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Validated All-in-One™ qPCR Primer for OGG1(NM_002542.5) Search again
Product ID:
HQP012021
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
HMMH, HOGG1, MUTM, OGH1
Gene Description:
8-oxoguanine DNA glycosylase
Target Gene Accession:
NM_002542.5(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined.
Gene References into function
- OGG1 plays an important role in cell survival from radiation-induced damage
- Expression of 8-oxoguanine DNA glycosylase is reduced and associated with neurofibrillary tangles in Alzheimer's disease brain.
- crystal structure of native hOgg1 refined to 2.15 A resolution that reveals a number of highly significant conformational changes on association with DNA that are clearly required for substrate recognition and specificity
- human OGG1 Cys/Cys genotype may confer a 2-fold increased risk of lung cancer
- hOGG1 Ser(326)Cys polymorphism and modification by environmental factors of stomach cancer risk in Chinese
- Human OGG1 undergoes serine phosphorylation and associates with the nuclear matrix and mitotic chromatin in vivo.
- These results suggest that hOGG1 may play an important role in the repair of 8-OH-dG adducts in the aerodigestive tract and that the hOGG1 Ser326Cys polymorphism plays an important role in risk for smoking- and alcohol-related orolaryngeal cancer
- may reduce oxygen mediated DNA damage in lung cells
- the association of OGG1 Ser326Cys polymorphism was limited for the risk of lung adenocarcinoma
- Inter-individual variation, seasonal variation and close correlation of OGG1 and ERCC1 mRNA levels in full blood
- Data show that overexpression of 8-oxoguanine DNA glycosylase/apurinic lyase (OGG1) causes enhanced repair of and increased resistance to oxidative damage to mtDNA.
- Aspartate-268 of OGG1 is a critical amino acid residue that plays a dual role, acting both as an N-terminal alpha-helix cap and as a critical residue for catalysis of both base excision and DNA strand cleavage by the enzyme.
- we report the structure of a trapped catalytic intermediate in DNA repair by human 8-oxoguanine DNA glycosylase
- Of the 18 cancer cell lines treated with oh(8)dG, 3 cell lines (H9, CEM-CM3, and Molt-4) were found to be committed to apoptosis, and all of these showed very low OGG1 activity and a marked increase in the concentration of oh(8)Gua in DNA.
- study showed that 8-oxoguanine DNA glycosylase 1 is the only glycosylase that incises 8-oxoadenine, when base-paired with cytosine in mitochondria and nuclei, but a different enzyme incises 8-oxoadenine when base-paired with guanine in the nucleus
- association of 8-oxoguanine glycosylase I polymorphism of Ser326Cys substitution with colon cancer risk and possible interaction with known environmental risk factors
- hOGG1 expression change during differentiation of hematopoietic stem cells for adaptation to new environments
- hOGG1 Ser326Cys polymorphism is unlikely to play a modifying role in individual susceptibility to breast cancer among Asian women.
- OGG1 and MYH function as suppressors for G:C to T:A transversions by 8OHG but not by BPDE in human cells.
- Significant age-dependent decrease in hOgg1 activity in lymphocytes. Significantly reduced activity was also shown in with Cysteine/Cysteine genotypes. The genders of the subjects were not shown to be associated.
- hOGG1 protein turnover may be sensitive to intracellular redox changes
- data presented support a model by which X-ray repair cross complementing protein 1 (XRCC1) will pass on the DNA intermediate from DNA glycosylase hOGG1 to the endonuclease APE1
- hOGG1 may have a role in the repair of 8-OH-dG adducts in prostate tissue and hOGG1 Ser326Cys polymorphism is associated with prostate cancer risk.
- findings indicate that loss of hOGG1 expression may have a role in development or progression of clear cell renal cell carcinoma.
- OGG1 is upregulated by NFYA following administration of DNA-alkylating agents
- hOgg1 mutant proteins with a substitution of H270A or F319A are members of a new type of hOgg1 that is deficient in DNA glycosylase but proficient in AP lyase
- hOGG1 polymorphism is associated with lung cancer risk and is linked to exposure to tobacco smoke
- Finds no association between the hOGG1 (Ser326Cys) polymorphism and squamous cell carcinoma of the head and neck.
- Because there is an increased incidence of lung and small intestine cancer in Myh(-/-)/Ogg1(-/-) mice, these findings support a causal role for unrepaired oxidized DNA bases in cancer development.
- Single nucleotide polymorphism may play a role in the pathogenesis of lung cancer in this population, particularly among heavily exposed women.
- OGG1 and Nei-like endonuclease (NEI1), DNA glycosylases which do not stably interact, stimulate 8-oxoguanine repair by a collaboration that is possible because of higher abasic (AP) site affinity and stronger AP lyase activity of NEI1 relative to OGG1.
- OGG1 R154H may function as a low/moderate-penetrance modifier for colorectal cancer development
- The mitochondrial beta-Ogg1 isoform lacks 8-oxoguanine DNA glycosylase activity.
- hOGG1 Ser326Cys polymorphism is associated with gastric cancer
- hOgg1 mainly operates as a monofunctional glycosylase under physiological concentrations of magnesium
- human OGG1 is regulated by cadmium through suppression of Sp1 activity
- Relocalization of hOGG1 to the nucleoli during the S-phase in cells expressing the hOGG1-Cys326 polymorphic variant.
- X ray structure of human oxoG repair protein, 8-oxoguanine DNA glycosylase I (hOGG1), in the act of interrogating normal DNA
- OGG1 targeted to mitochondria reduces the activation of caspase-9
- Higher expression levels of mitochondrial isoforms of OGG1 enzymes in the substantia nigra (SN) in cases of PD. Furthermore, Western blot analysis revealed high OGG1 levels in the SN of the patients with PD.
- Under-expression of hOGG1 mRNA and hOgg1 protein was associated with a decrease in mitochondrial DNA repair in response to oxidative damaging agents.
- Allelic loss of the DNA repair gene OGG1 against oxidative damage ii associated with esophageal squamous cell carcinoma
- Tat may play a role in maintenance of the genetic integrity of the proviral and host cellular genomes by up-regulating OGG1 as a feed-forward mechanism
- Phosphorylation of human oxoguanine DNA glycosylase (alpha-OGG1) affects its catalytic activity.
- A base excision repair enzyme involved in the 8-oxoguanine (oxoG) repair pathway.
- Kinetics of 8-oxo-7,8-dihydroguanine recognition and cleavage by OGG1.
- observations demonstrate, for the first time, that reduction of hyperoxic toxicity by base excision DNA repair proteins may be involved with MAPK activity, thereby impacting cell survival
- OGG1 plays role in the processing of radiation damages
- Meta-analyses found increased lung cancer risk among subjects carrying the OGG1 Cys/Cys genotype.
- Loss of p53 function can lead to decreased hOgg1 repair activity.
- The OGG1 Ser326Cys gene polymorphism is associated with decreased insulin sensitivity in subjects with normal glucose tolerance.
- OGG1 polymorphism with cigarette smoking is associated with head and neck squamous cell carcinoma
- OGG1 is acetylated in vivo predominantly at Lys338/Lys341, we propose a novel regulatory function of OGG1 acetylation in repair of its substrates in oxidatively stressed cells.
- The data suggest that the OGG1 Ser326Cys polymorphism may be associated with tooth loss.
- Polymorphic S326C OGG1 glycosylase has altered substrate specificity, lack of stimulation by AP-endonuclease 1 and anomalous DNA binding conformation.
- Overexpression in mitochondria decreased free fatty acid-induced inhibition of ATP production and protects cells from apoptosis.
- DNA glycosylases locate lesion bases by a massively redundant search in which the enzyme selectively binds 8-oxoguanine under kinetic control.
- The use of hOGG1 in the modified comet assay offers a useful alternative to FPG and is apparently more specific for 8-oxoguanine and methyl-fapy-guanine.
- These results indicate that OGG1-326Cys functions as a risk allele for lung ADC development.
- The finding of decreased expression levels for hOGG1 and XRCC1 in gamma-irradiated lymphocytes has not been reported elsewhere.
- cadmium inhibits hOGG1 activity mainly by indirect oxidation of critical cysteine residues
- The hOGG1 mRNA expression was greater in depressive patients with acute leukemia than in the controls.
- the OGG1 Ser326Cys polymorphism does not have a role in development of lung cancer
- The X-ray structure of trapped hOGG1 complex sampling an undamaged G:C base pair located adjacent to an oxoG:C base pair reveals that the presence of the 8-oxoguanine drastically changes the local conformation of the extruded G.
- The R229Q hOGG1 mutation fails to protect cells from oxidative damage. Such mutations in cancer may be more detrimental to cellular survival when present in the mitochondria than in the nucleus.
- We were not able to demonstrate an association between the Hemicentin-1, hOgg1, and E-selectin SNPs and age-related macular degeneration development in the currently available cases and controls.
- three pre-excision steps (i) initial encounter with eversion of the damaged base, (ii) insertion of several enzyme residues into DNA, and (iii) enzyme isomerization to the catalytically competent form
- characterization of human 8-oxoguanine DNA glycosylase variants bearing active site mutations
- 8-oxoguanine-DNA glycosylase interaction with Ap endonuclease.
- Polymorphisms in OGG1 enzyme is associated with Helicobacter pylori infections in gastric cancer
- reactive oxygen species are the signals for the relocalisation of hOGG1 to nuclear speckles
- The genetic polymorphism in OGG1 1245C/G is not associated with susceptibility to COPD in a southern Chinese population of Han nationality.
- Quantitative features of recognition of specific DNA by 8-oxoguanine-DNA glycosylase are analyzed.
- Present results suggest that the Ser326Cys polymorphism of the hOGG1 gene is not an independent risk factor for sAD.
- Two novel mutations of MYH and a novel OGG1 polymorphism seemed to be associated with multiple colorectal adenomas in Korean families.
- Over-expression in mitochondria may promote mitochondrial DNA damage by creating an imbalance in the base excision repair pathway and sensitize cancer cells to cisplatin.
- Taken together, our results indicate that hOGG1 is a direct target of TAp63gamma, suggesting a role for TAp63gamma in oxidative damage and repair.
- These results suggest that hOGG1 Ser326Cys polymorphism is associated with gallbladder cancer risk.
- Nuclear extracts derived from patients with Alzheimer's disease with OGG1 mutations exhibited greatly reduced 8-oxoG glycosylase activity.
- study showed significant difference in the genotype allele frequency of OGG1 S326C in Saudis compared to Chubese and Japanese populations
- Present results suggest a possible involvement of the hOGG1 Ser326Cys polymorphism in sALS pathogenesis.
- The substrate specificity of two similar enzymes, MutM of E. coli and OGG1 of H. sapiens, is reported.
- Expression of R229Q OGG1 sensitized KG-1 cells to killing by menadione and 8-hydroxydeoxyguanosine, but not ionizing radiation; the R229Q OGG1 allele may be a novel marker for cancer susceptibility.
- hOGG1 Ser326Cys polymorphism is not a major genetic risk factor for colorectal cancer
- Thai women with a certain XRCC1 diplotype or homozygous for two or three variant alleles of XRCC1, OGG1, and APEX1 are likely to have an increased susceptibility to breast cancer.
- Ser(326)Cys variant of OGG1 may confer risk for development of angiomyolipomas by increasing oxidative DNA damage.
- Although p53 is a major modulator of apoptosis, hOGG1 also plays a pivotal role in protecting cells against the H(2)O(2)-induced apoptosis at the upstream of the p53-dependent pathway.
- Evidence that the combination of different DNA repair genes (hOGG1, XRCC1 and XRCC3) and their interaction with environmental genotoxic agents may modulate micronuclei induction.
- Tuberin regulates a specific DNA repair enzyme, OGG1. This regulation may be important in the pathogenesis of kidney tumors in patients with tuberous sclerosis complex.
- The superb activity of the 8-oxoguanine glycosylases Fpg, hOGG1, and Ntg1 toward removal of FapyG and FapyA in vitro suggests that these enzymes may act upon these oxidized lesions in vivo.
- OGG1 mRNA levels should be regarded as a biomarker of exposure to oxidative stress with induction of DNA rather than a marker of inborn DNA repair capacity.
- APEX1 serves to improve specificity of OGG1 for its biologically relevant substrates
- MTS-hOGG1 plays an important protective role against 2-ME-mediated mitochondrial damage by blocking apoptosis induced through the Fas pathway.
- It is indicated that the hOGG1 codon 326 and XRCC1 codon 399 polymorphisms are risk factors of bladder cancer.
- LOH at hOGG1 gene is associated with melanoma in situ and atypical melanocytic hyperplasia, suggesting that the hOGG1 gene may play a role in melanocytic tumor progression.
- No significant association between the hOGG1 Ser326Cys polymorphism and the risk of esophageal adenocarcinoma, Barrett's esophagus or reflux esophagitis.
- OGG1 1245C-->G polymorphism was common in both GC and IMA, but very rare in controls, and correlated more closely with 8-OHdG levels than do H. pylori infection or cagA status.
- The associated polymorphism is located in intron 9 and in the haplotype block encompassing the alternatively spliced exons of the geneThe NTNG1 gene is associated with schizophrenia in the Japanese.
- The study demonstrated the positive association between the polymorphisms of XRCC1 and hOGG1 genes and laryngeal carcinoma.
- The results suggest a link between the 8-oxoG lesion and UVA-induced mutagenesis. We propose that hOGG1 has a role in maintaining genomic stability in mammalian cells after oxidative stress.
- Deletion of hOGG1 gene occurs commonly in papillary and clear cell-renal cell carcinoma but not in renal cortical papillary adenoma.
- Our results indicate that weaker expression of the nuclear form of hOGG1 enzyme in the basal cells of the epidermis may lead to a lack of DNA repair in these cells and therefore accumulation of UVA-induced oxidative DNA mutations.
- Individuals carrying the hOGG1 Cys/Cys genotype did not have significantly increased risk of lung cancer.
- This study showed that green tea intervention might be effective in decreasing DNA damage in the subgroup of smokers who are GSTM1 positive regardless of their hOGG1 genotype.
- polymorphic variants of the hOGG1 gene are not independent risk factors for Alzheimer's disease
- Data show that processive cleavage by OGG1 does not correlate with their substrate specificity and under nearly physiological salt conditions may be replaced with the distributive mode of action.
- These results suggest that the single nucleotide polymorphisms of OGG1 and MTH1 may be cause of 8-hydroxy-2'-deoxyguanosine accumulation in the gastric mucosa.
- Heterozygosis for the genes hOGG was found to be a protective factor to malignant tumors in exposed person
- Single nucleotide polymorphisms (SNPs) in hMTH1, hOGG1 and hMYH genes are associated with risk of chronic benzene poisoning.
- The p21 codon 31*C- and DRD2 codon 313*T-related genotypes/alleles, but not XRCC1 codon 399, hOGG1 codon 326, and DRD1-48 polymorphisms, are correlated with the presence of leiomyoma.