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Validated All-in-One™ qPCR Primer for NOS2(NM_000625.3) Search again
Product ID:
HQP011866
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
HEP-NOS, INOS, NOS, NOS2A
Gene Description:
nitric oxide synthase 2
Target Gene Accession:
NM_000625.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq].
Gene References into function
- The specific NOS2 inhibitor, 1400W, sensitizes HepG2 cells to genotoxic, oxidative, xenobiotic, and endoplasmic reticulum stresses
- Inos enzyme overexpressioin is an early event in esophageal carcinogenesis and useful biomarkers for early detection.
- Human renal epithelial cells express iNOS in response to cytokines but not bacteria
- The human alveolar type II epithelium-like cell line A549 expresses nitric oxide synthase type 2 (NOS2)
- Immunohistochemical expression of iNOS in colorectal carcinoma
- Increased expression of iNOS may be responsible for gastric carcinogenesis and tumor aggressiveness of gastric cancer and correlates significantly with lymph node metastasis in northern China.
- demonstrated that the iNOS gene was actively transcribed in cutaneous leishmaniasis lesions
- Inducible nitric oxide synthase is expressed in normal human melanocytes but not in melanoma cells
- epithelial iNOS binds to EPB50 which directs vectorial nitric oxide output
- allelic polymorphism of the inducible nitric oxide synthase (iNOS) gene associated with retinopathy in an Asian Indian population
- enzyme expressed in septic patients is nitrated on selected tyrosine residues; implications for enzymic activity
- expression of inducible nitric oxide synthase in skin lesions of patients with cutaneous leishmaniasis
- Data show that prostacyclin receptor mediated increases in cAMP play a role in enhancing LPS/IFN-gamma-induced iNOS expression in human monocytes/macrophages and may contribute to the increased production of NO during peritonitis.
- expression is increased in malaria, thus producing more nitric oxide
- inducible nitric oxide synthase promoter polymorphism in rheumatoid arthritis
- iNOS is induced by HIV-1 tat in astrocytes and may play a role in HIV-associated dementia
- expressions of iNOS and VEGF may serve as indexes for evaluating staging of gastric carcinoma and forecasting its risk of metastasis
- iNOS is subject to ubiquitination and ubiquitination is required for its degradation.
- EGF protects against oxidative disruption of the intestinal barrier by stabilizing the cytoskeleton in large part through the activation of PKC-zeta and downregulation of iNOS
- These results suggest that IL-15 is a potent regulator of iNOS expression by HGEC and involved in innate immunity in the mucosal epithelium.
- Data demonstrate that the PI3-kinase signaling pathway is involved in basal trophoblast motility and that both mitogen-activated protein kinase and PI3-kinase signaling pathways are important in HGF-stimulated motility and iNOS expression.
- Synovial fluid leucocytes, in particular granulocytes, express iNOS and may thus contribute to intra-articular NO production in arthritis.
- iNOS may not correlate with cancer cell-proliferative activity or apoptosis
- role of NF-kappa B-repressing factor (NRF) in basal repression of the hiNOS gene
- strong upregulation of iNOS might contribute to inflammatory processes in fulminant hepatic failure
- Activation might be associated with malignant potential of epithelial odontogenic tumors
- Association analysis of five polymorphic microsatellite markers in cluster headache (CH) patients reveals that genetic variations in NOS2A do not contribute greatly to CH susceptibility.
- hepatic inducible nitric oxide synthase staining was significantly more intense in patients with chronic viral hepatitis; these results suggest that inducible nitric oxide synthase may have a critical role in the pathogenesis of chronic viral hepatitis
- NOS2 promoter -1173 C-->T single nucleotide polymorphism is associated with protection against cerebral malaria and severe malarial anaemia
- Increased intrahepatic iNOS expression and nitrotyrosine accumulation in biliary cirrhosis and autoimmune hepatitis, related to histological severity of liver disease.
- nitric oxide synthases(inducible, brain and endothelial) were expressed in human pregnant term uterus and did not change in the myometrium during labor
- A positive correlation was found between the positive expression of iNOS mRNA and invasive growth as well as neck lymph node metastatsis of squamous cell carcinoma of tongue.
- Data show that nitric oxide produced by inducible nitric oxide synthase (iNOS) increases the activity of cyclooxygenase-2 (COX-2) pathways in head and neck squamous cell carcinomas, and this effect is probably mediated by endocellular cGMP.
- inducible nitric oxide synthase-cyclooxygenase 2 interactions are involved in tumor cell angiogenesis and migration
- Upregulation of iNOS in myelodysplastic syndromes has no significant correlation with apoptosis.
- Immunolocalization of inducible nitric oxide synthase in gingiva in localized juvenile periodontitis patients. iNOS in gingivitis. Macrophages expressed high levels of iNOS.
- Altered genetic control of NOS2 transcription may be a risk factor for SLE among African-American females.
- study demonstrate that expression of the MDA-7 tumor suppressor can negatively regulate iNOS expression in malignant melanoma cell lines
- A single haplotype, uniquely defined by the NOS2A-1659T allele, was associated with cerebral malaria by a transmission disequilibrium .
- upregulation of hepatic iNOS, associated with peroxisomal localization, is an early molecular early molecular response to hemorrhagic shock
- Studies of binding of KLF6 to the human iNOS promoter reveal CACCC binding sites and transactivation of iNOS promoter in diverse pathophysiological conditions: NaCN-induced hypoxia, heat shock, serum starvation, and PMA/ionophore stimulation
- Expression in human colorectal cancer correlates with tumor angiogenesis.
- findings suggest that upregulation of iNOS expression for local NO production in bone marrow may contribute in part to the pathogenesis of aplastic anemia
- results suggest that LTA might activate PC-PLC and PI-PLC to induce PKC activation, which in turn initiates NF-kappaB activation, and finally induces iNOS expression and NO release in RAW 264.7 macrophages
- A significant increase in calcium/calmodulin-independent NOS activity (iNOS) and iNOS mRNA expression was found in the medulla of obstructed kidneys.
- Adenoviral-mediated overexpression of human iNOS causes increased superoxide production in carotid arteries from cholesterol- but not chow-fed rabbits. It is associated with reduced endothelium-dependent and independent vessel relaxation in these animals
- results do not support any association of iNOS gene polymorphism to the development of coronary artery lesion in a Japanese population
- influence of promoter polymorphism of NOS2A gene on susceptibility and outcome was studied in 140 multiple sclerosis Spanish patients; no association was found between susceptibility, course or outcome of the disease, and NOS2A polymorphisms
- rate of intramolecular electron transfer between the two flavins in the iNOS flavin domain was faster than that of the nNOS flavin domain.
- a physical interaction between c-Fos and STAT-1 participates in NOS2 gene transcriptional activation in lung epithelium
- evidence of undetectable levels of TNF-alpha and NO in infants with congenital hypothyroidism
- The transition from hyperplastic ACF to dysplastic ACF may be a crucial step in the ACF-adenoma-carcinoma sequence, in which iNOS plays an important role by regulating tumor cell apoptosis, proliferation and angiogenesis.
- Results describe the expression of nitric oxide synthases 1, 2, and 3 in patients with chronic obstructive pulmonary disease, suggesting their involvement in muscle dysfunction in this disease.
- Immunocytochemical localization in oral mucosa and lymph nodes of patients with paracoccidioidomycosis
- The results suggest that activation and nuclear localization of EGFR may be needed for induction of NOS-2 in response to elevated intraocular pressure in glaucomatous optic neuropathy.
- Data describe the correlation between inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 activities and p53 gene status in head and neck squamous cell carcinomas in vivo and in vitro.
- NF-kappaB activation may play a role in protecting gastric epithelial cells from H. pylori-induced apoptosis by upregulating endogenous inducible nitric oxide synthase.
- NO generation from iNOS in the malignant epithelium and from eNOS in tumor stroma play a important role in tumor angiogenesis in bladder cancer
- potential mechanism for reduced NOS2 expression in cystic fibrosis is diminished signal transducer and activator of transcription-1 (STAT1) activity, possibly due to an increase in expression of protein inhibitor of activated STAT1 (PIAS1).
- iNOS transcription involves CCAAT-Enhancer-Binding Protein-beta binding to a promoter response element in liver cells.
- Haplotypic relationship between SNP and microsatellite markers at the NOS2A locus was studied in two populations.
- induction of iNOS splice variants in chronic lymphocytic leukemia B cells leads to the activation of a caspase-3-dependent apoptotic pathway
- mechanisms of in vivo iNOS dimer formation
- Both eNOS and iNOS mRNA expression in placental tissues in labor were significantly higher than those in the amnion, chorion laeve, decidua vera and myometrium.
- In contrast to unseparated blood cells, NOS II was demonstrable both in isolated blood neutrophils and exudated cells.
- Hepatitis C virus core protein transactivates iNOS gene promoter through NF-kappaB activation
- Lymphocytic infiltration and iNOS expression and activity were detected in duodenal and colonic mucosa from patients with ankylosing spondylitis. May indicate inflammatory process in small intestine and colon of patients with ankylosing spondylitis.
- The FMN/FMNH couple is ~100 mV in all 3 isoforms and unlikely to be catalytically competent; the other three flavin couples are around -250 mV. Reduction of the flavins by the pyridine nucleotide couple at -325 mV is thermodynamically favorable.
- Data suggest that expression of inducible nitric oxide synthase in circulating leukocytes and endothelial cells correlates with the severity of coronary arterial wall injury and the progression of lesions in patients with acute Kawasaki disease.
- iNOS is suggested to be a significant molecule for renal cell carcinoma to acquire not only hypoxic adaptation but also the ability to invade into veins and form tumor thrombi.
- The expression of iNOS is related to the biological behaviors of primary gallbladder carcinoma
- Data show that relaxin potentiates the expression of inducible nitric oxide synthase by endothelial cells from human umbilical vein
- Healthy human dental pulp tissue failed to exhibit any iNOS; however, acute inflammation enhanced the mRNA and protein levels of iNOS, mainly in the leukocytes.
- autocrine activation of NOS2 is defective in cystic fibrosis cells, but IFN-gamma induction of antiviral host defense is intact.
- Results describe the relationship between the expression of vascular endothelial growth factor and inducible nitric oxide synthase in gastric cancer.
- JNK signaling plays an important role in the inhibitory effects of cAMP on IL-1beta+IFNgamma-induced iNOS gene expression in cultured hepatocytes
- This study results provide strong evidence for linkage and association to a new candidate disease gene on chromosome 17q11 in MS and suggest that variation within NOS2A or a nearby locus contributes to disease susceptibility.
- Exercise during head-down-tilt bed rest altered NOS 2/caveolin 3 co-immunostaining patterns of subsarcolemmal focal accumulations in vastus lateralis or soleus myofibers, which suggests reorganization of sarcolemmal microdomains.
- Nitric oxixde generated by iNOS predominantly inhibits the growth of tumor cells in their free form, but enhances the growth of solid tumors.
- iNOS and COX-2 may play a synergistic role in the pathogenesis of gastric MALT lymphoma.
- half of the cases expressed iNOS and COX-2, but these two enzymes do not seem to be the key step in angiogenesis or carcinogenesis of pancreatic adenocarcinomas.
- Constitutive NOS activities are responsible for E2-induced NO production in neuroblastoma cells. Differential activation of NOS isoforms in these cells occurs in response to different treatments.
- Chronic H pylori infection induces iNOS expression and subsequent DNA damage as well as enhances anti-apoptosis signal transduction.
- activity in peripheral blood mononuclear cells (PBMCs) is significantly elevated in children with Plasmodium falciparum malarial anemia and inversely associated with hemoglobin levels.
- The coupled induction of iNOS and p53 upregulation in intrinsic renal cells of IgA nephropathy may be linked with both pro- and anti-apoptotic activities
- fivefold upregulation of interferon gamma (IFN-gamma) gene expression. Inducible, but not constitutive NO-synthase gene expression was upregulated fivefold in samples from rejecting patients suggesting a local induction of NO in response to immune events
- Ultraviolet beta rays induce iNOS mRNA and enzyme activity in skin endothelial cells
- iNOS Ser(608)Leu allele may be a potential determinant of susceptibility to cigarette -alcohol induced gastric cancer
- Expression of inducible nitric oxide synthase is negatively associated with human breast cancer progression
- Endothelial nitric oxide synthase gene polymorphism is associated with endothelial dysfunction in healthy young men.
- down-regulation by LPA of cytokine-induced increases in NOS2 activity is likely to involve a Rho-dependent signaling pathway
- analysis of the cellular posttranslational mechanisms of iNOS
- Inactivation of antioncogene p53 and overexpression of iNOS might play a synergetic role in the process of carcinogenesis of human gastric carcinoma.
- The inducible nitric oxide synthase Expression is generally accompanied by a parallel upregulation in l-arginine transport which is dependent, at least in part, on the synthesis of new carrier proteins.
- The inducible nitric oxide synthase expression and activity were found in almost 50% of bladder cancer patients, in both BT and in NT.
- activation of AP-1 is involved only during the low level of iNOS induction by IL-1beta but not during the high level of induction by IL-IF. Activation of NF-kappaB and C/EBPbeta was involved in the induction of iNOS by IL-1beta as well as by IL-IF.
- important role in the early stage of colorectal carcinogenesis
- Higher expression of iNOS is associated with neoplasm invasiveness of retinoblastoma
- The relationship of NOS gene variants to Fabry disease, hypertension and coronary disease was studied.
- Suggest that HIF-1alpha induces VEGF/ET-1/iNOS during gastric ulcer healing.
- NO produced by iNOS may play a stronger role in promoting gastric adenocarcinoma growth than in suppressing its growth
- Significant differences in the NOS2A promoter polymorphism allele and genotype frequency between Henoch-Schonlein Purppura(HSP)patients and controls suggest a potential role for this gene in the susceptibility to HSP and in the development of nephritis
- over expression of iNOS might be associated with tumor progression by stimulating angiogenesis and suppressing immune responses in gastric carcinoma
- A mutational analysis was performed on 30 samples to detect polymorphisms in the NOS2 promoter region that contains important NF-kappaB sites and three point mutations were identified in this region
- The lack of stimulating signals may be a reason for negative iNOS detection and negligible apoptotic rate in parathryoid adenoma
- TIAR seems to be involved in the post-transcriptional regulation of human iNOS expression
- IL-1Ra, Bcl-2, and iNOS are upregulated and counter the proinflammatory and proapoptotic effects of ischemia-reperfusion
- The effect of a soluble Abeta peptide, Abeta(1-40), on the expression of NOS-2 in astrocytes was determined.
- The expression of iNOS was related with tumor angiogenesis.
- iNOS seems to be a major instigator of beta-amyloid deposition and disease progression in mice with Alzheimer's-like disease resulting from transgenic expression of mutant human beta-amyloid precursor protein and presenilin-1.
- potential implication for NOS2A TAAA gene polymorphism in giant cell arteritis susceptibility
- acute alcohol intoxication is accompanied by changes in iNOS enzyme expression in certain brain structures
- These results suggest that iNOS-mediated nitrative stress contributes to development of oral carcinogenesis from leukoplakia through DNA damage as well as oxidative stress.
- iNOS has a role in L. tropica especially during the early stages of the infection
- e-NOS and i-NOS are weakly expressed in the MCF-7 cell line, but are stimulated differently.The MCF-7 cell may contain both a constitutive NOS and an inducible NOS.
- Reishi inhibits NOS2 expression in macrophages.
- iNOS and cyclooxygenase-2 play an important role in the apoptosis induction in trophoblasts of human fetal membrane tissues
- The observed decrease in pulmonary expression of iNOS in patients with CDH suggests a potential role in the pathogenesis of pulmonary hypertension in newborns with congenital diaphragmatic hernia.
- evidence presented that PARP-1 is a novel trans-activator of the iNOS promotor in mesengial cells and that its binding to a specific cis-element of the iNOS promotor appears to be regulated by the end product NO in a feedback inhibition circuit
- ability of iNOS to predict outcomes for these patients may be independent of other known prognostic factors, providing a new molecular marker with significant potential for clinical uti
- palmitoylation of inducible NOS at position 3 exquisitely determines its transit along the secretory pathway following a route that cannot be mimicked by a surrogate myristoylation or by a palmitate at position 2.
- Expression of iNOS in serous and low-grade carcinomas was significantly higher than that in nonserous and high-grade carcinomas
- mifepristone stimulates the release of NO and the expression of iNOS in cervical cells of women in early pregnancy
- Data suggest that human-specific release of nanomolar levels of nitrite may result from differences between human and mouse NOS2 genes, which may program different degrees of nitric oxide responses to inflammatory signals in humans than in mice.
- Polymorphisms in NOS2 gene are associated with transcriptional activity of NOS2 gene and with susceptibility to systemic sclerosis-related pulmonary hypertension.
- Results suggest that P. aeruginosa type III secretion system not only accounts for higher expression of Fas and release of FasL but also leads to overproduction of NO and to a NO/iNOS-dependent up-regulation of Fas-FasL proteins.
- This study support NOS2A as a genetic risk factor in PD, potentially by influencing AAO and by modifying the inverse association between PD and smoking.
- NOS overexpression stimulates the transcription and translation of a range of extracellular matrix genes important to the structure of connective tissues such as tendons
- These findings substantiate the hypothesis of transitory delayed inflammation, as suggested by the animal model, and suggest the possibility of using therapeutic approaches to target NF-kappaB transcription in the treatment of migraine.
- This study reveals the capacity of B-cell neoplasms to express iNOS in situ. In conclusion, our study revealed that there is a positive relation between iNOS expression and apoptosis (p = 0.032 spearman correlation).
- Over-expression of iNOS and HIF-1alpha in colorectal carcinoma is correlated with the biological character microvessel density.
- PTB is involved in the post-transcriptional regulation of INOS.
- current findings provide evidence for expression of all 3 of the NOS isoforms at all of the airway levels studied in the developing lung and in all of the levels studied of the developing vascular tree from 22 weeks to term
- These results suggest that iNOS plays a pivotal role in the endogenous production of NO under the basal condition, which is involved in the activity of the inwardly rectifying K(+) channel in cultured human proximal tubule cells.
- Astrocytes are a source of iNOS and may play a role in the evolution of pediatric brain injury days after the initial insult.
- Our study did not demonstrate an association between iNOS gene polymorphisms and diabetic nephropathy.
- TNF-induced activation of pro-inflammatory signaling in EC leading to enhanced expression of iNOS and NO production was dependent on Syk.
- The iNOS C150T polymorphism is associated with the risk of H pylori-related gastric cancer in a Japanese population, and it may play an important role in increasing the risk of gastric cancer in Asian countires with the highest rates of gastric cancer.
- NOS2A is not a major susceptibility locus in a relatively young sample population of 340 German patients with Parkinson disease.
- A mutation of iNOS Ser608Leu is associated with protection against silicosis and against severity of silicosis in Chinese iron miners exposed to silica dust.
- an oxidative DNA lesion, occurred to a much greater extent in Malignant fibrous histiocytoma (MFH) tissue specimens from deceased patients than in live patients. iNOS, NF-kappaB and COX-2 were colocalized with 8-nitroguanine in MFH tissues
- negative correlation between blood pressure and iNOS mRNA with intermittant, but not chronic hypoxia, may suggest differential modulation of the hemodynamic response to the 2 exposure patterns
- Extracellular SOD, endothelial NOS, and inducible NOS gene polymorphisms do not constitute a risk factor for developing BD (Behcet disease) in Japan.
- The results suggest that JNK regulates human iNOS expression by stabilizing iNOS mRNA possibly by a tristetraprolin -dependent mechanism.
- No association of NOS-2 and cyclooxygenase -2 polymorphisms with cervical cancer is found in Korean women.
- Differences in eNOS and iNOS mRNA expression at the adipose tissue level may have a limited effect on lipolysis and tissue perfusion.
- Results suggest that a polymorphism in the NOS2A gene influences the susceptibility to tuberculosis and suggests a role for NOS2A in the pathogenesis of mycobacterial infection.
- findings suggest that the polymorphic (CCTTT)n repeat in the iNOS promoter region might be involved in the development of urothelial carcinoma, especially in those who have ever smoked
- Peroxisome proliferator-activated receptor alpha/gamma ligands can down-regulate iNOS DNA methylation
- iNOS protein was detectable in peripheral-blood eEPCs, but not in cord-blood eEPCs and HUVECs; iNOS mRNA was more concentrated in peripheral-blood eEPCs than in cord-blood eEPCs and HUVECs
- The mechanisms by which the C-terminal four amino acids of inducible nitric oxide synthase (NOS2) interact with proteins that contain PDZ (PSD-95/DLG/ZO-1) domains resulting in the translocation of NOS2 to the cellular apical domain, is analyzed.
- all liver samples from patients with chronic hepatitis B and Hepatitis C had marked iNOS expression and a correlation between iNOS and severity of disease was detected
- Nitric oxide synthase induction has a role in cytotoxic nitrogen-related oxidant formation in conjunctival epithelium of dry eye
- iNOS protein seems to be associated with extracapsular spread and invasion in head and neck cancer.
- The levels of intralesional expression of mRNA of tumor necrosis factor-alpha, interferon, interleukin-10, RANTES, and indoleamine-2,3-dioxygenase in Mediterranean spotted fever are reported; levels of inducible nitric oxide synthase are also reported.
- increased expression of NOS2 in bronchial tissue & smooth muscle cells in COPD patients; upregulation of NOS2 in COPD is involved in airway tone regulation & functional airflow limitation; increased arginase activity is involved in airway sensitivity
- iNOS staining was not demonstrated in normal oral epithelium. In oral epithelial dysplasia, staining was seen in all cases (100%) in the basal layers of the epithelium and in 30% of cases it extended into the parabasal compartments as well.
- COX-2 and iNOS was significantly higher in chronic fatigue syndrome patients than in normal controls.
- NOS2 regulates cytokine-induced S-nitrosylation of p65, resulting in decreased NF-kappaB binding to the NOS2 promoter, thereby inhibiting further NOS2 expression.
- Study shows iNOS activity is acutely up-regulated by activation of the B1-kinin receptor in human endothelial cells or transfected HEK293 cells to generate 2.5-5-fold higher NO than that stimulated by Arg alone.
- Endogenous p53 mutations at CpG dinucleotides provide further evidence for a molecular link between chronic inflammation and esophageal malignancy.
- In conclusion, we found that the NOS2A (CCTTT)(14) allele was detected more frequently in the control group than in the RRMS patients, thus confirming the scientific interest on this marker.
- Toll-like receptors 2 and 4 amplify iNOS induction in gastric mucosa infected with Helicobacter pylori which contain lipopolysaccharide as a pathogen.
- rapid effects of pressure on iNOS may have important implications in the obstructed kidney
- individuals with impaired glucose homeostasis have reduced skeletal muscle nNOSmicro protein content
- the up-regulation of TCF11/MafG binding could be suppressed by overexpression of the TGF-beta inhibitor Smad7, and a small interfering RNA to TCF11 blocked the suppression of iNOS by TGF-beta.
- These findings demonstrate that c-Jun/AP-1 is hepatoprotective during acute hepatitis by regulating nos2/nitric oxide expression and thus functionally antagonizes the cell death-promoting functions of JNK.
- Allele (CCTTT) 13 is related to higher ulcerative colitis risk (p = 0.001; odds ratio [OR] [95% confidence interval, CI] = 1.64 [1.20-2.23]).
- It was seen that NOS2A polymorphism might play a role in the development of pre-eclampsia.
- NOS2 was not altered during tourniquet-induced ischemia/reperfusion.
- COX-2 has the ability to enhance iNOS-induced cPLA(2)alpha S-nitrosylation and maximal PG synthesis is achieved by the synergistic interaction among iNOS, cPLA(2)alpha, and COX-2
- PKCzeta is involved in regulation of IL-1beta-induced NF-kappaB signaling in human osteoarthritis chondrocytes, which regulates downstream expression of ADAMTS-4 and NOS2 via NF-kappaB.
- Glucosamine sulfate reverted IL-1beta-stimulated expression of iNOS in human osteoarthritis chondrocytes.
- Inhibition of INOS protects T-lymphocytes from hypoxia-induced apoptosis.
- An increase in reactive oxygen species activity upregulates eNOS/iNOS expression in cultured endothelial cells, and in vivo in animals.
- The expression of iNOS induces oxidative stress in nasopharyngeal carcinoma. p53 mutation was associated with iNOS overexpression. This suggests that the p53 gene is not the direct target of DNA damage by 8-OHdG accumulation.
- INOS gene silencing prevents infl;ammatory cytojkinde-induced beta-cell apoptosis.
- Increased expression of iNOS might have a role in the development of allergic inflammation in upper and lower airways and in comorbidity of allergic rhinitis and asthma.
- A previously unknown role for HMGA1 in the regulation of hNOS2, is demonstrated.
- Hypoxia regulates iNOS expression in human normal peritoneal and adhesion fibroblasts through nuclear factor kappa B activation mechanism.
- Carbon monoxide exposure decreases NO production by suppressing dimer formation and increasing iNOS degradation through a process involving p38 activation
- activity of the iNOS protein containing the A805D mutation was comparable to wt, while a drastic reduction in iNOS activity was observed for the F831S and L832P mutants
- Expression of this isoform is reduced in skeletal muscle in type 1 diabetes.
- expression of iNOS in patient tumors was found to associate with nitrotyrosine, COX2, pSTAT3, and arginase
- TLR-7 signaling stimulates apoptosis resistance, associated with enhanced iNOS expression (protein and mRNA) and NO release, notably through an NF-kappaB-dependent activation of the NO pathway.
- within these samples iNOS expression is increased and associated with increased NO metabolite production
- genetic polymorphisms of HO-1 and iNOS modulate individual susceptibility to gastric cancer risk.
- Down-regulation of HO-1 expression in neutrophils from hypertensive subjects may be exerted through the inhibition of NOS2 expression.
- iNOS plays roles in promoting pancreatic carcinogenesis in both early and late stages in hamsters and in humans.
- findings indicate that iNOS cross talk with NF-kB and Ha-RAS/ERK cascades influences hepatocellular carcinoma growth and prognosis
- 50 polymorphisms within NOS2A gene or flanking the locus showed no evidence for any individual SNP allele or haplotype in Multiple sclerosis-affected individuals was observed.
- The expression of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and inducible nitric oxide synthase (iNOS) in a verrucous proliferative leukoplakia sample, was investigated.
- In innate immune responses to Acinetobacter baumannii OmpA, the surface expression of Toll-like receptor 2 and the production of inducible nitric oxide synthase (iNOS) were prominently observed in HEp-2 cells.
- The interaction between smoking and genetic variants of eNOS and iNOS predicts survival after stroke, especially among postmenopausal women.
- the constitutively activated melanoma MAPK pathway stimulates activation of NF-kappaB hetero- and homodimers, which, in turn, drive iNOS expression and support melanoma tumorigenesis
- nNOS was more frequently expressed in normal than in osteoarthritic chondrocytes, whereas the opposite was found for iNOS; IL-1 induced the degradation of both enzymes, but iNOS disappeared more rapidly
- analysis of the the (CCTTT)n polymorphism in patients with asthma and nasal polyposis; the number of CCTTT repeats in the promoter region of NOS2A could be associated with the inflammatory process of nasal polyposis in this population
- analysis of signal-mediated functional activation of inducible nitric-oxide synthase and its role in stimulating platelet activation
- anti-NOS2A RNA is involved in the regulation of neuronal differentiation of hESCs through the modulation of NOS2A gene expression.
- significantly elevated in placenta in fetal growth restriction
- YC-1 stimulates the expression of INOS in vascular smooth muscle cells.
- IFN-gamma exerted a delayed suppressive effect on K(+) channel activity by enhancing iNOS expression and an acute stimulatory effect, which was independent of either NO pathways or phosphorylation processes mediated by PKA, PKG, and PI3K in RPTECs.
- The amyloid-beta protein precursor NOS2(-/-) bigenic mice progress from production and amyloid deposition to hyperphosphorylated normal mouse tau at Alzheimer's disease associated epitopes--REVIEW
- These data suggest an important role for nitric oxide in ovarian carcinogenesis, and show that inducible nitric oxide synthase expression in well-differentiated carcinomas is greater than in moderately/poorly differentiated carcinomas.
- These results suggest that bronchial epithelial cells used the physiologic aggresome mechanism for iNOS inactivation.
- LDL and UV-oxidized LDL significantly enhance the expression of iNOS and NO production in unstimulated macrophages and similarly in HUVEC.