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Validated All-in-One™ qPCR Primer for MT3(NM_005954.3) Search again
Product ID:
HQP011539
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
GIF, GIFB, GRIF, ZnMT3
Gene Description:
metallothionein 3
Target Gene Accession:
NM_005954.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- MT-3 expression is involved in the transport function of a human renal cell line that retains properties of the proximal tubule.
- Overexpression of human metallothionein-III prevents hydrogen peroxide-induced oxidative stress in human fibroblasts. "metallothionein-III "
- overexpression can influence the growth and chemotherapeutic drug resistance of the PC-3 prostate cancer cell line
- Hypermethylation of metallothionein-3 CpG island in gastric carcinoma
- Metallothionein-III has anticarcinogenic and neuroprotective roles in cells exposed to gamma rays
- The affect the role of MT3 on cell viability, which may explain in part why the comprehensive effect of MT3 on the cells was elusive.
- MT3 expression is frequently down-regulated in oesophageal squamous cell carcinoma , by DNA methylation, but that this is not a prognostic indicator
- mutation of MT3 at Glu23 may alter the NO metabolism and/or affect zinc homeostasis in brain, thus altering the neuronal growth inhibitory activity
- The alpha-domain is indispensable and plays an important role in modulating the stability of the metal cluster in the beta-domain by domain-domain interactions, thus influencing the bioactivity of hMT3.
- The level of MT-3 expression in human proximal tubular cells influences transepithelial resistance and cadherin expression but does not the Cd(+2)-induced loss of vectorial active transport.
- MT-3 is a highly hypoxia-inducible gene in human adipocytes; the protein may protect adipocytes from hypoxic damage
- The goals of this study were to define the expression of the isoforms of MT 1, 2, 3 at both mRNA and protein levels, in normal prostate, benign prostatic hyperplasia (BPH) and malignant PC-3 cells.
- These results suggest that MT-III upregulates VEGF production in brain endothelial cells by a HIF-1alpha-dependent mechanism.
- The structure adopted by the (6)CPCP(9) motif is the determinant factor of the inhibitory bioactivity of hGIF; but residues within the N-terminal fragment may also influence the peptide conformation and contribute to the protein's bioactivity.
- Metallothionein-III-induced activation of phosphatidylinositol 3-kinase/Akt and extracellular signal-regulated kinase1/2 up-regulates expression and activity of heme oxygenase-1, which provides protection against oxidative damage in dopaminergic cells.
- the bioactivity of hGIF is mainly related to the essential metal release and its characteristic conformation.
- metallothionein in decidual cells seems to be responsible for the proper coexistence between decidual cells and activated immune cells that infiltrate both eutopic and ectopic decidua during cesarean section and placental abruption