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Validated All-in-One™ qPCR Primer for CD46(NM_172351.2) Search again
Product ID:
HQP011116
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
AHUS2, MCP, MIC10, TLX, TRA2.10
Gene Description:
CD46 molecule
Target Gene Accession:
NM_172351.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. This gene is found in a cluster on chromosome 1q32 with other genes encoding structural components of the complement system. At least fourteen different transcript variants encoding fourteen different isoforms have been found for this gene.
Gene References into function
- CD46 costimulation induces morphological changes in T cells and activates the MAP Kinase Signaling System
- peripheral blood monocytes treated for 5--8 days with GM-CSF (i.e. mature Mphi) acquired the capacity to assemble CD9, alpha3-beta1 integrin and the tyrosine phosphatase SHP-1 with their CD46
- The findings reported here strongly suggest that pilus binding to CD46 is not a simple static process. Rather, they support a model in which pilus interaction with CD46 promotes signaling cascades important for Neisseria infectivity.
- Production and characterization of a pig line transgenic for human membrane cofactor protein.
- required for HHV-6A induced fusion from without
- For the classical pathway, MCP is the cofactor for C4b cleavage (and factor H for C3b cleavage); however, if the alternative pathway mediates C3b deposition, then MCP's cofactor activity is sufficient to restrict complement activation.
- CD46 plays a role in regulating T cell-induced inflammatory reaction and in fine-tuning the cellular immune response by bridging innate and acquired immunity.
- CD46 isoforms function as receptors for human herpesvirus 6 and measles virus
- Co-engagement of CD3 and the complement regulator CD46 in the presence of IL-2 induces a Tr1-specific cytokine phenotype in human CD4+ T cells
- Human herpesvirus 6 variant A glycoprotein H-glycoprotein L-glycoprotein Q complex is a viral ligand for human CD46
- CD46 has a role as a receptor for fusion and entry of human herpesvirus 6 into target cells
- role in meningococcal disease; results suggest that CD46 facilitates pilus-dependent interactions at the epithelial mucosa
- Adenovirus type 11 uses CD46 as a cellular receptor.
- elucidation of two pathways for CD46 internalization, which are regulated by the valence of cross-linking of CD46 and which utilize either clathrin-coated pits or pseudopodial extension
- Mutations predispose to development of familial hemolytic uremic syndrome.
- Data show that CD46 affords protection from antibody-mediated rejection when mouse anti-pig serum was administered to scid mice bearing grafts from either CD46 transgenic or normal pigs.
- CD46 is localized to the plasma membrane and may have a role in the development of breast cancer epithelial cells
- Highly efficient CD46 function, controlling both classical and alternative pathway complement activation, which suggests it might be the complement regulator of choice to protect kidney xenografts from pig to papio.
- In pigs transgenic for human MCP, the transgene is expressed at similar levels to that in human embryonic neural tissue but at an insufficient level to prevent activation of the complement cascade.
- serves as an attachment receptor for adenovirus-37
- all of the major splice forms of CD46 are predominant and functional binding sites of species B Adenovirus 3 on CD46-expressing rodent and human cells but may not be the sole receptor of species B Ads on human cells
- Review discussing role of CD46 mutations in predisposition to hemolytic uremic syndrome.
- Significant difference in intensity of the staining of CD55 and CD46 among cells in various layers of normal esophageal mucosa and esophageal carcinoma cells, but not in the staining of CD59.
- CD46 costimulation promotes a Th1-biased response in human CD4+ T lymphocytes. CD46 enhanced TCR/CD3-induced tyrosine phosphorylation of CD3zeta and ZAP-70.
- functional activity of CD46 is not restricted to the tumor cell membranes but can be liberated in vesicles and by a metalloproteinase.
- binding site with measles virus hemagglutinin: attachment sites for MV receptors SLAM and CD46 overlap on the globular head
- Expression of CD46 was not downregulated by the infection of measles virus strain S191 both on mRNA level and cellular surface protein level.
- High levels of CD46 is associated with breast cancer
- Does not provide additional bbenefit in preventing graft rejection in transgenic pig-baboon heart transplantation.
- there are naturally occurring susceptibility factors in CFH and MCP for the development of atypical haemolytic-uraemic syndrome
- In transgenic pigs, has no effect on endogenous retrovirus.
- co-purification of sCD46 and HHV-6 DNA from multiple sclerosis sera indicates that HHV-6 is tightly connected to its receptor, CD46, in the serum of multiple sclerosis patients
- data support the notion that pilus-mediated gonococcal infection of epithelial cells can occur in a CD46-independent manner, thus questioning the function of CD46 as an essential pilus receptor for pathogenic neisseriae
- native conformation of the CCP2 domain is crucial for adenovirus serotype 35 binding and substitution of amino acids at positions 130 to 135 or 152 to 156 completely abolishes the receptor function of CD46
- CD46 has a role in tailoring innate immune recognition of apoptotic and necrotic cells
- The combination of homozygosity for both IL1B-511*1 and CD46H*2 alleles is a high risk factor for recurrent pregnancy loss.
- CD46 is a major cellular receptor on A549 cells for all species B adenoviruses except types 3 and 7.
- Mutations in the complement regulators factor H, membrane cofactor protein (MCP), and factor I are associated with atypical hemolytic uremic syndrome.
- the N481Y substitution in the measles virus haemagglutinin allowed it to utilize CD46 as an alternative receptor, but its ability to use CD46 was rather low in CD46+ SLAM- cell lines
- ADAM10-mediated release of CD46 from apoptotic vesicles may represent a form of strategy to allow restricted complement activation to deal with modified self
- Acts in cohort with fimbrial adhesion to promote the uptake of pathogenic Escherichia coli.
- CD3/CD46-induced T regulatory cell 1-like cells die through a process of abortive proliferation
- Ligation of CD46 on human T cells prevents recruitment of the microtubule organizing center, CD3, and perforin to the interface with the antigen-presenting cell and causes a reduction in IFN-gamma production.
- MCP mutation and complete paternal uniparental disomy of chromosome 1 could have an additive effect in determining the severity of the hemolytic-uremic syndrome phenotype.
- HHV-7 infection causes elevation of the CRPs CD46 and CD59, which may be a possible mechanism for HHV-7 to evade humoral immunity via complement.
- Emm-dependent and -independent CD46 binding of clinical isolates of Streptococci confirms the importance of CD46 as a cell target that might confer pathogens some biological advantages over the host.
- SNPs present in both the SLAM and CD46 genes are associated with measurable and significant variations in antibody response after measles vaccination
- posttransplantation outcome is poor in all complement mutation groups except for the MCP mutation group
- recurrence may be favored by vascular microchimerism, in which the mutated CD46 protein is produced in the in the kidney graft by endothelial cells originating from recipient.
- SLAM and CD46 do not have a role in measles virus growth and syncytium formation in tumor cell lines
- Membrane cofactor protein mutations in atypical hemolytic uremic syndrome (aHUS), fatal Stx-HUS, C3 glomerulonephritis, and the HELLP syndrome.
- It is concluded that the B1 and B2 species of Ad engage CD46 through similar binding surfaces.
- Measles virus can infect and produce syncytia in human polarized epithelial cell lines independently of SLAM and CD46 by using distinctive receptor-binding sites on its hemagglutinin.
- Extensive analysis of other complement regulatory protein- and polymorphism-associated risk factors did not uncover a difference between the patient and his sister.
- analysis of how species B adenovirus fiber structural variations impact CD46 association
- these results argue that bacterial binding of human CD46 enhances bacterial survival and represents a novel pathogenic mechanism that contributes to the severity of group A streptococcal disease.
- An abnormal control of the complement alternative pathway is a risk factor for the occurrence of HELLP syndrome.
- A special expression pattern and altered functions of CD46 could explain the organ-specific and virus-associated pathogenesis of otosclerosis.
- CD46-induced Tregs play a role in intestinal immune homeostasis where they dampen unwanted effector T cell responses through local IL-10 and granzyme B production.
- CD46 as a Measles virus cell entry receptor.