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Validated All-in-One™ qPCR Primer for L1CAM(NM_000425.4) Search again
Product ID:
HQP010390
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CAML1, CD171, HSAS, HSAS1, HYCX, MASA, MIC5, N-CAM-L1, N-CAML1, NCAM-L1, S10, SPG1
Gene Description:
L1 cell adhesion molecule
Target Gene Accession:
NM_000425.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation.
Gene References into function
- genetic and clinical aspects of X-linked hydrocephalus (L1 disease)
- May be a modifier of a gene associated with Hirschsprung disease.
- Hirschsprung disease and L1CAM: is the disturbed sex ratio caused by L1CAM mutations?
- A novel missense mutation in the L1CAM gene is identified in a boy with X-linked hydrocephalus who had multiple small gyri, hypoplasia of the white matter, agenesis of the corpus callosum, and lack of cleavage of the thalami.
- L1CAM might contribute to melanoma progression by promoting cell adhesion and migration.
- X-linked hydrocephalus is a variable condition caused by mutations in the gene encoding for L1CAM. This gene is located at Xq28.
- This review focuses on the L1CAM extracellular region and how recent work has clarified important aspects of its structure and function; new insights are provided for L1CAM binding to extracellular molecules, and how L1CAM initially folds.
- Data show that L1-cell adhesion molecule interactions with ankyrinB (but not with ankyrinG) are involved in the initial formation of neurites.
- L1-type CAMs are able to promote the adhesion-dependent activation of EGF receptor signaling in vitro and in vivo
- This study provides a novel translational mechanism to account for the association between L1 expression and motile processes involved in metastasis and development.
- L1CAM may be a modifying factor in the development of Hirschsprung's disease
- The sixth Ig-like domain of L1 (L1Ig6) demonstrates angiogenic potential involving ligation and activation of alpha(v)beta3.
- L1 expression conferred increased cell motility, growth in low serum, transformation and tumorigenesis.
- Levels of CAM-L1 were increased in the prefrontal cortex of the major depression.
- RanBPM is an adaptor protein that links L1 to the extracellular signal-regulated kinase/MAPK pathway.
- The cleavage of the ectodomains of L1 and CD44 is initiated in an endosomal compartment that is subsequently released in the form of exosomes.
- Data show that L1 is highly expressed in gastrointestinal stromal tumors but not in leiomyoma and desmoid-type fibromatosis being important differential diagnoses.
- L1CAM has a function in synapse formation by organizing microtubules in the synaptic terminal.
- L1 cell adhesion molecule plays a critical role in melanoma invasion and progression and offers therapeutic potential in combination with conventional anticancer agents.
- High L1 expression is associated with colorectal cancer progression
- A LiCAM mutation was presented in a boy with hydrocephalus and duplex kidneys.
- L1CAM loss-of-function mutations cause a severe form of L1 syndrome.
- ADAM10 overexpression in colon cancer cells displaying endogenous L1-CAM enhanced L1-CAM cleavage and induced liver metastasis, and ADAM10 also enhanced metastasis in colon cancer cells stably transfected with L1-CAM.
- a significant association of L1 expression and presence of disseminated tumor cells colorectal tumors
- Expression of L1-CAM augments cell motility, invasiveness and tumor growth, causes sustained Erk kinase activation and augments transcriptional activity of proinvasive genes.
- A soluble form of human L1 can be detected in the urine of patients with acute tubular necrosis and this may be a marker of distal nephron injury.
- Alpha v beta 5, alpha v beta 5 and their ligands Del-1 and L1 play an important role in the process of tumor cells moving from the original place.
- We propose that L1CAM could promote endometriosis development by increasing enervation and aggravation. L1CAM expression is higher in atypical endometriosis compared with normal endometriosis.
- L1 (CAM) expression represents a novel diagnostic marker in serous ovarian neoplasms that show characteristics of tumor progressio; immunoreactivity correlated significantly with stage and grade
- ALCAM coordinates tissue growth and cell migration in a process involvnig L1CAM
- Importance of the RGD site in L1 for human tumors; nuclear signaling of L1 is dependent on integrins.
- L1CAM is required for maintaining the growth and survival of CD133(+) glioma cells both in vitro and in vivo, and L1CAM may represent a cancer stem cell-specific therapeutic target for improving the treatment of malignant gliomas and other brain tumors
- domains Ig1 to Ig4 are necessary and sufficient for L1 homophilic binding in trans, and that the rest of the molecule does not contribute to the affinity
- L1-CAM has a role in metastasis in colon cancer cells [review]
- L1 expression indicates more mature stages of neuroblastoma and is associated with less aggressive tumor cell behavior..
- Using shotgun mass spectrometry, we found this protein differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia.