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Validated All-in-One™ qPCR Primer for AREG(NM_001657.3) Search again
Product ID:
HQP009941
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
AR, AREGB, CRDGF, SDGF
Gene Description:
amphiregulin
Target Gene Accession:
NM_001657.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, and fibroblasts.
Gene References into function
- (TARP) expression in prostate cancer cells leads to an increased growth rate and induction of caveolins and amphiregulin.
- mRNA expression in atrophic gastritis before and after Helicobacter pylori eradication
- H358 cells secrete a high level of amphiregulin that, in combination with IGF1, prevents serum deprivation apoptosis
- Review. The activation of EGFR in response to smoke involves cleavage of amphiregulin by ADAM 17.
- amphiregulin binds to EGF receptor and has a role in stimulation of lung epithelial cells upon exposure to tobacco smoke
- Data show that in squamous cell carcinoma cells, stimulation with G protein-coupled receptor agonists specifically results in cleavage and release of amphiregulin (AR) by TACE.
- Amphiregulin(AR) overexpressing cells (HaCaT-AR) displayed autonomous proliferation in serum-free media. HaCaT-AR cells formed rapidly growing tumors with AR expression similar to keratoacanthoma.
- epidermal AR expression as a possible mediator of innate cutaneous immunity and epidermal proliferation and also as a potential trigger of both cutaneous psoriasis and psoriatic arthritis.
- amphiregulin- and ErbB1-dependent mechanism by which autocrine ERK activation is maintained in normal keratinocytes
- AR is under strong regulation by the cAMP pathway in various cell types
- upregulation of the epiregulin and amphiregulin expression is part of the signal transduction pathway which leads to ovulation and luteinization in the human ovary
- an early-response growth factor that may contribute to the initial phases of liver regeneration.
- Amphiregulin was secreted by human mast cells after aggregation of FcepsilonRI. Amphiregulin may be new target molecule for treatment of overproduction of sputum in bronchial asthma.
- Mast cells secreted amphiregulin on IgE cross-linking, and amphiregulin induced proliferation of lung fibroblasts. Local release of amphiregulin by mast cells could play role in lung fibrosis by promoting proliferation of lung fibroblasts.
- AREG gene expressed by purified primary myeloma cells, and expression higher than in normal bone marrow (BM) plasma cells or plasmablastic cells; AREG plays an important role in biology of multiple myeloma
- AR and IGF1 cooperate to prevent apoptosis by activating a specific PKC-p90(rsk)-dependent pathway, which leads to Bad and Bax inactivation.
- Enhanced transmigration of human neutrophils through polarized epithelial cell monolayers of MDCK cells after administration of AR, further supporting aspecific role for AR in pathogenesis of psoriasis.
- Data suggest that parathyroid hormone-induced amphiregulin mRNA expression is mediated primarily through cyclic AMP-protein kinase A-CREB signaling.
- Results suggest that hypoxia promotes intestinal epithelial amphiregulin expression in a CREB-dependent manner, an event that may contribute to increased proliferation.
- The mechanism of deoxycholic acid-induced epideermal growth factor receptor activation is ligand-dependent and is controlled, at least in part, at the level of amphiregulin release from the basolateral cell membrane.
- HAT induces amphiregulin production through the PAR-2 mediated ERK pathway, and then causes amphiregulin release by a TACE-dependent mechanism
- Amphiregulin and HB-EGF mediate retinoic acid-mediated epidermal hyperplasia
- results show that TACE undergoes phosphorylation that regulates release of amphiregulin upon GRP treatment; a signaling cascade of GRP-Src-PI3-K-PDK1-TACE-amphiregulin-EGFR with multiple points of interaction, translocation & phosphorylation is suggested
- findings demonstrated that PGE2 may mimic LH action at least in part by the activation of amphiregulin and epiregulin biosynthesis in human granulosa cells
- Amphiregulin (AR) up-regulates a number of genes involved in cell motility and invasion in MCF10A cells, suggesting that an AR autocrine loop contributes to the aggressive breast cancer phenotype.
- AREG is up-regulated in tumor cells of SCC but not BCC of the skin. AREG is also overexpressed in asymptomatic epidermis adjacent to both SCC and BCC, relative to normal skin.
- MIP-3alpha-mediated ERK1/2 activation in Caco-2 cells appeared to require metalloproteinase-dependent release of the endogenous EGFR ligand amphiregulin and transactivation of the EGFR.
- After exposure to cisplatin, the resistant breast cancer cells secrete amphiregulin protein by short interfering RNA resulting in a nearly complete reversion of the resistant phenotype.
- TNFalpha may play a key role in cooperation with HB-EGF and AREG in the proliferation of epidermal keratinocytes at the psoriatic skin lesions.
- AREG is preferentially expressed in breast tumors co-expressing HER2/HER4. AREG is associated with estradiol receptors, small tumour size, low histoprognostic grading, high HER4 levels.
- amphiregulin-specific EGF-R fate results from decreased hSprouty2 degradation and reduced Cbl recruitment to underphosphorylated EGF-R, two effects that impair EGF-R trafficking to lysosomes
- serum amphiregulin in patients with advanced non-squamous, non-small cell lung cancer
- Amphiregulin has a protective effect against apoptosis in the human corpus luteum.
- plasma-membrane-anchored growth factor pro-amphiregulin binds A-type lamin and regulates global transcription
- interleukin-4 and amphiregulin have roles in the proliferation of human airway smooth muscle cells and cytokine release