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Validated All-in-One™ qPCR Primer for SERPINF1(NM_002615.6) Search again
Product ID:
HQP013026
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
EPC-1, OI12, OI6, PEDF, PIG35
Gene Description:
serpin family F member 1
Target Gene Accession:
NM_002615.6(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The PEDF gene is a member of the serpin gene family.
Gene References into function
- role of PEDF in protecting retinal pericytes from oxidant injury
- Results describe the first collagen-binding site described for a serpin, pigment epithelium-derived factor (PEDF), which is distinct from its heparin-binding region, neurotrophic active site, and its serpin exposed loop.
- EPC-1 may play a role in the entry of early passage fibroblasts into a G(0) state or the maintenance of such a state once reached
- reactive center loop, specifically Gly376 and Leu377, is involved in the interaction of PEDF with components of the quality control system in the endoplasmic reticulum, thus ensuring its efficient secretion
- VEGF secreted by retinal pigment epithelial cells upregulates pigment epithelium-derived factor expression via VEGFR-1 in an autocrine manner.
- pigment epithelium-derived factor content in the aqueous humor of diabetic patients strongly predicts who among them will develop progression of retinopathy
- PEDF may block the angiogenic effects of leptin through its anti-oxidative properties
- Present in plasma at approx. 100 nM (5 microg/ml) or twice the level required to inhibit aberrant blood-vessel growth in the eye
- Pterygia exhibit significantly lower PEDF but higher VEGF levels than those in normal corneas and conjunctivae. The decreased PEDF level in pterygia may play a role in the formation and progression of pterygia.
- human oral squamous cell carcinoma cells produce VEGF, which in turn regulates PEDF production, and this balance may be contributing to neovascularization in tumors.
- Gene transfer of pigment epithelium-derived factor suppresses tumor vascularization and growth, while prolonging survival in syngeneic murine models of thoracic malignancies.
- level of the natural ocular anti-angiogenic agent pigment epithelium-derived factor (PEDF) is inversely associated with proliferative retinopathy
- Malignant peripheral nerve sheath tumors (MPNST) Schwann cells lose the ability to downregulate PEDF upon axonal contact.
- effectively abates vascular endothelial growth factor-induced vascular permeability
- potential role of the age-associated decline in EPC-1 expression in tissue remodeling and in the development of skin diseases with excessive angiogenesis
- PEDF expression suggests a more favorable prognosis than in patients with ductal pancreatic adenocarcinomas.
- Role in angiogenesis of hepatocellular carcinoma(HCC). Reduction of serum PEDF concentration associated with development of chronic liver diseases may contribute to progression of HCC. Gene therapy using PEDF may be efficient treatment for HCC.
- novel role of extracellular phosphorylation is shown here to completely change the nature of PEDF from a neutrophic to an antiangiogenic factor.
- PEDF-6 affects different second messenger biosynthesis systems in HL-60 cells. and inhibits phosphatidylinositol-specific phospholipase C stimulated by aluminum tetrafluoride anions
- PEDF downregulates Fyn through Fes, resulting in inhibition of FGF-2-induced capillary morphogenesis of endothelial cells
- PEDF inhibits Ang-II-induced EC activation by suppressing NADPH-oxidase-mediated ROS generation and that PEDF may play a protective role in the development
- PEDF gene contains a response element specific for p63 and p73 in its promoter region
- PEDF may play a significant role in determining the balance of angiogenesis/ antiangiogenesis during atherogenesis
- In tumor xenografts, the overexpression of wild-type PEDF significantly suppressed tumor growth, whereas a mutant of the collagen I-binding site of PEDF (Col-mut PEDF) did not inhibit tumor growth
- In painless neuropathies, there is decreased level of PEDF in cerebrospinl fluid, compared with patients with painful neuropathies.
- Phosphorylation induces variable effects of PEDF, and therefore contributes to the complexity of PEDF action, shich might be used to generate effective antiangiogenic or neurotrophic drugs.
- REVIEW: this paper describes the unbalanced expressions of VEGF and PEDF as a cause of CNV
- Results show that low levels of PEDF in lung tumor tissues was associated with a significantly shorter survival.
- Central mechanism for PEDF inhibition of the AGE signaling to vascular permeability is by suppression of NADPH oxidase-mediated reactive oxygen species generation and subsequent VEGF expression.
- These observations collectively support the hypothesis that a lack of PEDF expression is a potent factor for the enhancement of tumor growth and angiogenesis in breast cancer.
- study is the first to demonstrate a role of pigment epithelium-derived factor (PEDF) in ovarian surface epithelium biology and ovarian cancer and suggests that the loss of PEDF may be of relevance in carcinogenesis
- PEDF may play a protective role against early diabetic retinopathy by attenuating the deleterious effect of AGE
- The analysis of the promoter region of the EPC-1/PEDF gene in this paper suggests the age- and cell cycle-dependent expression of specific transcriptional factor(s).
- Increase in serum PEDF during ulcerative colitis, especially in severe forms of disease suggests its involvement in ulcerative colitis pathogenesis.
- heparin induces a conformational change in the vicinity of Lys(178)
- High levels of PEDF in the plasma may be related to the progression of diabetic retinopathy.
- PEDF-overexpressing tumors exhibited reduced intratumoral angiogenesis, and PEDF may be a new and promising approach for the treatment of osteosarcoma.
- These results demonstrate that PEDF could inhibit neointimal formation via suppression of NADPH oxidase-mediated reactive oxygen species generation.
- hepatic PEDF levels may be elevated to counteract the effects of oxidative stress
- The correlation between PEDF and VEGF striatal levels in PD patients suggests that concerted neurotrophic functions of these factors or structural changes in blood vessel walls play an important role in the pathophysiology of PD.
- PEDF induces human umbilical vein endothelial cells apoptosis through the sequential induction of PPARgamma and p53 overexpression.
- These results demonstrate that the PEDF gene, in cooperation with the VEGF gene, may contribute to the development of diabetic retinopathy.
- PEDF induces macrophage apoptosis and necrosis through the signaling of PPARgamma.
- Results demonstrate that the aqueous humour level of asymmetric dimethylarginine is correlated with pigment epithelium-derived factor in humans and suggest that both ADMA and PEDF may be elevated in response to inflammation in uveitis.
- GPR39 protects from cell death by increasing secretion of pigment epithelium-derived growth factor
- There is a novel role for PEDF in hepatic triglyceride homeostasis through binding to ATGL. Both localize to adiposomes in hepatocytes.
- Our data suggest that the PEDF Met72Thr T allele may be a risk factor for wet AMD in the Taiwan Chinese population. PEDF may play a role in the pathogenesis of wet AMD.
- These results give the first direct demonstration that PEDF might represent a target for PARP inhibition treatment and the effects of PEDF on endothelial cells growth are context dependent.
- PEDF levels are significantly higher in type 1 diabetic patients with retinopathy compared to the patients without it.
- The levels of plasma PEDF increases with advances in both diabetic retinopathy and nephropathy.
- In moderate hypoxia, PEDF is downregulated such that the VEGF-to-PEDF ratio increases (and angiogenesis is facilitated).
- Data show that serum PEDF levels (mean (S.D.)) were increased in 96 Type 2 diabetic vs. 54 non-diabetic subjects.
- Plasma SERPINF1 level is strongly associated with body adiposity, especially with the visceral fat depot in the non-diabetic general population.
- PEDF could improve the Advanced glycation end products-elicited insulin resistance in Hep3B cells by inhibiting JNK- and IkappaB kinase-dependent serine phosphorylation of IRS-1 via suppression of Rac-1 activation.
- The HA-binding activity of PEDF may contribute to deposition in the extracellular matrix and to its reported antitumor/antimetastatic effects.
- An apoptosis-inducing factor (AIF)-related pathway is an essential target of PEDF-mediated neuroprotection.
- data suggest that none of the investigated PEDF polymorphisms is likely a major risk factor for exudative age-related macular degeneration in a white European population