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Validated All-in-One™ qPCR Primer for MSN(NM_002444.2) Search again
Product ID:
HQP011519
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
HEL70, IMD50
Gene Description:
moesin
Target Gene Accession:
NM_002444.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement.
Gene References into function
- amino-terminal domains of the ezrin, radixin, and moesin (ERM) proteins bind advanced glycation end products and have a role in diabetes
- PECAM-1 co-localized with moesin at the cell periphery and in filopodia of glass-activated platelets; moesin may play a role in platelet adhesion, linking PECAM-1 with the actin cytoskeleton
- moesin has a role in lymph node metastasis of oral squamous cell carcinoma
- important role in lipopolysaccharide-induced TNF-alpha production, particularly in lipopolysaccharide-mediated signal transduction
- ezrin/radixin/moesin proteins are recruited by NHE1 Na+/H+ exchanger and have roles in regulating Akt-dependent cell survival
- All three ERM family members can localise to the nucleus; a specific nuclear localisation sequence, which is conserved and functional in all ERM family members, is identified, implying specific regulated nuclear import.
- there is nucleotide variation at Msn and Alas2 on the X chromosome
- results suggest that ezrin-radixin-moesin proteins are required for microvillar positioning of L-selectin and that this is important both for leukocyte tethering and L-selectin shedding
- Ezrin, radixin and moesin are ADP-ribosylated by Pseudomonas aeruginosa ExoS
- Expression of ExoS in HeLa cells led to a loss of phosphorylation of Ezrin/radixin/moesin proteins that was dependent upon the expression of ADP-ribosyltransferase activity.
- early phagosome maturation was shown to depend on activation of Rho acting through Rho kinase on ezrin-radixin-moesin proteins
- B-cell response to moesin, possibly derived from hematopoietic cells, frequently occurs in patients with acquired aplastic anemia
- moesin negatively regulates stable microtubule networks and is a natural determinant of cellular sensitivity to retroviral infection
- increased CD44, ezrin, radixin, and moesin phosphorylation represents a key molecular abnormality that guides T cell adhesion and migration in SLE patients.
- MSN was differentially expressed in sclerotic hippocampi compared to non-sclerotic ones.
- Moesin protein expression is a basal marker in breast tumors. Moesin expression was nearly an independent prognostic marker of poor outcome
- the ezrin, radixin, and moesin proteins function as positive regulators of infection by X4-tropic HIV-1
- Phosphorylation of ezrin/moesin by ROCK is involved in the early steps of apoptotic signaling following Fas receptor triggering and regulates apoptosis induction in Jurkat cells.
- functional alterations of moesin may be involved in pathological disorders associated with clathrin-mediated internalization or receptor recycling
- Results suggest that activated moesin promotes F-actin redistribution and CD4-CXCR4 clustering and is also required for efficient X4-tropic HIV-1 infection in permissive lymphocytes.
- No aspects of T cell receptor signaling uniquely require transgenic ezrin or moesin; instead, T cell activation appears to depend on net ezrin, radixin, and moesin (ERM) protein expression, while ezrin and moesin function together.
- c14orf166 was identified asa novel metastasis-associated protein, and the roles of radixin, moesin and c14orf166 in pancreatic cancer metastasis deserve further investigations.