|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for P2RY1(NM_002563.4) Search again
Product ID:
HQP012101
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
P2Y1, SARCC
Gene Description:
purinergic receptor P2Y1
Target Gene Accession:
NM_002563.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Summary
The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor functions as a receptor for extracellular ATP and ADP. In platelets binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and probably to platelet aggregation. [provided by RefSeq].
Gene References into function
- expression profile in human peripheral tissues and brain regions using PCR
- Inhibition of platelet P2Y12 and alpha2A receptor signaling by cGMP-dependent protein kinase.
- immunolocalization of P2Y1 and TPalpha receptors in platelets revealed that while present at the platelet surface, both receptors were abundantly represented inside the platelet - in membranes of alpha-granules and elements of the open-canalicular system
- Human keratinocytes express multiple P2Y-receptors: evidence for functional P2Y1, P2Y2, and P2Y4 receptors.
- the P2Y(1) purinoceptor and the P2Y(12)receptor appear to be involved in ADP-induced platelet shape change, an early phase of platelet activation that precedes aggregation
- Different purinergic receptors have different functional roles in human epidermis with P2Y1 and P2Y2 receptors controlling proliferation, while P2X5 and P2X7 receptors control early differentiation, terminal differentiation and death of keratinocytes.
- Non-melanoma skin cancers express functional purinergic receptors and that P2X7 receptor agonists significantly reduce cell numbers in vitro.
- Supports P2Y(12) as a drug target compared with P2Y(1).
- Whereas P2RY1 antagonism did not affect collagen or thrombin-induced thrombin generation, P2RY12 antagonism did decrease both, suggesting that P2RY12, but not P2RY1, is responsible for the potentiation of agonist-induced platelet procoagulant activity.
- Src kinase is activated through P2Y(1) but not P2Y(12)
- both P2Y12 and P2Y1 contribute to ADP-potentiation of platelet-derived microparticles generation induced by collagen
- ADP signaling through P2Y(1) may contribute to the initial stages of platelet adhesion and activation mediated by immobilized VWF, and through P2Y(12) to sustained thrombus formation.
- Basal activity of the P2Y(1) receptor is maintained by paracrine or autocrine release of receptor agonists.
- Results show that the two arginine residues (R333R334) in the carboxyl terminus of the human P2Y(1) ADP receptor are essential for G(q) coupling.
- The P2Y1 has an exclusive vascular distribution.
- Agonist-induced P2Y1 receptor desensitization was accompanied by its internalization in platelets and transfected cells.
- both P2Y(1) and P2Y(12) desensitize in human platelets; P2Y(12), but not P2Y(1), desensitization is mediated by GRKs
- Induction of the osmolyte permeability in Plasmodium-infected erythrocytes involves autocrine purinoceptor signaling.
- We conclude that lipid rafts play a significant role in the regulation of P2X1 but not P2Y1 receptors in human platelets and that a reserve of non-functional P2X1 receptors may exist.
- the A1622G dimorphism in P2Y1 is not associated with ADP-induced platelet activation/aggregation after treatment with clopidogrel
- Stimulation of ARO cells evidenced a major involvement of P2Y1 and P2Y2 receptors in controlling the Hsp90 activation. P2Y1 and P2Y2 resulted significantly upregulated in sample biopsies from different thyroid tumors.
- This study therefore is the first to reveal distinct roles for PKC isoforms in the regulation of platelet P2Y receptor function and trafficking.
- results suggest that the interaction of calmodulin with the P2Y1 C-terminal tail may regulate P2Y1-dependent platelet aggregation
- To determine if polymorphisms in P2X7 are associated with increased risk of TB. The 1513C allele increases susceptibility to extrapulmonary TB.
- Presence of the P2Y1 893CC genotype appears to confer an attenuated antiplatelet effect during aspirin treatment in healthy Chinese volunteers.
- oligomerization of the P2Y11 receptor with the P2Y1 receptor controls the internalization and ligand selectivity of the P2Y11 receptor
- These results highlight a role of P2Y(1)R in EGFR-dependent epithelial cell proliferation. P2Y(1)R could potentially mediate both trophic stimuli of basally released nucleotides and first-line mitogenic stimulation upon tissue damage.
- ADP promotes human endothelial cell migration by activating P2Y1 receptor-mediated MAPK pathways, possibly contributing to reendothelialization and angiogenesis after vascular injury
- analysis of constitutive and agonist-induced dimerizations of the P2Y1 receptor
- Olfactory nerve terminals release not only glutamate, but also ATP, which activates P2Y(1) receptors.
- The interaction between A(1) and P2Y(1) receptors may play an important role in the purinergic signaling cascade in astrocytes
- stress induces release of ATP, which in turn mediates Rho kinase activation through the P2Y1 receptor, resulting in the up-regulation of OPN [and] could play a significant role in alveolar bone resorption
- In intact human vascular smooth muscle the association of the P2Y(1)R to membrane rafts, highlighting the role of this microdomain in P2Y(1)R signaling.