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Validated All-in-One™ qPCR Primer for KCNJ6(NM_002240.4) Search again
Product ID:
HQP010010
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
BIR1, GIRK-2, GIRK2, KATP-2, KATP2, KCNJ7, KIR3.2, KPLBS, hiGIRK2
Gene Description:
potassium inwardly rectifying channel subfamily J member 6
Target Gene Accession:
NM_002240.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses.
Gene References into function
- glutamate residue at the C terminus regulates activity -- implications for Andersen Disease (inward rectifier potassium channel 2; IRK2)
- L344 and G347 play an important functional role in G(betagamma) activation of GIRK2 channels.
- The potential synergy and consequences of the overexpression of KIR3.2 and KIR4.2 in Down's syndrome brain development are discussed.
- GIRK channels are important functional effectors of the P2Y(12) receptor in human platelets.
- Decreased GRK 2 expression most likely results from reduced cAMP stimulation in cold thyroid nodules.
- GIRK1 and GIRK2 channels, but not GIRK3 or GIRK4, may may activate signaling pathways in development of lung cancer
- These data suggest that KCNJ6 could play an important role in altered cardiac regulation in Down syndrome patients.