytosolic phospholipase A2 is fully compatible with the biological function of the intact enzyme.
- novel mechanisms involving accessory proteins at the target membrane play a role in the regulation of cPLA2-alpha
- show that Phospholipase A(2) type IVA is present in red cells as a 90-kDa protein
- cPLA2 has a role in B-Myb-dependent regulation of c-Myc expression
- cPLA(2)alpha translocated to forming phagosomes, surrounding the zymosan particle and completely overlapping with early endosome and plasma membrane markers but only partially overlapping with resident endoplasmic reticulum proteins
- Stable expression of human groups IIA and X secreted phospholipases A(2) (hGIIA and hGX) in CHO-K1 and HEK293 cells leads to serum- and interleukin-1beta-promoted arachidonate release.
- cPLA(2)beta is not critical for the intracellular signaling cascades initiated by FcgammaRI
- SNP4 located in the 5'-flanking region of the PLA2G4A gene was associated with schizophrenia
- The IValpha cytosolic phospholipase A2 at protein levels in articular cartilage from patients with rheumatoid arthritis, osteoarthritis and patients with non-arthritic joints.
- cytosolic phospholipase A2alpha (cPLA2alpha)-mediated prostaglandin E2 (PGE)2/EP1 and peroxisome proliferator-activated receptor-gamma (PPAR-gamma)/Smad signaling pathways in human liver cancer cells are activated by TGFbeta
- cPLA2 has an influence on IL-8 and COX 2 gene and protein expression at least in part through PPAR-gamma
- Results suggest that a protein kinase C delta-reactive oxygen species-NF-kappaB cascade plays a pivotal role in cytosolic phospholipase A(2)alpha induction by phorbol 12-myristate 13-acetate.
- cPLA(2)-alpha has a role in the regulation of neutrophil-mediated bacterial killing and the innate immune response to bacterial infection
- Group X secretory phospholipase A2 induces potent arachidonic acid release without activation of cPLA2a.
- Increased cPLA(2)alpha activity is associated with colon cancer
- phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] promotes translocation of cPLA2alpha to perinuclear membranes of intact cells in a manner that is independent of rises in the intracellular Ca2+ concentration
- These results suggest that early cell death events promoted by an overload of calcium can be prevented by the activity of cytosolic Group IVA phospholipase A(2)alpha.
- HCy led to the phosphorylation of cytosolic phospholipase A2; HCy promoted cPLA2 activation; cPLA2 phosphorylation and activation required p38 MAPK
- This study reviews the evidence and discusses the potential roles of phospholipase A2 Group 4A for schizophrenia with particular emphasis on published association studies.
- cytosolic and group IIA secreted phospholipases A(2) work together to liberate arachidonate from phospholipids in response to cytokines
- This review summarizes the phenotypes resulting from genetic ablation of cPLA2alpha, and the properties of newly discovered enzymes in the cPLA2 family.
- Our data suggest that PKCalpha, but not PKCbeta, is the predominant cPKC isoenzyme required for cPLA(2) protein phosphorylation and maximal induction of cPLA(2) enzymatic activity upon activation of human monocytes.
- PPARdelta induces COX-2 expression and the COX-2-derived PGE(2) further activates PPARdelta via cPLA(2)alpha. which forms a growth-promoting signaling that may play a role in hepatocarcinogenesis.
- The data presented link the stimulation of ERK-cPLA(2)-15-LO pathway by oxidized LDL to the prooxidant mechanism of the lipoprotein complex.
- Residues specific for binding arachidonic and palmitic acids, preferred substrates for cPLA2 and PLB1, respectively, are identified. These results explan the differences in substrate specificity between lipases sharing the cPLA2 catalytic domain fold.
- the confluence-dependent interaction of cPLA(2)alpha and annexin A1 at the Golgi acts as a novel molecular switch controlling cPLA(2)alpha activity and endothelial cell prostaglandin generation.
- This study showed that cortisol increased PLA2G4A mRNA level via GR-dependent ongoing transcription in human amnion fibroblasts by activating the binding of GR to PLA2G4A promoter directly.
- Cell surface ANXA1 synthesis is capable of blocking beta 2-integrin adhesion in PMNs. Fluticasone propionate does not cause ANXA1 synthesis or nuclear transport of cytosolic gIVaPLA2 & thus does not block beta2-integrin adhesion.
- cytosolic phospholipase A2 and leukotriene B4 may have roles in acute myeloid leukemia
- COX-2 has the ability to enhance iNOS-induced cPLA(2)alpha S-nitrosylation and maximal PG synthesis is achieved by the synergistic interaction among iNOS, cPLA(2)alpha, and COX-2
- The mutation of Ser515 to Ala (S515A) did not change cPLA(2)alpha activity, although S228A and S505A completely and partially decreased the activity, respectively.
- Role of cPLA2-alpha in the early action of TCDD through a nongenomic pathway in MCF10A cells is reported.
- human cPLA(2alpha) deficiency is associated with impaired eicosanoid biosynthesis, small intestinal ulceration, and platelet dysfunctio
- Cytosolic phospholipase A2alpha activation induced by S1P is mediated by the S1P3 receptor in lung epithelial cells.(
- BanI polymorphism of PLA2G4A showed a significant impact on mean age of the onset of schizophrenia and schizoaffective disorder in males, indicating lower mean age at admission in homozygous A2A2 males.
- Data show that activation of cytosolic phospholipase A(2) and production of lysophosphatidylcholine are initial events required for radiation-induced activation of Akt and ERK1/2 in vascular endothelial cells.
- a role of cPLA(2)alpha in the formation of nascent lipid droplets from the endoplasmic reticulum
- the inhibition of cPLA(2)alpha activity affected many of the allergen-dependent, asthma-associated gene expression changes.
- cPLA2 and IIbbeta3 interact to reinforce each other's functions during IIbbeta3 signaling.