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Validated All-in-One™ qPCR Primer for STMN1(NM_005563.3) Search again
Product ID:
HQP010557
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
C1orf215, LAP18, Lag, OP18, PP17, PP19, PR22, SMN
Gene Description:
stathmin 1
Target Gene Accession:
NM_005563.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Gene References into function
- Decreased protein levels of stathmin in adult brains with Down syndrome and Alzheimer's disease.
- Inhibition of heat-induced phosphorylation of stathmin by the bioflavonoid quercetin
- Oncoprotein 18 levels and phosphorylation mediate megakaryocyte polyploidization in human erythroleukemia cells
- the somatic mutation identified in Op18 has profound effects on cell homeostasis that may lead to tumorigenicity
- The overexpression of this protein in poorly differentiated lung adenocarcinomas and the elevated expression of the phosphorylated forms may offer a new target for drug- or gene-directed therapy and may have potential utility as a tumor marker.
- stathmin expression is necessary for the proliferation and differentiation of early megakaryoblasts and its suppression in the later stages of megakaryocytic maturation is necessary for polyploidization.
- stathmin does not work as a pH-sensitive protein as shown by thermodynamic studies
- We report the increased expression of stathmin, a developmentally regulated tubulin-binding protein, in the brains of patients with multiple sclerosis (MS).
- These observations support a model in which downregulation of stathmin expression in megakaryocytes and other polyploid cells may be a critically important factor in endomitosis and polyploidy.
- results suggest that expression of stathmin could contribute to cancer progression/prognosis, and that stathmin may have potential as a biomarker and a therapeutic target for oral squamous cell carcinoma
- provides a biophysical basis for understanding the mechanism by which local stathmin activity gradients important for promoting localized microtubule growth
- Although Op18 is dispensable for mitosis, the hyperactive Q18-->E mutant, or overexpressed wild-type Op18, exerts aneugenic effects that are likely to contribute to chromosomal instability in tumors
- The NF-Y site at -65 accounts for greater than 60% of the Op18 gene expression.
- Stathmin repression selectively induces certain tumor-suppressor functions in cancer cells with mutant p53, including cell cycle arrest and apoptosis.
- Survivin gene promoter-driven stathmin siRNA expression vector may have potential use in tumor gene therapy with targeted tumor gene silencing effect.
- The current structural and thermodynamic studies on the tubulin-stathmin interaction were summarized.
- Loss of heterozygosity for the stathmin gene may be associated with improved outcomes of patients with chromosome 1 anaplastic oligodendroglioma tumors.
- Stathmin expression is highest when the prostate is undergoing morphogenesis or tumorigenesis and these processes may be regulated through differential phosphorylation.
- This study suggests that Epstein-Barr virus infection induces STMN1 expression, which play a role in cell cycle progression and proliferation in the human B lymphocyte.
- Overexpression of stathmin is an early protumorigenic event in human hepatocarcinogenesis, and its up-regulation can be mediated by gain-of-function mutations in p53.
- Results showed that the stathmin mRNA expression was detected in all the ovarian carcinoma tissue samples and those samples with metastasis had higher levels of stathmin mRNA expression in initial biopsy specimens.
- Depletion of Op18 by means of RNA interference increased the susceptibility of tubulin to TBCE or E-like mediated disruption, while overexpressed Op18 exerted a tubulin-protective effect.
- Stathmin plays a significant role in tumor metastasis formation.
- Op18 mediates posttranscriptional regulation of tubulin mRNA in Jurkat cells through the same basic autoregulatory mechanism as microtubule-poisoning drugs
- stathmin is probably not useful as a stand-alone marker to determine malignancy in pheochromocytomas for individual tumors
- substantial reduction in STMN1 was deminstrated upon restoration of miR-223 expression in hepatocellular cell lines
- Data found the ratio of MAP4 to stathmin mRNA was found to be higher in diseased lung tissues compared to normal lung tissues, suggesting this ratio might also be used as a clinically relevant biomarker for NSCLCs.
- Stathmin expression may be closely associated with early trophoblast migration and differentiation into syncytiotrophoblasts during placentation.
- Hyperactive melanocytes can be inhibited by altering Stathmin 1 expression.
- LMP1 regulates Op18/stathmin signaling by cdc2 mediation.