|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for CD99(NM_002414.4) Search again
Product ID:
HQP011212
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
HBA71, MIC2, MIC2X, MIC2Y, MSK5X
Gene Description:
CD99 molecule (Xg blood group)
Target Gene Accession:
NM_002414.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Summary
The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene.
Gene References into function
- Results suggest that type I is the major isoform of CD99 expressed in non-neoplastic gastric mucosa and gastric adenocarcinomas.
- Functional involvement of src and focal adhesion kinase in a CD99 splice variant-induced motility of human breast cancer cells
- engagement triggers the exocytic transport of ganglioside GM1 and the reorganization of actin cytoskeleton
- inhibition by MHC class I engagement or apoptosis and upregulation of T cell receptor and MHC molecules in human thymocytes and T cell line induced by CD99
- CD99 epitopes plays a distinct role in T cell biology, especially in T cell apoptosis.
- CD99 type II acts as a negative regulator in the neural differentiation of precursor cells that might occur during nerve system development
- Solitary sclerotic fibroma is a fibrotic lesion with cells positive for CD34 and O13. O13 expression in SF has not been previously described.
- our findings indicate that CD99 functions occur through reorganization of cytoskeleton and identify actin and zyxin as the early signaling events driven by CD99 engagement.
- solution structure of cytoplasmic domain of human CD99 type I shows that it has a hairpin shape anchored by two flexible loops
- cyclophilin A binds CD99 and may be either a signaling mediator or a signaling regulator for CD99
- loss of CD99 expression in PETs was found to be associated with ominous prognostic indicators
- Cooperation of CD99 engagement with suboptimal TCR/CD3 signals resulted in enhanced CD4+ T cell proliferation, elevated expression of CD25 and GM1, increased apoptosis, augmented activation of JNK, and increased AP-1 activation
- CD99 was found to be widely expressed in both sarcomatous and carcinoma component in pleomorphic carcinomas of the lung
- High expression of CD99 in ALK-positive anaplastic large cell lymphomas is an unexpected finding and its biologic and clinical significance has yet to be clarified.
- The findings point to an antioncogenic role for CD99 in osteosarcoma, likely through the regulation of caveolin-1 and inhibition of c-Src kinase activity.
- LMP-1 induced down-regulation of the CD99 pathway is important in nasopharyngeal carcinogenesis, and the expression of CD99 in lymphoid stroma may regulate immune response to nasopharyngeal carcinoma.
- LMP-1 induced down-regulation of the CD99 pathway is important in nasopharyngeal carcinogenesis, and the expression of CD99 in lymphoid stroma may regulate immune response to nasopharyngeal carcinoma. (LMP-1= EBV latent membrane protein 1)
- subsets of CD34+ cells with high or low levels of CD99 expression produce different numbers of erythroid, natural killer (NK), or dendritic cells in the in vitro differentiation assays
- The results of this study suggest that expression of a splice variant of CD99 contributes to the invasive ability of human breast cancer cells by up-regulating AP-1-mediated gene expression through the Akt-dependent ERK and JNK signaling pathways.
- PECAM (CD31) and CD99 regulate distinct, sequential steps in the transendothelial migration of neutrophils during inflammation
- These data demonstrate the unique properties of CD99 co-stimulation that distinguish this molecule from CD28 and other raft-resident co-stimulatory factors.
- Our findings highlight the involvement of CD99 in crucial processes of cancer malignancy.
- CK19/CD99 immunoexpression did not correlate with extent of tumor invasion, mitotic activity, Ki-67 labeling index, presence of extracellular mucinous pools dissecting muscle, and angiolymphatic and perineural/neural invasion in goblet cell carcinoids.
- CD99-immunohistochemistry is unique in that it is helpful for the diagnosis of clear cell brain tumors through the visualization of CD99-negative clear cells
- CD99 also binds p230/golgin-245, a coiled-coil protein that recycles between the cytosol and buds/vesicles of the TGN and which plays a fundamental role in trafficking transport vesicles.