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Validated All-in-One™ qPCR Primer for NEK2(NM_002497.3) Search again
Product ID:
HQP011764
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
HsPK21, NEK2A, NLK1, PPP1R111, RP67
Gene Description:
NIMA related kinase 2
Target Gene Accession:
NM_002497.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- Nek2.PP1C complex is regulated by Inh2 via inhibition of phosphatase activity to initiate centrosome separation
- cell cycle-regulated serine phosphorylation of Hec1 by Nek2 is essential for faithful chromosome segregation
- Nek2A function is required for the correct execution of mitosis
- NEK2A is a kinetochore-associated protein kinase essential for faithful chromosome segregation
- Nucleolar Nek11 is a novel target of Nek2A in G1/S-arrested cells
- Nek2 is involved in activation of the G2 checkpoint and is not secondary to cell cycle arrest
- Nek2 protein is significantly up-regulated in preinvasive in situ ductal carcinomas of the breast as well as in invasive breast carcinomas.
- multiple processes involved in regulating the abundance of Nek2 kinase at the centrosome including microtubule binding, the centriolar satellite component pericentriolar material 1, and localized protein degradation
- Down-regulation of NEK2A does not lead to mitotic defects; absence of the isozyme NK2B, a splice variant, leads to a mitotic delay and the formation of multinucleated cells.
- summary of the expression, regulation and function of Nek2 and its roles in human cancer [review]
- DNA damage-induced inhibition of Plk1 leads to inhibition of Nek2 activity and thus prevents centrosome separation
- Mutational analysis of autophosphorylation sites identified by mass spectrometry and x-ray structure show a complex pattern of positive and negative regulatory effects on kinase activity correlated with effects on centrosomal splitting efficiency
- Radiation-induced inhibition of centrosome splitting was abrogated in cells expressing Nek2 mutated in the PP1-binding motif outside the kinase domain.
- Results report that NIP2, previously identified as centrobin, is a novel substrate of Nek2, and that NIP2 has a role in stabilizing the microtubule structure.
- Results suggest that NEK2A-mediated phosphorylation of Sgo1 (SGOL1) provides a link between centromeric cohesion and spindle microtubule attachment at the kinetochores.
- alternative splicing provides an unusual mechanism for modulating Nek2 localization, enabling it to have both nuclear and cytoplasmic functions.
- Nek2 binds and phosphorylates beta-catenin.
- that Hec1 binds to microtubule in low affinity and phosphorylation by NEK2A, which prevents aberrant kinetochore-microtubule connections in vivo, increases the affinity of the Ndc80 complex for microtubules in vitro
- Nek2 plays a critical role in carcinogenesis, tumor invasion, and tumorigenic growth of breast carcinoma.