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Validated All-in-One™ qPCR Primer for LPL(NM_000237.2) Search again
Product ID:
HQP010847
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
HDLCQ11, LIPD
Gene Description:
lipoprotein lipase
Target Gene Accession:
NM_000237.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake.
Gene References into function
- role of Sp1 and Sp3 in interferon-gamma mediated suppression of gene transcription
- Maturation of lipoprotein lipase in the endoplasmic reticulum
- Peroxisome proliferator-activated receptor (PPARalpha and PPARgamma) agonists decrease lipoprotein lipase secretion and glycated LDL uptake by human macrophages.
- Spontaneously occurring dissociation of LPL dimer into monomers is accelerated in exon 5 mutants, resulting in conformational changes which result in loss of LPL catalytic activity.
- lipoprotein lipase cannot be a major factor in pathogenesis of Alzheimer's disease
- Low mass in preheparin serum of type 2 diabetes mellitus patients and its recovery with insulin therapy.
- results suggest that the nature of the mutation in the LPL gene modifies the relationship of HDL particle size to other metabolic variables and secondary factors such as abdominal obesity and gender.
- Variants in gene relate to presence and degree of microalbuminuria in Type II diabetes
- The S447X mutation is associated with anti-atherogenic effects on TG and HDL cholesterol in both genders, and with a moderate protective effect on risk of ischemic heart disease in men.
- LPL is bound to postprandial triglyceride-rich lipoproteins and mediates their hepatic clearance in vivo.
- molecular modeling of its dimeric structure
- variations in genes affecting the removal rate of triglycerides (TG) from plasma significantly influence the lipid phenotypic expression of familial combined hyperlipidemia
- some single-nucleotide polymorphisms in the LPL gene among Chinese associated with abnormal lipid and lipoprotein profiles and predisposition to coronary heart disease, and they are gender-specific.
- LPL has a role in atherosclerosis, chylomicronaemia, obesity, Alzheimer's disease, and dyslipidaemia associated with diabetes, insulin resistance, and infection [review]
- LPL could play a key role in the differentiation of Neuro-2A cells and in the pathophysiological effects of oxidative stress in several neurodegenerative disorders
- conclude that type IIB VLDL-1 and VLDL-2 induce triglyceride accumulation in monocyte-macrophages primarily by the lipolytic action of LPL, which may involve stabilization and activation of the enzyme, rather than modulation of enzyme production
- H+ allele of the lipoprotein lipase gene HindIII polymorphism is associated with higher plasma triglyceride and lower HDL-cholesterol levels in Chinese patients with early-onset diabetes
- LPL enzyme deficiency causes elevated plasma triglyceride level and subsequent insulin resistance; increased free fatty acids combined with insulin resistance promote gluconeogenesis and hyperglycemia, a vicious circle leading to type 2 diabetes.
- results demonstrate a direct effect of prolactin, via functional prolactin receptors, in reducing the lipoprotein lipase activity in human adipose tissue
- the LPL association with lipid profile is more likely attributable to the functional S447X rather than the nonfunctional exon 10 SNP
- The LPL D9N genotype was a significant predictor of both baseline carotid plaque area and progression. Heterozygotes for the N9 allele had higher values than did LPL D9/D9 homozygotes. D9N genotype may be a determinant of atherosclerosis.
- data indicate an important role of endoplasmic reticulum-based chaperones for the folding/dimerization of lipoprotein lipase
- LPL gene and associated regions might contribute to individual blood pressure variation and hypertension in the Chinese population
- Lipoprotein lipase gene polymorphisms might be involved in predisposition to coronary artery disease
- LPL enzyme activities in 28 healthy subjects with well-controlled Type 1 diabetes, and their relationship with Lp(A-I) and Lp(A-I,A-II)
- LPL lipolysis of emulsion triolein was retarded in chylomicron-free human plasma compared with the hydrolysis activated by isolated apolipoprotein C-II.
- LPL 44X alleles were associated with moderately increased LDL peak particle size.
- findings of variation near the LPL gene support the proposition that a region near the lipoprotein lipase gene or the lipoprotein lipase gene itself might contribute to the individual blood pressure variation in Chinese
- lipoprotein lipase has a protective role against coronary artery disease in Mexican-Americans
- did not find significant effects of lipoprotein lipase HindIII or PvuII polymorphisms on the fasting lipids but HindIII variation was associated with higher triglyceride postprandial peak
- These results by showing modulation of association between S447X variant of the LPL gene and serum TG by C-514T variant of the HL gene underscore the importance of gene-gene interactions in the assessment of genetic effects on complex traits
- Muscle can synthesize tethered, dimeric LpL, but efficient production of this enzyme leading to secretion, and physiological function appears to favor secretion of a noncovalent dimer composed of monomeric subunits.
- In Japanese, poorly controlled type 2 diabetic men had more unfavorable lipid profile than did women counterparts, which may be associated with decreased plasma LPL levels.
- results imply that systemic elevation of lipoprotein lipase expression may be potentially useful for the treatment of hyperlipidemias, obesity, and insulin resistance
- Variation in the LPL gene plays a role in determining insulin resistance in Mexican Americans.
- Macrophage LPL activity correlated with body mass index and fat mass. Incubation of patient macrophages with IGF-I for 24 h or differentiation of monocytes from GH-deficient patients into macrophages in presence of this growth factor decreased LPL.
- TRL-bound LPL activity increases in the postprandial state and is strongly reduced in type 2 diabetes, contributing to postprandial hypertriglyceridemia
- both hyperglycemia and hyperinsulinemia plus hyperglycemia reduced LPL activity to 60 %
- LPL-mediated fatty acid uptake is an inefficient process, but may be more efficient in muscle than in adipose tissue.
- Activity may explain the difference in LDL lipoprotein size in diabetic and nondiabetic people.
- Data show that human lipoprotein lipase significantly inhibited spontaneous human natural killer cells, but not lymphokine-activated killer cytotoxic activity against bovine pulmonary endothelial cells.
- X447 allele at the LPL locus is common and associated with a less atherogenic lipid profile in Asian populations
- APOC3, LPL and GpIIIa genes were found to be associated with BP levels. The contributions of these genes, although modest, are consistent with the polygenic nature of blood pressure levels.
- W86R mutation was the reason for the production of nonfunctional LPL and consequently triacylglycerol (TG) exceeding 15 mmol/l
- quantitative-transmission/disequilibrium-test analyses showed that there was linkage between DBP and two single nucleotide polymorphisms in the LPL gene
- In the ET state, only the gender difference in mLPL mRNA persisted. FAT/CD36 protein in muscle was higher in women than in men, irrespective of training status.
- In Polish LPL-H (G) allele carriers, obesity correlated with free fatty acid tolerance in males, and insulin resistance in females.
- Increased LPL activity improves insulin resistance and reduces adipose accumulation in transgenic rabbits.
- SNP analysis did not provide substantial evidence of an association between polymorphisms in the LPL gene and hypertension status and/or blood pressure levels in Chinese
- high concentration of triglyceride and/or low concentration of HDL-cholesterol are associated with high blood pressure in hypertensive patients with the X447 allele of the LPL gene.
- mean LDL particle diameter was smaller in LPL N9 carriers; the LPL N9 and S291 alleles are more frequent in CHD-free men with normal HDL-C, whereas the X447 allele is less frequent; the N9 allele is associated with the LDL subclass response to gemfibrozi
- the lipoprotein lipase X447 mutant allele may have a role in preventing myocardial infarct risk
- The lipoprotein lipase allele significantly increases the risk of developing Alzheimer's disease , and the risk is mostly associated with the H+H+ genotype.
- LPL may represent a link between low adiponectin levels and dyslipidemia in both nondiabetic individuals and patients with type 2 diabetes.
- Beta-cell LPL has two physiologically relevant effects in islets, the inverse regulation of glucose metabolism and the independent mediation of insulin secretion through effects distal to membrane depolarization.
- Single Nucleotide Polymorphisms in Lipoprotein Lipase gene is associated with variation in plasma triglyceride levels Coronary Arteriosclerosis
- The allelic frequencies of HindIII (-) and (+) allele of LPL was 18.9% and 81.1%; and of PvuII(+) and (-) allele of LPL, was 66.0% and 34.0%, respectively.
- macrophage-derived LPL in the arterial wall is pro-atherogenic, possibly via the enhancement of foam cell formation during atherogenesis.
- The polymorphisms of intron 8 in lipoprotein lipase influence the blood-lipid metabolism, induce blood vessel rebuilding and play an important role in the invasion and development of Essential Hypertension.
- Increased expression after weight loss may contribute to lower plasma ldl cholesterol and triglycerides in obese premenopausal women.
- quantification of LPL and ADAM29 gene expression is a strong prognostic indicator in CLL, providing better prognostic assessment than ZAP-70 in advanced stages of the disease
- The Expression of LPL mRNA as well as protein was highly restricted to leukemic B cells.
- A homozygote missense mutation (204 Asp (GAC)-Glu (GAG)) was found in exon 5 of the LPL gene in a hypertriglyeridemia patient. Triolein-hydrolyzing activity of this LPL was much higher than that of wild-type.
- variation in the 3' untranslated region of LPL affects LPL expression and activity, consequently influencing risk of atherosclerosis and insulin resistance
- An increase in the amount of LPL bound to LDL suggests an important mechanism to facilitate the uptake of diabetic LDL by endothelial proteoglycans and collagen in the atherosclerotic plaque.
- Data show that lipoprotein lipase suppressed tumor necrosis factor-alpha-induced gene expression, and enhanced interferon-gamma-induced gene expression, in human aortic endothelial cells.
- Plasma adiponectin appears to be a determinant of plasma triglycerides via an effect on LPL activity
- LPL variants may play a causal role in the development of hypertension in Taiwan Han Chinese
- Early recognition of severe hypertriglyceridemia in pregnancy may be caused by heterozygosity of this enzyme.
- Ca2+-dependent control of LPL dimerization might be involved in the normal post-translational regulation of LPL activity
- Enhanced TRL conversion and enhanced LDL removal combined with increased preheparin LPL concentration suggest increased enzymatic consequences as well as increased nonenzymatic consequences of LPL in LPLS447X carriers
- role for increased ligand action of LPL in LPLS447X carriers contributing to the cardiovascular protection in carriers of this mutation
- LPL-deficient subjects with hypertriglyceridemia displayed an enhanced glucose-stimulated insulin response as well as lower blood glucose levels.
- Results show that the H- allele of LPL HindIII polymorphism might have a small effect on apoB levels in the Central European Caucasian population with dyslipidemia of metabolic syndrome.
- lipoprotein lipase has roles in body composition and body fat distribution that could relate to the development of obesity or the maintenance of normal body weight
- Serum levels are lower in type 2 diabetes than in normal persons.
- results suggest that genetic variations at LPL and ApoB loci are among the factors contributing to the variability in response to lipid parameters to therapy in Type 2 diabetes.
- our results are consistent with the previously reported role of CETP and LPL genetic variants in cardiovascular risk and the possible modulation of the association between hypertension and carotid IMT by APOCIII Sst-1 variant.
- These results suggest that the homozygous initiator codon mutation rather than the heterozygous LPL-242 alteration was mainly responsible for the patient phenotypes.
- Basal bolus insulin therapyenhances preheparin LPL mass, accompanied by antiatherogenic changes in glucose and lipid metabolism in type 2 diabetes.
- No significant relationship between HDL cholesterol levels and C-514T polymorphism was found even after adjusting for age, body mass index and blood pressure.
- L252V mutation in the LPL gene is a common mutation in Chinese with familial hyperchylomicronemia syndrome
- Mitochondrial Ca2+ transfer essentially contributes to Ca(2+)-dependent maturation of LPL in the ER and its subsequent secretion during cell stimulation with an IP3-generating agonist.
- RAP binds to LPL with high affinity both in purified systems and cell extracts and RAP-deficient adipocytes secrete poorly assembled LPL
- Results suggest that LPL gene S447X polymorphism modifies the relation between central obesity and serum lipids.
- These results reveal the importance of genetic screening for LPL gene mutations D9N and S447X in a population at risk to develop hypertriglyceridemia.
- An N291S lipoprotein lipase gene polymorphism was positively associated with increased risk of venous thromboembolism.
- LPL may influence atherosclerosis risk through pleiotropic effects on each aspect of the metabolic syndrome
- HindIII and PvuII polymorphisms seem to exert a modulating role on lipid profile particularly in male type 2 diabetees, contributing to increase the risk of macrovascular events.
- LPL 447X-containing genotypes (447X+) were significantly decreased in diabetic nephropathy.
- carriers of the 447X allele displayed significantly lower TG, low-density lipoprotein cholesterol & TG/high-density lipoprotein cholesterol ratio; these findings suggest a role for the S447X polymorphism in combined hyperlipidemia
- lipoprotein lipase has a role in B-cell chronic lymphocytic leukemia
- In adipose tissue, rosiglitazone increases LPL mRNA abundance and LPL transport rate and possibly increases endothelial binding sites for LPL, but affects neither tissue LPL activity nor LPL rate of action.
- analysis of human placental lipoprotein lipase gestational and hormonal regulation
- a common polymorphism in the lipoprotein lipase gene modulates the risk level for sporadic AD in the eastern Canadian population but more importantly, indirectly modulates the pathophysiology of the brain in autopsy-confirmed cases.
- lipoprotein lipase X447 homozygotes exhibit enhanced apoB48 clearance
- The Ser447Terls polymorphism of LPL gene is significantly associated with plasma lipids and CI. G allele genotype may lead to decrease of plasma TG and increase of plasma HDL-C. G allele may be a protective genotype of cerebral infarction.
- Ser447stop and Hind III LPL polymorphisms may influence age of onset of ulcerative colitis (UC), while Hind III and Pvu II polymorphisms influence serum triglyceride in UC patients.
- suppression of either LPL or EL decreases proinflammatory cytokine expression and influences the lipid composition of THP-1 macrophages
- plasma HDL-Cholesterol differs according to lipoprotein lipase (LPL) genotypes and with dietary intake of fats
- There was no significant correlation between polymorphism of the lipoprotein lipase gene at the PvuII site and serum lipid levels in the two ethnic chinese groups, i.e., the Guangxi Hei Yi Zhuang and Han populations.
- protein kinase B, LKB1, and AMP-activated protein kinase have roles in activation of lipoprotein lipase by glucose-dependent insulinotropic polypeptide in adipocytes
- The activity of placental LPL is reduced by high levels of maternal TG and/or FFA.
- Among Asian Indians, the -T93G SNP of the LPL gene is associated with obesity but not type 2 diabetes, whereas the -G53C SNP appears to be protective against both obesity and type 2 diabetes.
- Relationship between fasting LPL activity/fat cell and fat cell size in females. In males, this relationship was seen only in the abdomen.
- no reliable ischaemic stroke associations were found with LPL T+495G (HindIII) polymorphism in individuals of Yakut ethnicity
- Combined with abdominal obesity, epistasis in the VLDL pathway (lipoprotein lipase, apolipoprotein CIII, hepatic lipase, PPARalpha, PPARgamma, and apo E genes) has a deleterious effect on fasting triglycerides and coronary artery disease risk profile
- cDNA microarray and Northern blots analysis were used to study the expression of lipoprotein lipase in lung adenocarcinoma tissues.
- EL and LPL are dysregulated in growth restricted pregnancy
- Metformin may increase LPL production, thereby increasing low density lipoprotein particle size which may be independent of the hypoglycemic effect of metforminin type 2 diabetes.
- The PvuII polymorphism cannot be used as independent genetic risk factor for coronary artery disease in patients of Saudi Arabic descent.
- Novel mutations of the LPL gene contribute to the hypertriglyceridemia observed in members of type 2 diabetic pedigrees.
- LPL HindIII and S447X polymorphisms were analyzed in 343 individuals of 66-97 years of age from a cohort of a Brazilian elderly longitudinal study and related to myocardial infarction, HDL levels, and triglyceride levels.
- lipoprotein lipase and dystrophin have roles in survival in B-cell chronic lymphocytic leukemia
- LPL could modulate HCV infectivity in vivo.
- S447X polymorphism of lipoprotein lipase is related to a decrease in plasma triglyceride in male patients with CAD, but not female patients. The presence of the S447X lipoprotein lipase polymorphism was not associated with the incidence of CAD.
- Changes in DNA methylation at adipogenic promoters during cellular aging.
- In especially Turkish women, compared to non-carriers, carriers of the LPL X447 allele have higher levels of HDL-C, LDL-C and total cholesterol, and show a degree of protection against developing the metabolic syndrome.
- Dydrogesterone and norethisterone increase secretion of LPL from abdominaal adipocytes.
- The higher lipoprotein lipase activity in lung cancer tissue provides a possible mechanism for increasing the supply of lipid nutrients to the tumor, necessary for tumor growth.
- atorvastatin reduces LPL and EL expression by reducing the activation of LXRalpha and NF-kappaB, respectively
- Circulating LPL concentrations decreased with the severity of obstructive sleep apnea syndrome
- serum concentrations of lipids did not differ between the LPL HindIII and apo E genotypes during pregnancy and after delivery. LPL PvuII SNP is associated with variations in serum lipids during pregnancy and the puerperal period in non-diabetic women.
- Data suggest that the Hind RFLP in the LPL gene is associated with T2DM risk in Chinese Han population in Hubei Province, and the H+ allele may serve as a genetic risk factor of T2DM.
- variants of apolipoprotein A-V combined with increased triglyceridemia are associated with lower lipoprotein lipase activity in vivo and with disturbances of regulation of nonesterified fatty acids
- Deletion-insertion mutation mediated by Alu repetitive elements leading to lipoprotein lipase deficiency is associated with acute pancreatitis
- Both common and rare DNA variants of lipoprotein lipase (LPL) gene were found in 10% patients with severe hypertriglyceridemia
- three common variants in the LPL gene are not an important factor in the development of Type III hyperlipidemia in probands with APOE2/2 genotype
- Lipoprotein lipase is a bridging molecule that facilitates low-density lipoprotein retention in the artery wall of transgenic mice at later stages of development of atherosclerotic aortae.
- informational spectrum method applied to identifiy evolutionary highly conserved information encoded by LPL primary structure; it was demonstrated that mutations altering LPL activity also alter this information
- Data show that the G56R substitution did not affect the ability of GPIHBP1 to reach the cell surface, nor did the amino acid substitution have any discernible effect on the binding of lipoprotein lipase, chylomicrons, or apo-AV.
- p.N318S is a major predisposing factor to hypertriglyceridaemia in the Northern Irish population
- transgenic overexpression of LPL in skeletal muscle increases cold tolerance by enhancing the capacity for fat oxidation
- During a controlled low fat (30%) diet post-absorptive vastus lateralis lipoprotein lipase activity is higher in sedentary pre-menopausal African American as compared to non-Hispanic white women.
- the polymorphic HindIII site in the LPL gene is functional because it affects the binding of a transcription factor and it also has an impact on LPL expression
- describe a case of familial LPL deficiency caused by compound heterozygosity for known (G188E) and novel (W394X) LPL gene mutations
- In insulin-resistant conditions of obesity. diabetics compared to obese subjects have increased postprandial chylomicron response and reduced adipose tissue LPL activity.
- genotype of gene LPL and B2 allele of gene CETP in patients elder 90 years are found significantly more frequently than in younger patients, that makes possible to consider they as markers of favorable course of disease and patients' long life.
- reduced adipose tissue lipoprotein lipase expression, being significantly correlated with the decrease in insulin and prolactin, suggests a role of hyperinsulinemia and hyperprolactinemia in inducing and sustaining obesity.
- Our study suggests that PvuII and Ser447Ter polymorphisms are associated with lipid profile and cerebral infarctions
- lipoprotein lipase mass in preheparin serum is lowered by the angiotensin II receptor antagonist telmisartan
- Report effects of six APOA5 variants, identified in patients with severe hypertriglyceridemia, on in vitro lipoprotein lipase activity and receptor binding.
- this study strongly suggests that two lipoprotein lipase intronic variants may be associated with development of the hypertension endophenotype with elevated plasma triglyceride(s.
- results suggest no association and interaction of lipoprotein lipase T+495G polymorphism with central obesity and serum lipids for all twin pairs
- Results suggest that the control of lipid level by LPL in the bloodstream might be an important factor in T2DM pathogenesis in the Korean population.
- biallelic inactivation of LPL by chromosomal deletion and promoter hypermethylation may play a role in human prostate cancer
- The finding that ANGPTL3 and ANGPTL4 inhibit LPL activity through distinct mechanisms indicates that the two proteins play unique roles in modulation of lipid metabolism in vivo.
- LPL 1595 C/G SNP was associated with significantly lower triglyceride levels