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Validated All-in-One™ qPCR Primer for MTAP(NM_002451.3) Search again
Powered by BiozBy default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. [provided by RefSeq].
Gene References into function
- Methylthioadenosine phosphorylase, a gene frequently codeleted with p16(cdkN2a/ARF), acts as a tumor suppressor in a breast cancer cell line.
- Results suggest an important role of methylthioadenosine phosphorylase inactivation in the development of melanomas.
- Methylthioadenosine phosphorylase regulates ornithine decarboxylase by production of downstream metabolites
- MTAP is not expressed in normal human colonic epithelium but is strongly upregulated in colon carcinoma.
- epigenetic mechanisms, involving DNA methylation and histone deacetylation, may play a role in the silencing of MTAP gene expression in hepatocarcinoma
- MTAP activity is frequently lost, and ODC activity is frequently elevated in both pancreatic adenocarcinoma and neuroendocrine tumors
- MTAP expression is lost in approximately 30% of infiltrating pancreatic cancers and in a lower percentage of other periampullary neoplasms; loss is a result of homozygous deletions encompassing both the MTAP and p16INK4A/CDKN2A genes
- Results demonstrated concordant loss of MTAP and p16 protein expression in pancreatic intraepithelial neoplasia.
- MTAP inactivation is associated with hepatocellular carcinoma development and invasiveness
- Selective loss at the 3' end of MTAP was observed in head and neck squamous cell carcinoma cell line UMSCC-11A.
- The frequency of Methylthioadenosine phosphorylase (MTAP) deletions in conventional, grade II chondrosarcomas by fluorescence in situ hybridization (FISH) analysis was investigated.
- Methylthioadenosine phosphorylase (MTAP) gene deletion and lack of protein expression are associated with poor prognosis in mantle cell lymphoma.
- Regulation of MTAP by reactive oxygen species might participate in the redox regulation of the methionine catabolic pathway in the liver