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Validated All-in-One™ qPCR Primer for MAFA(NM_201589.3) Search again
Product ID:
HQP010383
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
INSDM, RIPE3b1, hMafA
Gene Description:
MAF bZIP transcription factor A
Target Gene Accession:
NM_201589.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).[supplied by OMIM].
Gene References into function
- MafA selectively induces endogenous insulin transcription in non-beta cells
- mafA has a role in regulating insulin gene expression in the liver
- MafA plays a key role in coordinating and controlling the level of insulin gene expression in islet beta cells.
- Co-expression and functional synergism of these beta-cell enriched transactivators, MafA, Pdx1, and Beta2, are critical for establishing the beta-cell-specific and efficient expression of the insulin gene.
- Data show that FoxA2, Nkx2.2, and PDX-1 regulate islet beta-cell-specific mafA expression through conserved sequences located between base pairs -8118 and -7750 upstream from the transcription start site.
- MafA has a critical role in islet beta-cell function
- Transcriptional and transforming activities of MafA are positively regulated by GSK-3-mediated phosphorylation.
- Under diabetic conditions, expression and/or activities of PDX-1 and MafA in beta-cells are reduced [REVIEW].
- Significance of large Maf proteins to Pdx1 expression in beta cells, and in particular MafB during pancreatic development.
- Large Maf proteins (MafA, MafB and c-Maf) are implicated in human cancers.
- Data suggest that a group of transcription factors, including MafA and Foxa2, regulate expression of two major autoantigens in type 1 diabetes, including islet-specific glucose-6-phosphatase catalytic subunit-related protein.
- MafA levels are tightly controlled by the coordinated action of multiple kinase pathways