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Validated All-in-One™ qPCR Primer for KRT19(NM_002276.4) Search again
Product ID:
HQP010233
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CK19, K19, K1CS
Gene Description:
keratin 19
Target Gene Accession:
NM_002276.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq].
Gene References into function
- Cyfra 21-1 fragment is a sensitive marker in the diagnosis of distant metastases of head and neck carcinoma
- CK19 expression is a predictor of early postoperative recurrence due to increased invasiveness
- The results demonstrate that overexpression of CK19 in hepatocellular carcinoma cells is related to metastatic behavior.
- CK19 expression is significantly higher in thyroid papillary carcinoma than benign thyroid tissue; and this characteristic can have important diagnostic value.
- RET/PTC and CK19 have roles in progression of papillary thyroid carcinoma
- SLUG levels in the cell regulated the function of cytokeratins 8 and 19 gene promoters.
- Cytokeratin 19 is highly expressed in HER-2/neu-positive breast tumors when compared with HER-2/neu-negative breast tumors
- High levels of serum CYFRA21-1 were correlated with the progression of thymic carcinoma
- The gene expression profile of hepatic stem cells throughout life consists of high levels of expression of cytokeratin 19 (CK19).
- The large Paget cells uniformly expressed CK19 in 12/12 extramammary Paget's disease.
- Intensive follow-up by serial assay of CEA and cytokeratins (8, 18, 19) allows early detection of colorectal neoplasm recurrence.
- The higher recurrence rate of CK19+ HCC (hepatocellular carcinomas) after transplantation suggests a worse prognosis for these HCCs compared with CK19- HCC.
- [in] serum an independent prognostic factor for oral squamous cell carcinoma
- Molecular staging of SLN using real-time RT-PCR for early breast cancer could serve as a useful complement to standard clinicopathological risk factors.
- In conclusion, CYFRA 21-1 is a useful tumor marker before and after surgical treatment in lung cancer.
- Detection of CK19 mRNA in the bone marrow of breast cancer patients is an independent predictor of relapse-free survival in operable breast cancer patients.
- KRT19 variants are not overtransmitted or associated with familial IBD, although a potential role in sporadic IBD cannot be excluded.
- Diffuse-type gastric cancer tissues at varying purities, were evaluated for the expression of a cancer-spec. gene, KRT19, by RT-PCR. Tissues of 70% purity for cancer cells, by microdissection, were of sufficient quality to proof the KRT19 gene expression
- CK19/CD99 immunoexpression did not correlate with extent of tumor invasion, mitotic activity, Ki-67 labeling index, presence of extracellular mucinous pools dissecting muscle, and angiolymphatic and perineural/neural invasion in goblet cell carcinoids.
- serum Sialyl Lewisx and cytokeratin 19 fragment were prognostic markers for stage I non-small cell lung cancer
- Comparing follicular variant of papillary carcinoma (FVPC) with follicular carcinoma (FC), the expression CK19 was significantly higher in FVPC.
- There was a highly significant difference between overall survival time and lymph node metastasis with LOH and CK19 analysis.
- A high serum CYFR21-1 concentration is associated with tumor progression and poor postoperative outcomes in patients with intrahepatic cholangiocarcinoma.
- CK19 staining is a potential additional prognostic marker independent from the WHO criteria for pancreatic endocrine tumors.
- Our results suggest that the degree of cytokeratin 19 expression could be a new prognostic factor in non-small cell lung cancer.
- CK 19 expression can be helpful in differentiating sebaceous tumors from basal cell carcinomas.
- Data indicate that expression of CK19 by qPCR is a quantitative method for distinguishing papillary carcinoma lesions from other types of thyroid lesions, in contrast to the more qualitative immunohistochemistry.
- This preliminary study provides evidence that the CK19/EpCAM2 and/or CK19/P-cadherin ratio(s) may be a simple and accurate prognostic indicator of clinical outcome in early-stage adenocarcinoma of the lung.
- Cyfra 21.1 may be regarded as a circulating marker of poorly differentiated and anaplastic thyroid carcinoma.
- The detection of peripheral blood CK19mRNA+ and MGB1mRNA+ cells before adjuvant chemotherapy predicts poor DFS in women with early breast cancer.
- CK10 alone, or in combination with CK19, can be a novel predictor for poor prognosis of hepatocellular carcinoma patients after curative resection.
- Distinct cytokeratin 7, cytokeratin 19, & neuronal cell adhesion molecule staining patterns are seen in hepatic adenoma & focal nodular hyperplasia possibly suggest activation of different subsets of hepatic progenitor/stem cell.
- Micrometastases of gastric cancer can be detected in circulating peripheral blood using quantitative real-time RT-PCR. CK19 is a better marker than CK18, CK20 and CEA.
- Coexistence of strong CK19 positivity and p63-positive cells can enhance the cytologic diagnosis of PTC.
- gene expression of krt8, krt18, and krt19 and correlation with gene expression profiles and cell differentiation are compared in human and mouse embryonic stem cells