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Validated All-in-One™ qPCR Primer for LAMC2(NM_005562.2) Search again
Product ID:
HQP010537
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
B2T, BM600, CSF, EBR2, EBR2A, JEB3A, JEB3B, LAMB2T, LAMNB2
Gene Description:
laminin subunit gamma 2
Target Gene Accession:
NM_005562.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes.
Gene References into function
- cytoplasmic expression represents high invasive potential of pancreatic ductal adenocarcinoma and is correlated with distant metastasis and poorer prognosis
- laminin-gamma2 is frequently overexpressed in HNSCCs and derivative cell lines
- level of circulating LN gamma2 NH(2)-terminal fragment (G2F) is a new, prognostic, tumor-characterizing marker for estimating the invasiveness and malignancy of epithelial carcinomas
- Thus the synergistic activation of the LAMC2 gene is mediated via different cis-elements and results in an overproduction of the laminin gamma 2 chain relative to the other laminin-5 constituent chains.
- Laminin-5 gamma2 chain expression may contribute to formation of budding tumor cells at the invasive front, and immunostaining of this adhesion molecule may be useful in identifying high-risk patients for locoregional failure in T1 colorectal carcinomas.
- epidermis of the Lamc2-/- mice revealed induced apoptosis in the basal cells of the blistered skin
- Laminin gamma 2 chain exhibits aberrant expression in a stepwise manner through different aggressive stages of tumor progression.
- co-cultures of epithelial cells and fibroblasts were studied to analyse the processing of laminin 5 alpha3, beta3, and gamma2 chains
- In squamous cell carcinoma of the tongue and colorectal carcinoma, laminin 5 gamma2 chain is important in the invasiveness of cancer cells.
- Premature termination codon mutations in both alleles of LAMB3 or LAMC2 genes were found in nine of the 11 H junctional epidermolysis bullosa patients
- Data show that the expression of laminin gamma2 chain and collagen type XVII is altered in endometrial adenocarcinomas.
- up-regulation of Ang2, MMP-2, MT1-MMP, and LN 5 gamma 2 is associated with the invasiveness displayed by human gliomas
- the absolute mRNA levels generated from the laminin 5 genes do not determine the translated protein levels of the laminin 5 chains in keratinocytes; the expression of the laminin 5 genes may be controlled by common regulation mechanisms
- Ln-5 gamma2 chain regulates the secretion of the alpha3 and beta3 subunits. More importantly, suppression of Ln-5 results in a phenotype that is representative of invasive tumor cells
- This study suggests that coexpression of LN-5 gamma2 and EGFR is closely related to the progression and poor prognosis of esophageal SCC.
- Ln-5 and TGF-beta1 cooperatively induce epithelial to mesenchymal transition in hepatocellular carcinoma
- Our results suggest that PAI-1 is a novel potential marker of initial invasion in oral SCC, and that the coordinated expression of PAI-1 with uPAR and lam-gamma2 sustain the features of the early invasive cancer cells.
- Ln-5 is an important regulator of ADPKD cell proliferation and cystogenesis; Ln-5 gamma(2) chain and Ln-5-alpha(6)beta(4) integrin interaction both contribute to these phenotypic changes
- domain V of the gamma2 chain negatively regulates the integrin beta4 phosphorylation, probably through a syndecan-1-mediated signaling, leading to enhanced cell adhesion and suppressed cell motility
- findings suggest that mesenchymal cells contribute to the promotion of tumour cell migration as well as vessel formation in oral squamous cell carcinoma by providing and organising promigratory Ln-5 fragments
- Study summarizes recent progress concerning the molecular mechanisms of laminins in development and disease.
- Overexpression of Laminin-5 gamma2 is associated with oral squamous cell carcinomas
- The TP0136 protein was exposed on the surface of the bacterial outer membrane and bound to the host extracellular matrix glycoproteins fibronectin and laminin.
- LAMC2 mRNA was overexpressed in 89% of esophageal squamous cell carcinoma cases.
- the alpha3 chain can assemble with only beta3-gamma2 heterodimer to form a heterotrimer via disulfide bonds
- The expression of LAMC2-COL4A1 genes were particularly effective in distinguishing OSCC from normal oral tissue.
- Laminin isoforms containing the gamma3 chain are unable to bind to integrins due to the absence of the glutamic acid residue conserved in the C-terminal regions of the gamma1 and gamma2 chains
- Results suggest that HIF1 contributes to keratinocyte migration and thus to the re-epithelialisation process by regulating laminin-332.
- proteolytic processing of the LN-5 gamma 2 chain by MT1-MMP is involved in cell migration and subsequent architectural organization in ovarian clear cell carcinoma
- LAMC2 is a novel biomarker of bladder cancer metastasis that reflects the propensity of cells to metastasize via either lymphatic or hematogenous routes.