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Validated All-in-One™ qPCR Primer for NGF(NM_002506.2) Search again
Product ID:
HQP011827
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
Beta-NGF, HSAN5, NGFB
Gene Description:
nerve growth factor
Target Gene Accession:
NM_002506.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons.
Gene References into function
- On immunologic stimuli, human eosinophils secrete nerve growth factor that promotes elongation of neurites.
- Results support the involvement of NGF, BDNF, leptin, and mast cells in human coronary atherosclerosis and metabolic syndrome, implying neuroimmune and adipoimmune pathways in the pathobiology of these cardiovascular disorders.
- Phenotypic knockout of nerve growth factor (NGF) activity in transgenic anti-NGF mice (AD11 mice) results in a progressive neurodegenerative phenotype.
- The gene expression of this protein was studied in the developing human tooth.
- correlation between the activity of systemic lupus erythematosus and the levels of NGF in serum
- NGF modulates cytokine mRNA expression in immune cells. (NGF)
- Interleukin-10 and nerve growth factor have reciprocal upregulatory effects on intestinal epithelial cells.
- Data suggest that activation of bronchial eosinophils by neurotrophins (nerve growth factor, brain-derived neurotrophic factor, neurotrophins-3 and -4) might play a role in the regulation of eosinophilic inflammation in allergic asthma.
- Circulating levels in Behcet disease are not different from normal values.
- histamine may enhance nerve growth factor production by inducing c-Fos expression in keratinocytes.
- NGF might have a crucial role in the auditory pathway, promoting the survival and preventing the degeneration of sensorineural cells.
- NGF is expressed in human pancreatic beta cells and modulates insulin secretion through a paracrine/autocrine loop, similar to the one observed in cultured rat beta cells
- Zinc(II) in native beta-NGF plays an important role in the structure and the biological activity of the protein
- mutation causes loss of pain perception
- acts as a co-receptor and molecular switch governing the p75NTR-mediated pro-apoptotic signal induced by proNGF
- determined the 2.4 A crystal structure of NGF complexed with the extracellular domain of p75; the complex is composed of an NGF homodimer asymmetrically bound to a single p75
- expression of NGF and its receptors, TrkA and p75NTR, in hepatocellular carcinomas (HCC); NGF and its receptors are thought to have a role in cellular interactions involving HCC cells, hepatic stellate cells, arterial cells and nerve cells in HCC tissues
- Monocytes, produce, store and release NGF, brain-derived neurotrophic factor and neurotrophin 3
- pro-NGF purified from AD human brains can induce apoptosis in neuronal cell cultures through its interaction with the p75NTR receptor
- NGF is indeed involved in growth initiation of human primordial follicles.
- p62 regulates nerve growth factor-induced NF-kappaB activation by influencing TRAF6 polyubiquitination
- NGF rapidly increases membrane expression of TRPV1 heat-gated ion channels
- Overexpression in salivary adenoid cystic carcinoma may constitute a reason for perineural invasion
- Light and electron microscopy immunohistochemistry showed that tonsillar samples were positive for NGF.
- Here, we show the first evidence of NGF-TrkA autocrine loop and clinical significance of Nerve growth factor(NGF)overexpression in oesophageal squamous cell carcinoma(OESCC).
- non-fat cells in human adipose tissue contribute to most of the release of NGF seen during primary culture of adipose tissue explants from obese women
- Findings report, for the first time, the expression pattern of NGF and TrkA proteins in human scalp skin and hair follicle.
- SNP associated with ADHD in the 5' pro-NGF sequence may affect intracellular processing and secretion of NGF.
- a NGF/BEX2/NF-kappaB pathway is involved in regulating apoptosis in breast cancer cells and in modulating response to tamoxifen in primary tumors
- We show here high expression of TRKA in Hodgkin-Reed/Sternberg cell lines as compared to normal B cells and other B cell lymphomas, without major increases in TRKA gene dosage.
- has mitogenic and motogenic effects on oral mucosal keratinocytes. Differential expression throughout the epithelium suggests a role in epithelial differentiation
- Nerve growth factor was only detected in liver tissue with hepatocellular carcinoma present.
- NGF concentration in CSF is a useful marker of brain damage following severe TBI
- Late preweaning exposure of rats to chlorpyrifos and methyl parathion affect expression of nerve growth factor in hippocampus and cerebral cortex.
- The NGF mRNA levels at baseline in the interstitial cystitis patients were significantly greater than those in the controls. NGF expression plays an important role in the pathogenesis of IC.
- These results newly demonstrate the cardiac prosurvival action of NGF and provide mechanistic information on the signaling pathway
- These data support a role for NGF-induced cell apoptosis in LLS formation in vitro.
- N-terminal Vps10p domain of sortilin, which is responsible for the interaction with the neurotrophins, adopts a beta-propeller fold, and that the N-terminal regions of sortilin, pro-NGF and pro-BDNF are mainly intrinsically disordered regions
- Targeting NGF in breast cancer may have therapeutic ramifications.
- Variants in NGFB gene appear to modify the risk conferred by the NTRK3 rs7180942 risk genotypes showing a synergistic epistatic interaction.
- can play a role in the pathology of oral lichen planus
- Analyzed gene expression profile of NGF-treated prostate cancer cell lines.
- Data show that nerve growth factor levels in corneal epithelial cells were up-regulated during hyperosmolar stress by IL-1beta, and suggest that enhanced NGF expression may be aid in recovery from corneal damage due to chronic hyperosmolar stress.
- NT-3, NT-4, and nerve growth factor (NGF) induce cell migration in melanoma, with a stronger effect on metastatic cell lines.
- keratinocytes in patients with psoriasis are primed to produce elevated levels of NGF
- A family with neurological findings similar to hereditary sensory and autonomic neuropathy type V having a point mutation in the nerve growth factor beta gene.
- Amyloid precursor protein-overexpressing transgenic mice show a genotype-dependent increase of cortical NGF that is highly correlated with amyloid concentrations, which may reflect an amyloid-related glia-derived secretion or altered axonal transport.
- The present study pinpoints loop II of NGF to be an important structural determinant for NGF biological activity mediated by TrkA receptor.
- Both the annulus fibrosus and nucleus pulposus cells of the intervertebral disc (IVD) express the neurotrophins NGF and BDNF, factors that may influence and enhance innervation and pain in the degenerate IVD.
- anxiety relates to a genetic variation of NGF and that genes modulate behaviour in a gender-dependent manner.
- We conclude that in CSF of AD patients (at least partly) mature NGF-like immunoreactivity is enhanced, and is masked in a bioassay by the toxic properties of CSF
- Nerve-growth factor (NGF) and brain-derived neurotrophic factor (BDNF) levels are potential biomarkers for lymphocyte activation in primary Sjogren's syndrome patients.
- Possible role of nerve growth factor and interleukin-18 in pathogenesis of eczematous lesions of atopic dermatitis.
- The level of horn nerve growth factor was found to reflect the severity of itching and eruptions in atopic dermatitis.
- study describes the effects of the homozygous missense (R221W) mutation on the function of NGF protein and the molecular mechanisms that may underlie its pain insensitivity phenotype
- decreased plasma levels in chronic urticaria patients resistant to H1 antihistamines; in treatment-responsive group, suppression of symptoms is accompanied by an increase in serum concentration af
- NGFB variation and expression levels appear as modulating factors in the development of multiple sclerosis
- preferential NGF localization in mucous cells of the epithelial lining and in the submucosal glands suggests a possible role for NGF in modulating secretion in allergic rhinitis and possibly other allergic diseases.
- For the 1st time, an increased NGF concentration was shown in glomerulonephritis & hemodialysis patients compared to controls. NGF can act as protective factor against cytotoxic injuries.
- Nerve growth factor (NGF) concentration in the CSF is increased following traumatic head injury in children and thus ia a useful biological marker of brain damage.