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Validated All-in-One™ qPCR Primer for NEUROD1(NM_002500.4) Search again
Product ID:
HQP011771
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
BETA2, BHF-1, MODY6, NEUROD, T2D, bHLHa3
Gene Description:
neuronal differentiation 1
Target Gene Accession:
NM_002500.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box.
Gene References into function
- regulator of insulin transcription
- expression during trophoblast invasion
- Beta-cell dysfunction in late-onset diabetic subjects carrying homozygous mutation in transcription factor NeuroD1.
- The genetic polymorphism in NeuroD is associated with the development of early-onset type 2 diabetes. The presence of Thr45 allele may represent a risk factor for early-onset type 2 diabetes among Chinese.
- Polymorphism Ala45Thr is associated with Type 1 diabetes mellitus in Czech children
- Ala5Thr polymorphism of NeuroD1 plays a role in the risk of NIDDM in the examined Polish population
- NeuroD1/E47 transcription factors up-regulate IA-1 gene expression through the proximal E-box element of the IA-1 promoter
- Ala45 variant of BETA2/NeuroD1 may be associated with IDDM in Caucasians.
- NeuroD1 is differentially expressed in pituitary adenomas and its possible ontogenetic and/or pathogenetic implications in non-corticotroph tumors are discussed.
- No evidence of Ala(45)Thr polymorphism of the NeueroD gene and type 1 diabetes.
- Focus of this review is on recent progress in understanding the important role of BETA2/NeuroD1 in initiating neuronal differentiation and maintaining the nervous system.
- Polymorphism contributes to glucose intolerance in a South Indian population.
- NeuroD controls both common and distinct sets of molecules involved in cell survival and differentiation in different tissue types [review]
- co-expression and functional synergism of these beta-cell enriched transactivators, MafA, Pdx1, and Beta2, are critical for establishing the beta-cell-specific and efficient expression of the insulin gene.
- the SREBP-1c.BETA2.E47 complex is in a DNA looping structure which is required for efficient recruitment of CREB-binding protein/p300
- we demonstrated that ISL1 and BETA2 could activate insulin gene transcription synergistically.
- Gender-specific association of the Ala45Thr variant of NEUROD1 with Type 1 diabetes in Brazilian women.
- results presented in this study define INSM1 as a transcriptional repressor of the neuroD/b2 gene. The molecular mechanism of INSM1 transcriptional repression is attributed to the recruitment of cyclin D1 and HDAC-1 and -3
- The NeuroD1-Ala45Thr variation may itself have an important role in susceptibility to or be in disequilibrium with early-onset T2DM in Chinese. The Ala45Thr may affect the onset pattern of T2DM, i.e., early-onset but not late-onset T2DM in Chinese.
- helix-loop-helix (HLH) domain of basic helix-loop-helix (bHLH) family proteins such as NeuroD facilitate protein transduction into various cell lines.
- Expression of NeuroD1 versus chromogranin-A is more frequent in pCA, and correlates to increased indicators of malignancy in moderately to poorly differentiated pCA.
- These results suggest that NeuroD plays an important role in regulated exocytosis by inducing expressions of various components required in the process.
- A study evaluating the extent to which common variation in the six known maturity-onset diabetes of the young (MODY) genes, which cause a monogenic form of type 2 diabetes, is associated with type 2 diabetes is presented.
- Mutation in the NeuroD1/BETA2 gene contributes to the development of diabetes
- Expression of NeuroD1 versus chromogranin-A is more frequent in pCA, and correlates to increased indicators of malignancy in moderately to poorly differentiated pCA.
- We report a novel disease-associated variant in NEUROD1 identified among a set of MODYX families
- NeuroD has a role in the regulation of pulmonary neuroendocrine and alveolar morphogenesis
- NEUROD1 methylation is a chemosensitivity marker in estrogen receptor-negative breast cancer.
- High NeuroD expression was seen in all well-differentiated gastroenteropancreatic neuroendocrine carcinoma and tumor (carcinoid) patients.