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Validated All-in-One™ qPCR Primer for ANGPTL4(NM_139314.2) Search again
Product ID:
HQP012433
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ARP4, FIAF, HARP, HFARP, NL2, PGAR, TGQTL, UNQ171, pp1158
Gene Description:
angiopoietin like 4
Target Gene Accession:
NM_139314.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain.
Gene References into function
- Peroxisome proliferator-activated receptor (PPARalpha and PPARgamma) agonists decrease lipoprotein lipase secretion and glycated LDL uptake by human macrophages.
- The results suggest that homocysteine may enhance vascular constrictive remodeling by inactivating PPAR-alpha and -gamma in ECs and PPAR-gamma in SMCs.
- Expression of peroxisome proliferator-activated receptors (PPARs) in human urinary bladder carcinoma and growth inhibition by its agonists.
- This protein is a proangiogenic factor produced during ischemia and in conventional renal cell carcinoma.
- a link between metabolic disorders and hypoxia-induced angiogenesis.
- FIAF (angiopoietin-like 4 protein) may partially exert its function via a truncated form.
- Angptl4 transgenic mice displayed elevated plasma triglycerides and reduced postheparin plasma lipoprotein lipase(LPL). Recombinant Angptl4 inhibited mouse LPL and recombinant human LPL activity in vitro.
- Angptl4 plays important roles in lipoprotein lipase activity in both fed and fasted states
- Our hypothesis that variations of pO2 could exist between adipose tissue from anatomical origins was supported by staining of the hypoxic-induced angiopoietin ANGPTL4 depended on the location of fat.
- Resequencing of ANGPTL4 in a multiethnic population allowed analysis of the phenotypic effects.
- TGFbeta induction of Angptl4 in cancer cells that are about to enter the circulation enhances their subsequent retention in the lungs, but not in the bone.
- Microarray expression profile reveals the change of molecules involved in the synoikis-like hepatoma cells and our data indicated that ANGPTL4 contributed to anoikis resistance of hepatoma cells.
- common genetic variation within the ANGPTL4 gene may not play a major role in the development of prediabetic phenotypes in our white population.
- Hypoxia upregulates the expression of angiopoietin-like-4 in human articular chondrocytes: role of angiopoietin-like-4 in the expression of matrix metalloproteinases MMP1 and MMP3 and cartilage degradation
- triglyceride concentration differences among adipokine angiopoietin-like 4 (ANGPTL4[E40K]) A allele carriers and G allele homozygotes are maintained over time; degree of triglyceride increase was similar between groups and not modified by weight changes
- the 40K variant of ANGPTL4 appeared to confer reduced genetic risk for CHD.
- Although associated with an atheroprotective lipid profile, E40K was associated with increased CHD risk, suggesting Angptl4 influences parameters beyond lipid levels. T266M showed effects only under conditions of postprandial stress.
- The finding that ANGPTL3 and ANGPTL4 inhibit LPL activity through distinct mechanisms indicates that the two proteins play unique roles in modulation of lipid metabolism in vivo.
- ANGPTL4 is produced by human myotubes in response to long chain fatty acids via PPAR-delta.