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Validated All-in-One™ qPCR Primer for PDGFA(NM_002607.5) Search again
Product ID:
HQP012847
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
PDGF-A, PDGF1
Gene Description:
platelet derived growth factor subunit A
Target Gene Accession:
NM_002607.5(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene. [provided by RefSeq].
Gene References into function
- involvement of Furin gene regulation in the maturation of PDGF-AB in differentiating megakaryoblastic cells
- PDGF and similar cytokines may be important factors in airway remodeling by redistribution of smooth muscle cells during inflammation and that urokinase may be important in potentiating the response.
- The preferential formation of disulfide-bonded heterodimers from an equimolar mixture of unfolded A- and B-chains is thus a kinetically controlled process
- PDGF has a role in inducing beta-gamma-secretase-mediated cleavage of Alzheimer's amyloid precursor protein through a Src-Rac-dependent pathway
- PDGF up-regulates the expression of transcription factors NF-E2, GATA-1 and c-Fos in megakaryocytic cell lines
- a time-dependent release of TGFB and PDGF-AB occurred at neutral and alkaline pH, but not pH5, 15 min and 12h after platelet activation, suggesting relevance to wound healing
- PDGF1 potently activates AP-1 ands causes cellular neoplastic transformation.
- PDGF-A chain gene expression, under the control of KLF5, is cooperatively activated by the NF-kappaB p50 subunit and a pathophysiological stimulus.
- Glucose causes a late increase in PDGF-dependent TGF-beta 1 translation by enhancing cellular sensitivity to PDGF.
- cultured follicular keratinocytes synthesize both PDGF-A and PDGF-B, whereas, dermal papilla cells only express PDGF-A.
- PDGF-A is a novel mitogenic target of 1,25-(OH)2D3 whose expression is induced via binding of hormone-activated VDR to a response element located far upstream of the transcription start site
- PDGFA autocrine inhibition is associated with malignant gliomas
- The PDGF expression, as a marker of angiogenesis, was evaluated in myomas obtained after surgery.
- These results identify a novel mechanism of transcriptional enhancement involving ligand-independent activity of the VDR/RXR heterodimer and MAPK signaling pathways
- thrombospondin-1 is not necessary for proliferation but is permissive for vascular smooth muscle cell responses to platelet-derived growth factor
- JNK is a critical component downstream of PI 3-kinase that may be involved in PDGF-stimulated chemotaxis presumably by modulating the integrity of focal adhesions by phosphorylating its components
- 36% of the examined primary central nervous system lymphoma specimens showed expression of PDGF-A. Tumours expressing survivin occasionally co-expressed PDGF-A.
- expression level might be decreased during the secretory phase in the eutopic endometrium of women with advanced stage endometriosis
- FGF and PDGF have roles in cell proliferation and migration in osteoblastic cells
- Tretinoin inducibility of PDGFA is mediated by 5'-distal DNA motifs that overlap with basal enhancer and vitamin D response elements.
- Increased expression of PDGF/Ralphabeta may play an important role in the mechanism of growth of...paediatric fibromatous lesions
- thrombin may play an important role in the proliferation of A172 cells by inducing PDGF-AB secretion and that thrombin's action is mediated by its proteolytic activity
- Ligand-mediated stabilization of G-quadruplex structures within the PDGF-A nuclease hypersensitive element can silence PDGF-A expression.
- PDGFRs and the PDGF-A chain are frequently expressed in ovarian clear-cell adenocarcinomas.
- tumor cell-secreted platelet-derived growth factor (PDGF) and phosphoinositide 3-kinase (PI3K) activation have roles in resistance of fibroblasts against oxidative damage
- regularly expressed in variable levels in ameloblastomas
- The proteins for PDGF-A were detected in oocytes, and in granulosa cells (GC) of 50% of the follicles from women/girls.
- identified activin-mediated transforming growth factor (TGF)-beta signaling, platelet-derived growth factor (PDGF) signaling and fibroblast growth factor (FGF) signaling as the key pathways involved in MSC differentiation
- Measuring PDGFA, bFGF, and HIF1a expression may contribute to a better understanding of the prognosis of patients with pancreatic cancer.
- Results describe significant differences between controls and patients with renal cell carcinoma both pre-operatively and post-operatively in angiogenin, PDGF and MCP-1 serum levels.
- OPG production by osteoblastic cells was stimulated by platelet-derived growth factor (PDGF) in two human osteosarcoma cell lines (MG63, Saos-2), a mouse pre-osteoblastic cell line (MC3T3-E1) and human bone marrow stromal cells (hMSC).
- We showed that Prx I, PDGF-A, and PDGFR-alpha follow the same expression pattern during carcinoma ex pleomorphic adenoma progression.