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Validated All-in-One™ qPCR Primer for ITGB4(NM_000213.4) Search again
Product ID:
HQP009819
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD104, GP150, JEB5A, JEB5B
Gene Description:
integrin subunit beta 4
Target Gene Accession:
NM_000213.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq].
Gene References into function
- Reduction in integrin alpha6beta4 is related to changes seen during immortalization and malignant progression
- Acute hyperglycaemia induces an up-regulation of seven major genes, four of which were not previously reported in the literature. Northern blot analyses, performed on these 4 genes, confirm macroarrays results for alphav, beta4, c-myc, and MUC18.
- binding of beta 4 to plectin is essential for the proper formation and function of hemidesmosomes
- The integrin beta4B and beta4C variants were found not to be expressed in a tissue-specific manner.
- Integrin (alpha 6 beta 4) regulation of eIF-4E activity and VEGF translation (Integrin alpha6beta4)
- integrin beta-4 expression in thyroid carcinoma may play a role in the development of gross lymph node metastasis of papillary carcinomas.
- integrin beta4 expression is increased when ROR alpha is activated in prostate cancer cells
- Data suggest that the stability of the interaction between alpha6beta4 and laminin-5 is influenced by the clustering of alpha6beta4 through the deposition of laminin-5 underneath the cells.
- Results suggest that alpha6beta4 integrin-mediated interactions of astrocytes with beta(ig)-h3 transduce intracellular signals through the focal adhesion proteins, which may regulate certain aspects of astrocyte response to brain injury.
- This study revealed detailed topography of integrins in malignant tumours derived from intercalated acinar segment of salivary gland which might be useful their diagnosis , especially of fine-needle aspiration products or from incisional biopsy.
- extracellular domain of beta 4 integrin, through its ability to bind ligand, functions to integrate the divergent effects of growth factors on the cytoskeleton and adhesion receptors
- Data show that macrophage stimulating protein (MSP) and its receptor (Ron) induce phosphorylation of both Ron and alpha6beta4 integrin, and result in activation of alpha3beta1 integrin.
- integrin beta4/calcium-activated chloride channel ligation elicits selective signaling via focal adhesion kinase to promote metastatic growth
- compartmentalization in lipid rafts is necessary to couple the alpha6beta4 integrin to a palmitoylated src family kinases and promote EGF-dependent mitogenesis.
- Data demonstrate that alpha6beta4 integrin mediates pancreatic epithelial cell adhesion to Netrin-1, whereas recruitment of alpha6beta4 and alpha3beta1 regulate the migration of putative pancreatic progenitors on Netrin-1.
- Pyloric atresia-junctional epidermolysis bullosa syndrome is caused by mutations in this gene in a case report.
- alpha(6)beta(4) has a generic influence on the invasion of carcinoma cells that is not specific to Met
- Results suggest that bacterial heat shock protein 60 may promote epithelial cell migration through activation of epidermal growth factor receptor and mitogen-activated protein kinases, and inhibition of alpha6beta4 integrin expression.
- cAMP-Epac-Rap1 pathway regulates cell spreading and cell adhesion to laminin-5 through the alpha3beta1 integrin but not the alpha6beta4 integrin
- link between the ligation of the beta(4) integrin and beta(1) integrin-mediated cell adhesion in carcinoma and pre-carcinoma cells.
- high expression of HPV type 8 (HPV8) E2 in cultured primary keratinocytes leads to strong down-regulation of beta4-integrin expression levels
- Blockage of beta4 integrin activity by antagonizing antibody abrogated ATV-induced endothelial death.
- activation by E-selectin, thus modulating adhesion
- These results provide genetic evidence that alpha6beta4 signaling promotes the onset of the invasive phase of pathological angiogenesis
- alpha6beta4 can augment cell-cell adhesion and slow down haptotaxis over laminin-5
- The beta4 integrins is a functional connection between CD9, as evidenced by anti-CD9 antibody effects on beta4-dependent cell spreading.
- immunohistochemistry clearly demonstrated colocalisation between calcium activated chloride channel 2 and integrin beta4
- the CH1 domain of the plectin-ABD associates with the groove between the two FNIII domains of beta4
- beta4ctd- form is lost from colon cancer cells, while the level of the wild-type form of beta4, which is functional for adhesion to laminin, is increased in primary tumors in relation with the expression of c-Myc
- alpha6beta4 integrin is mobilized and activated durign carcinoma progression [review]
- PMP22 forms a complex with alpha6beta4 integrin in cultured colonic adenocarcinoma cells.
- EWI proteins EWI-2 and EWI-F, alpha3beta1 and alpha6beta4 integrins, and protein palmitoylation have contrasting effects on cell surface CD9 organization
- Slug regulates integrin alpha3, beta1, and beta4 expression and cell proliferation in human epidermal keratinocytes
- The results confirm the relevance of beta4 expression in mammary tumors and indicate this integrin as a relevant target for tumor therapy.
- Ln-5 is an important regulator of ADPKD cell proliferation and cystogenesis; Ln-5 gamma(2) chain and Ln-5-alpha(6)beta(4) integrin interaction both contribute to these phenotypic changes
- Both the beta4 integrin ligand-binding and cytoplasmic domains together with epidermal growth factor were required for the synergistic activation of a Rac-dependent signaling pathway that was essential for keratinocyte directional migration.
- The role of PTHrP in breast cancer growth and metastasis may be mediated via upregulation of integrin alpha6beta4 expression and Akt activation, with consequent inactivation of GSK-3.
- domain V of the gamma2 chain negatively regulates the integrin beta4 phosphorylation, probably through a syndecan-1-mediated signaling, leading to enhanced cell adhesion and suppressed cell motility
- Data show that in bullous pemphigoid, expression of beta4 integrin was irregular and was detected mainly in dermal part of the blister.
- analysis of the signaling functions of the beta4 integrin intracellular domain
- integrin beta4 might mediate vascular endothelial cell senescence through depressing Ca(2+)-independent phosphatidylcholine-specific phospholipase C
- Increase in ITGB4 is associated with lymph node metastasis in squamous cell carcinoma of the tongue
- the alpha6beta4 integrin strongly influence Akt phosphorylation through ErbB-3 protein regulation
- beta4 expression in human breast cancer is restricted and associated with basal-like cancers
- A novel isochroman derivative inhibited apoptosis in vascular endothelial cells through depressing the levels of integrin beta4 and TP53.
- Deletion of the first pair of fibronectin type III repeats of the integrin beta-4 gene is associated with epidermolysis bullosa, pyloric atresia and aplasia cutis congenita in the original Carmi syndrome patients.
- ITGB4 SNP seems to influence tumour aggressiveness and survival in breast cancer patients.
- beta4 interacts with the extracellular environment through both stable and transient interactions and may be managed through a different endosomal trafficking pathway than alpha3.
- ZKSCAN3 regulates the expression of several genes favoring tumor progression including integrin beta 4 and VEGF
- ITGB4 mutation may induce a desquamative enteropathy in infancy without significant skin disease.
- integrin beta4 is implicated in and associated with p53 in autophagy of lung cancer cells
- Itgb4 protein remains expressed in the early stages of peripheral regeneration and subsequent myelination.
- results identify Y1494 as a major regulatory site for signaling from the alpha6beta4 integrin to promote tumor development and progression
- These data suggest an important role for beta4 integrin S(1424) in the gradual disassembly of hemidesmosomes induced by cell retraction.
- The data suggested that autophagy played a key role in Cd2+ induced endothelial dysfunction; integrin beta4, caveolin-1 and PC-PLC might be the targets of Cd2+ in vascular endothelial cells.
- Integrin alpha6beta4 and NET1 coexpression is independently associated with the development of distant metastasis in breast cancer.
- Curcumin inhibits breast cancer cell motility and invasion by directly inhibiting the function of alpha(6)beta(4) integrin, and suggest that curcumin can serve as an effective therapeutic agent in tumors that overexpress alpha(6)beta(4).