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Validated All-in-One™ qPCR Primer for KCNA2(NM_004974.3) Search again

Product ID:
HQP009865 (click here to view gene annotation page)
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Species:
Human
Symbol:
KCNA2
Alias:
DEE32, EIEE32, HBK5, HK4, HUKIV, KV1.2, MK2, NGK1, RBK2
Gene Description:
potassium voltage-gated channel subfamily A member 2
Target Gene Accession:
NM_004974.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
 
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Buy Catalog# Description Note Price (US$)
HQP009865 All-in-One™ qPCR Primer for NM_004974.3 (200 reactions) KCNA2 inquire
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HQP006940 All-in-One™ qPCR Primer for NM_002046.7 (200 reactions) Reference Gene GAPDH inquire
HQP015171 All-in-One™ qPCR Primer for NM_004048.3 (200 reactions) Reference Gene B2M inquire
HQP015171 All-in-One™ qPCR Primer for NM_004048.4 (200 reactions) Reference Gene B2M inquire
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Validated result:

AMP Melt
A B
Figure: All-in-One™ qPCR Primer Validated result to KCNA2.

Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve.

Summary

Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s).


Gene References into function