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Validated All-in-One™ qPCR Primer for NPAS2(NM_002518.3) Search again
Powered by BiozBy default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. A similar mouse protein may play a regulatory role in the acquisition of specific types of memory. It also may function as a part of a molecular clock operative in the mammalian forebrain. [provided by RefSeq].
Gene References into function
- A significant difference between patients with seasonal affective disorder (SAD) and controls is found for NPAS2 protein (471 Leu/Ser) indicating a recessive effect of the leucine allele on SAD susceptibility.
- the CLOCK(NPAS2)/BMAL1 complex is post-translationally regulated by cry1 and cry2
- Our results demonstrate a robust association of the variant Thr genotypes (Ala/Thr and Thr/Thr) with reduced risk of non-Hodgkin's lymphoma.
- With autisitc disorder, haplotype analysis of two-marker haplotypes showed that in npas2 40 out of the 136 possible two-marker combinations were significant, with the best result between markers rs1811399 and rs2117714.
- a role of the circadian gene NPAS2 in human breast cancer, suggesting that genetic variations in circadian genes might be a novel panel of biomarkers for breast cancer risk.
- variations associated with seasonal affective disorder
- knockdown of NPAS2 significantly represses the expression of several cell cycle and DNA repair genes in breast and colorectal neoplasms.
- Variations in circadian genes are associated with serum levels of androgens and IGF markers, particularly NPAS2 rs2305160:G>A(Ala394Thr).