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Validated All-in-One™ qPCR Primer for NCL(NM_005381.2) Search again
Product ID:
HQP011698
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
C23, Nsr1
Gene Description:
nucleolin
Target Gene Accession:
NM_005381.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq].
Gene References into function
- Anchorage of HIV particles on permissive cells induces aggegation of surface nucleolin and its association with detergent-insoluble lipid raft components. Moreover, surface nucleolin and lipid rafts are implicated in the HIV entry process.
- functions in the biogenesis and/or trafficking of hY1 and hY3 RNPs through the nucleolus and subsequent transport to the cytoplasm
- These data indicate a novel p53-dependent mechanism in which cell stress mobilizes nucleolin for transient replication inhibition and DNA repair.
- results suggest that the cell surface-expressed nucleolin serves as a low affinity receptor for midkine and could be implicated in its entry process
- change in levels and localization accompanies apoptosis in leukemia cells
- Data suggest that changes in nucleolin or argyrophilic nucleolar organizer region proteins occur in cells induced to undergo apoptosis by okadaic acid and by other stimuli.
- Results show that nucleolin interacts with centaurin-alpha(1) protein, suggesting that centaurin-alpha(1) may be part of a ribonucleoprotein complex.
- Bidirectional DNA unwinding is achieved by a ternary complex of T antigen, this protein and DNA topoisomerase I.
- we propose a autoregulatory model in which Nucleolin regulates the translation of Nucleolin mRNA, such that during a period of excess protein, translation is inhibited through direct binding of Nucleolin protein to its mRNA.
- The cell surface molecule recognized by F3 is nucleolin. Nucleolin specifically bound to an F3 peptide affinity matrix from extracts of cultured breast carcinoma cells.
- nucleolin functions as a bcl-2-stabilizing factor and that taxol and okadaic acid treatment induces apoptosis in HL-60 cells through a process that involves down-regulation of nucleolin and destabilization of bcl-2 mRNA
- interaction of hTERT and nucleolin participates in the dynamic intracellular localization of telomerase complex
- Retinoic acid-induced apoptosis is a consequence of cellular differentiation, which leads to nucleolin down-regulation and bcl-2 mRNA instability.
- identification of N317 and N492 as the two N-glycosylation sites
- Nucleolin was detected at the surface of macrophages, and anti-nucleolin antibody inhibited the binding of the early apoptotic cells to macrophages.
- RNA/protein interaction studies show binding of nucleolin to 3'UTR may be of major importance for increased efficiency of MMP-9 translation.MMP-9 expression can be regulated posttranscriptionally, affecting efficiency of translation & localization of mRN
- results demonstrate that PTN inhibits HIV infection and suggest that the cell surface-expressed nucleolin is a low affinity receptor for PTN binding to cells and it is also implicated in PTN entry into cells by an active process
- nucleolin and poly[ADP-ribosyl] transferase have roles in drug resistance
- Changes in the subcellular distribution and expression level of nucleolin play an important role in intestinal cell differentiation and relay the signaling pathway induced by laminin-1.
- The present results indicate that PP1 and nucleolin were differently expressed at G(1)/S and G(2)/M boundaries of the cell cycle and acted in a different fashion during cell-cycle progression.
- VEGF, extracellular matrix, and intracellular motor protein MyH9 are all essential for the novel function of nucleolin in angiogenesis.
- 3'UTR-bound nucleolin enhances mRNA stability of beta-globin
- nucleolin binds the KLF2 promoter
- In cells transfected with both IRF-2 and p300/CBP-associated factor , IRF-2 associated with endogenous nucleolin.
- Nucleolin greatly enhances the activity of the chromatin remodeling machineries SWI/SNF and ACF
- intracellular distribution of nucleolin in epithelial cells is Rb-dependent, and an altered nucleolin localization in human cancerous tissues results from a loss of Rb
- nucleolin, like ARF, responds to hyperproliferative signals by upregulation of p53 through Hdm2 inhibition
- cross-dependence between aprataxin and nucleolin in the nucleolus
- data provide evidence of a nucleolar localization of K-Ras4B and describe a functional association between K-Ras4B and nucleolin
- The functional consequences of the identified PIDD/nucleolin interaction remain to be elucidated, but may be related to a recently discovered new role for PIDD in the activation of NF-kappaB upon genotoxic stress.
- Nucleolin was identified as a protein that allows RNA polymerase II to transcribe nucleosomal templates in vitro.
- nucleolin acts as a component of the gene regulation program of CD34-positive hematopoietic
- Results indicate that nucleolin is required for nucleolus formation, and is also involved in chromosome congression and spindle formation.
- Nucleolin interacts with the AP-1 site within the promoter sequence of the metalloproteinase-13 gene.
- The repressive activity of GZF1 is modulated by the level at which nucleolin is expressed.
- Data show that, in addition to the expected effects on pre-ribosomal RNA accumulation and nucleolar structure, the absence of nucleolin results in a cell growth arrest, accumulation in G2, and an increase of apoptosis.
- a novel function of Influenza A virus NS1, namely, affecting cellular events via interaction with nucleolin
- Evidence that BIG1 and nucleolin, but not fibrillarin, can be present with p62 at the nuclear envelope confirms the presence of BIG1 and nucleolin in dynamic molecular complexes that change in composition while moving through nuclei
- the nucleolus exhibits an osmotically regulated gatekeeping activity that controls the spatial dynamics and functions of nucleolin
- Using small interfering RNA technology, we show that viral replication requires a high level of nucleolin expression, demonstrating for the first time a direct role for a nucleolar protein in herpes simplex virus biology.
- Results show that nucleolin is a signaling receptor for P-selectin on Colo-320 cells and suggest a mechanism for linkage of nucleolin to signal transduction pathways that regulate adhesion and spreading of Colo-320 on fibronectin substrates.
- Although S100A11 is initially expressed in the cytoplasm, it is transported to the nucleus by the action of nucleolin.
- Low nucleolin expression was the single most important biological predictor of outcome in pediatric intracranial ependymoma.
- the interaction between surface nucleolin and its bacterial ligand EF-Tu plays an important role in Francisella tularensis adhesion and entry process and may therefore facilitate invasion of host tissues
- Binding of an extracellular ligand to surface nucleolin could be involved in the activation of signaling pathways by promoting Ca(2+) entry into cells.