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Validated All-in-One™ qPCR Primer for ITGB3(NM_000212.2) Search again
Product ID:
HQP009818
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
BDPLT16, BDPLT2, BDPLT24, CD61, GP3A, GPIIIa, GT, GT2
Gene Description:
integrin subunit beta 3
Target Gene Accession:
NM_000212.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq].
Gene References into function
- Distinct domains of alphaIIbbeta3 support different aspects of outside-in signal transduction and platelet activation induced by LSARLAF.
- Both the high affinity thrombin receptor (GPIb-IX-V) and GPIIb/IIIa are implicated in expression of thrombin-induced platelet procoagulant activity.
- A naturally occurring point mutation in the beta3 integrin MIDAS-like domain affects differently alphavbeta3 and alphaIIIbbeta3 receptor function.
- Lateral clustering of platelet GP Ib-IX complexes leads to up-regulation of the adhesive function of integrin alpha IIbbeta 3
- Review. Alpha(v)beta3 integrins are involved in vascular repair processes. The challenge is to develop a therapeutic agent that will prove effective in reducing restenosis in humans following percutaneous coronary intervention (PCI).
- GPIIb-IIIa complexes are not in an activated conformational state after dissociation of abciximab unless there is an additional source of activation.
- beta3-Integrin expression was directly up-regulated by HOXA10 in the endometrium
- location was observed on or near the cell surface suggesting it might participate in surface membrane transport of iron
- A novel 196Leu to Pro substitution in the beta3 subunit of the alphaIIbbeta3 integrin in a patient with a variant form of Glanzmann thrombasthenia prevents GPIIbIIIa from binding fibrinogen when platelets are activated.
- Unique ability of integrin alpha(v)beta 3 to support tumor cell arrest under dynamic flow conditions (Integrin alpha-v beta-3)
- Coordinate interactions of Csk, Src, and Syk kinases with [alpha]IIb[beta]3 initiate integrin signaling to the cytoskeleton
- Del1 mediates vascular smooth muscle cell adhesion, migration, and proliferation through interaction with integrin alpha(v)beta(3).
- Immunohistochemical analysis of human tumor sections from several organs showed a heterogeneus distribution of CD61 in metastatic cases from colon and breast carcinoma. However, no staining was found in metastasis from melanoma.
- Relationships between Rap1b, affinity modulation of integrin alpha IIbbeta 3, and the actin cytoskeleton (integrin alpha(IIb)beta(3))
- Site-directed mutagenesis of residues on the top surface of the integrin beta3 I-domain that contains a putative ligand binding site identified D158 and N215 as novel residues critical for ligand binding.
- Factor XIII mediates adhesion of platelets to endothelial cells through alpha(v)beta(3) and glycoprotein IIb/IIIa integrins.
- A new platelet polymorphism Duv(a+), localized within the RGD binding domain of glycoprotein IIIa, is associated with neonatal thrombocytopenia
- collagen receptor glycoprotein VI and alphaIIbbeta3 trigger distinct patterns of receptor signalling in platelets, leading to tyrosine phosphorylation of PLCgamma2 (integrin alphaiibbeta3)
- Integrin alpha IIbbeta 3 has agonist-specific activation states and causes intrasubunit and intersubunit allosteric effects
- Two different beta3 cysteine substitutions alter alphaIIb beta3 maturation and result in Glanzmann thrombasthenia.
- Integrin-linked kinase transiently associates with and phosphorylates beta3, regulating platelet integrin alphaIIb beta3 in a PI3-kinase dependent manner
- Alpha(v)beta(3) integrin engagement enhances cell invasiveness in human multiple myeloma.
- integrin alphaVbeta3 expression is reduced when ROR alpha is activated in prostate cancer cells
- Integrin alphavbeta3. Expression of integrin alpha v beta 3 promotes the metastatic phenotype in human melanoma by supporting specific adhesive, invasive and migratory properties of the tumor cells
- Disruption of the long-range GPIIIa Cys(5)-Cys(435) disulfide bond results in the production of constitutively active GPIIb-IIIa (alpha(IIb)beta(3)) integrin complexes.
- Role for beta3 integrins in human melanoma growth and survival.
- alpha v beta 3 integrin signaling through Shc recruitment in response to mechanical stimulation in human osteoblasts
- regulation of integrin function by CD47 ligands: differential effects on integrin-mediated adhesion
- phosphoinositide 3-kinase C2alpha was found to be differentially regulated by alpha(v)beta(3) engagement
- promotion of integrin alpha Vbeta 3-dependent endothelial cell adhesion by PGE2
- co-stimulation of G(12/13) and G(i) pathways is sufficient to activate GPIIb/IIIa in human platelets in a mechanism that involves intracellular calcium
- Data show clearly that integrin alpha(v)beta(3) interacts with the latency-associated peptide (LAPbeta1 and 3) RGD motif.
- role in mediating pro-angiogenic activity of CYR61
- An autocrine loop of the integrin alpha(V)beta(3)/CD47 receptor complex and thrombospondin-1 is identified as the molecular coupling device between mechanical loading and apoptosis.
- in patients with endometriosis a significant negative correlation was observed between luminal expression of eNOS and alpha(v)beta(3) integrin and between glandular expression of eNOS and luminal expression of alpha(v)beta(3) integrin
- thrombin interacts with alphavbeta3 as demonstrated by direct binding of alphavbeta3 protein to immobilized thrombin.
- a 17-amino acid sequence is identified as a common epitope for antibodies associated with quinine-induced immune thrombocytopenia
- activation state of alphaVbeta3 integrin is an important regulator of the duration of insulin-like growth factor I receptor phosphorylation and this regulation is mediated through changes in the subcellular localization of SHP-2
- Ligand binding promotes the entropy-driven oligomerization of this protein
- Data show a novel mechanism by which ECM regulates membrane-type 1 matrix metalloproteinase (MT1-MMP) association with beta1 or alphavbeta3 integrins, thus modulating its internalization, activity, and function on human endothelial cells.
- role of Pro33 polymorphism in enhanced activation of mitogen-activated protein kinase and myosin light chain kinase
- integrin alpha5beta1-mediated control of the levels of integrin alphaVbeta3 is important for the distribution of focal contacts
- Integrin alphaiibbeta3 plays a role in signal transduction that leads to a defect in leukocyte adhesion
- has a role in the early phase of liver metastasis
- possible association between the GPIIb/IIIa PIA1/A2 polymorphism and the occurrence of cryptogenic stroke in young patients
- data suggest that despite partial disruption of calf-1 or calf-2 domain by mutation in Glanzmann thrombasthenia, glycoprotein IIb/IIIa complex is formed but its transport from the endoplasmic reticulum is impaired
- Review. A signal is initiated at the cytoplasmic tail to transform the extracellular domain of alphaIIbbeta3 into a functional receptor for fibrinogen or von Willebrand factor to support platelet aggregation & thrombus formation.
- thymidine phosphorylase and 2-deoxyribose-induced focal adhesion kinase phosphorylation was blocked by the antibodies to integrins alpha 5 beta 1 and alpha v beta 3, directly linking their migration and signaling components
- decreased expression of endometrial integrin alphavbeta3 suggests that functional, but not morphological, endometrial defect may be one of the causes for the patients with unexplained infertility and recurrent IVF-ET failures
- Alphavbeta3 integrin expression and pinopode formation in human endometrium are processes closely related to endometrial maturation; unlikely as targets for contraceptive approaches or fertility-promoting strategies
- Intercellular calcium communication is primarily mediated by a signaling mechanism operating between this protein, ITGA2B and the adenosine diphosphate purinergic receptor P2Y12.
- tumstatin binds to alpha v beta 3 integrin in a vitronectin/fibronectin/RGD cyclic peptide independent manner
- in megakaryocytes turbulence induces Rap1 activation that controls alphaIIbbeta3-mediated cell adhesion
- role of polymorphisms in cardiovascular thrombotic disease [review]
- Data suggest that this alphavbeta3 binding to alpha5-laminins is involved in the regulation of cellular responses to growth factors known to be involved in epithelial and endothelial development.
- This study revealed detailed topography of integrins in malignant tumours derived from intercalated acinar segment of salivary gland which might be useful their diagnosis , especially of fine-needle aspiration products or from incisional biopsy.
- binding of thrombin to GPIbalpha induces fibrin binding to resting alphaIIbbeta3 leading to fibrin-dependent platelet aggregation and clot retraction, that can be selectively inhibited by alphaIIbbeta3 antagonists
- results suggest that homomeric associations involving transmembrane domains provide a driving force for integrin activation; results also suggest a structural basis for the coincidence of integrin activation and clustering
- the extracellular Ig domain of IAP(CD47), when bound to thrombospondin, interacts with integrin alphaIIbbeta3 and can change alphaIIbbeta3 in a high affinity state without the requirement of intracellular signaling
- signaling through JAM-1 and alphavbeta3 is necessary for bFGF-induced angiogenesis.
- Data provide the first evidence that alphavbeta3 integrin can generate apoptosis-stimulating signals.
- alphavbeta3 expression in LNCaP (beta3-LNCaP) prostate cancer cells causes increased cdc2 mRNA levels as evaluated by gene expression analysis, and increased cdc2 protein and kinase activity levels
- identification of binding site in gamma C-domain of fibrinogen
- alpha 5 beta 1 and alpha v beta 3 are both important but cell-specific fibrillin-1 receptors
- Low concentrations of agonists resulted in enhanced platelet aggregation in subjects with the GNB3 CC-genotype compared to carriers of a 825T-allele.
- relationships between the expression levels of CD61, CD63, and PAC-1 on the platelet surface and the incidences of acute rejection and tubular necrosis as well as the recovery of graft function after renal transplantation
- analyzed the possible cooperation effect between the PlA2 allele (GPIIIa) and LL genotype (SLC6A4) in the development of myocardial infarction
- high prevalence of the HPA-1b allele of beta3 in Glanzmann thrombasthenia
- integrin alphavbeta3 cooperates with metalloproteinase MMP-9 in regulating migration of metastatic breast cancer cells
- Individuals homozygous for the Leu33Pro polymorphism of the beta3 integrin subunit have an increased cancer risk.
- Glycoprotein IIb/IIIa has a role in platelet-activating factor-induced platelet activation with protein kinase C activity
- the 749EATSTFT756N and 755TNITYRG762T regions of beta3 contribute to the regulation of alphaIIbbeta3 anchorage to the cytoskeleton and platelet spreading to an adhesive surface
- beta3 integrin has a role in causing structural rearrangements of the 10-23 DNAzyme
- predictions of hydrophilicity, surface accessibility and antigenicity and the three dimensional structure of the beta(3) integrin correlate with autoantibody binding to a recombinant GPIIIa peptide 'B' containing residues 468-691.
- increased expression of the alpha(v)beta(3) integrin during breast cancer progression can make tumor cells more responsive to malignancy-promoting ligands such as OPN and result in increased tumor cell aggressiveness.
- the E749ATSTFTN756 region of the beta3-tail stabilizes the binding of soluble and surface-bound ligand to integrin alphaIIbbeta3 via a mechanism that involves the phosphorylation of FAK
- integrin alpha(v)beta3 has an important role in bone metabolism and angiogenesis, and in controlling growth of metastatic prostate cancer cells in the bone
- proteins regulated by CBFA2 are required for inside-out signal transduction-dependent activation of GPIIb-IIIa
- results invoke a model whereby Src is primed for activation by direct interaction with an integrin beta tail, and integrin clustering stabilizes activated Src by inducing intermolecular autophosphorylation
- The mutation at the position 29 107 of intron 10, G-->T, in the beta3 genomic DNA was found to induce an aberrant splicing and to be responsible for Glanzmann's thrombasthenia in this patient
- activation of the beta subunit I-like domain of integrin beta3 is analogous to that of the alpha subunit I domain
- Cysteine residues in the epidermal growth factor domains of the beta3 subunit are important for the ligand binding activity of the integrin alphaIIbbeta3 complex.
- CD47-alpha(v)beta(3) interactions that lead to integrin clustering are detergent resistant and do not require cholesterol or the transmembrane region of CD47
- alphavbeta3 integrin has a role in regulating proliferation and apoptosis of hepatic stellate cells
- in the absence of GPIbalpha or following inhibition of thrombin binding to GPIbalpha, there is a reduction in the thrombin-induced calcium flux, beta3 cleavage and micro -calpain activation.
- analysis of the three-dimensional model of integrin alphaIIbbeta3
- APOC3, LPL and GpIIIa genes were found to be associated with BP levels. The contributions of these genes, although modest, are consistent with the polygenic nature of blood pressure levels.
- integrin alpha(IIb)beta3 adhesive function is regulated by platelet FXIII and calpain
- This review discusses the major role played by glycoprotein IIb/IIIa (GPIIb/IIIa) in the regulation of platelet adhesion and aggregation during hemostasis.
- alpha IIb beta 3 signaling in platelets is regulated by SHIP1 and Lyn kinase
- GpIIIa gene polymorphism as a risk factor for stroke caused by large vessel disease in males.
- results indicate a new interaction partner for the alpha(v)beta(3) integrin, the TGFbetaIIR, in which TGFbeta1-induced responses are potentiated in the presence alpha(v)beta(3) ligands
- CD151 is essential for normal platelet function and that disruption of CD151 induced a moderate outside-in integrin alpha(IIb)beta(3) signaling defect
- For HPA-1 gene polymorphism, only the HPA-1a/a (PlA1/A1) genotype was observed in Korean population cardiac patients
- secondary structure analysis of integrin alphaVbeta3
- The HPA-1b (PlA2) allele significantly protected against subarachnoid hemorrhage.
- analysis of F11R dimerization, phosphorylation and complex formation with the integrin GPIIIa in human platelets
- Data suggest that modulating the expression of integrin subunits beta3/5 in human neurons may enhance adenoviral infectivity via the coxsackie-adenovirus receptor.
- beta3 integrins play important roles during transendothelial migration of PC3 cells while interacting with the matrix underneath the endothelium. These interactions are independent of the ability to cluster beta3 integrins into focal adhesions.
- CEACAM1 and alpha(v)beta(3) integrin are functionally interconnected with respect to the invasive growth of melanomas
- PlA1/A2 polymorphism of platelet glycoprotein IIIa (GPIIIa) has is not implicated in the pathogenesis of type 2 diabetes.
- A novel 465T-->C (S123P) mutation in exon 3 interfered with the platelet aggregation in a manner similar to Glanzmann's thrombasthenia.
- Inheritance of the integrin beta3 Leu33Pro polymorphism may increase the breast cancer risk by age 45 in the German population.
- a novel missense mutation of Ile304 to Asn in addition to the missense mutation His280 to Pro in the integrin beta3 gene as a cause of the absence of platelet alphaIIbbeta3 in Glanzmann's thrombasthenia
- The Integrin beta3 receptor is expressed on almost all cells originating from the mesenchyme and seem to mediate several biological processes, including adhesion of osteoblasts to the bone matrix.
- Mu-calpain is activated in human endometrial cells during hypoxia and that subsequent cleavage of the integrin beta3 cytoplasmic domain may give some adverse effects to the function of human endometrium
- Pyk2 is a phosphorylated beta(3) binding partner, providing a potential structural and signaling platform to achieve alpha(V)beta(3) -mediated remodeling of the actin cytoskele
- there is a phosphorylation-dependent association between beta(3) integrin and Vav1 which is essential for cell progression to a Rho-dominant phenotype during cell adhesion
- integrin alphaIIbbeta3 has a role in mechanotransduction for shear activation of platelets
- alphaIIb-mediated outside-in signaling resulting in TxA(2) production and granule secretion is negatively regulated by a sequence of residues in the membrane distal beta3 cytoplasmic domain sequence RKEFAKFEEER.
- association between early fetal loss and the presence of the PLA2 polymorphism of the ITGB3 gene
- Of the 22 Glanzmann thrombasthenia patients studied, mutations were detected in 12 individuals. Of these, 11 were novel mutations.
- We conclude that the ectodomain of alphaVbeta3 manifests a bent conformation that is capable of stably binding a physiological ligand in solution.
- This study provides evidence that ITGB3 plays a role in the pathogenesis of asthma and sensitization to mold allergens.
- dephosphorylation events play a role via serine/threonine phosphatases during the integrin outside-in signaling mechanism, and the Leu(33) --> Pro polymorphism regulates this process
- Epidermal growth factor receptor and alphavbeta3 work together as coreceptors for Human cytomegalovirus entry and signaling.
- variation in ITGB3 in addition to Leu33Pro could contribute to susceptibility to cardiovacular disease and serotonin in a sex-specific manner
- Results support the hypothesis that carriers of the GP IIIa PlA2 allele might have an increased risk for acute coronary syndrome in Hungarian population.
- SHPS-1 negatively regulates platelet function via CD47, especially alpha(IIb)beta(3)-mediated outside-in signaling
- TPO integrates G(i), but not G(q), stimulation, supports integrin alpha(IIb)beta(3) activation platelet aggregation independently of phospholipase C but requires PI3-kinase and Rap1B
- an apparently silent beta3 mutation results in aberrant splicing and reduced beta3 mRNA and may play a causative role in Type I Glanzmann thrombasthenia
- electron tomography analysis of the three-dimensional structure of integrin alphaIIbbeta3 in the active state
- PI3-K and calcium oscillation are synergistically operated and form a positive-feedback regulation in integrin alpha(IIb)beta3-mediated outside-in signaling
- Gal3ST-2 is involved in tumor metastasis process by regulation of adhesion ability to selectins and expression of integrin alphaV but not beta3
- reactive oxygen species produced in platelets significantly affected alphaIIbbeta3 integrin activation
- platelet membrane integrins alpha IIb(beta)3 (HPA-1b/Pl) and alpha2(beta)1 (alpha807TT) polymorphisms may have a role in premature myocardial infarction
- melanoma cells cross-present, in an alpha v beta3-dependent manner, an antigen derived from secreted matrix metalloproteinase-2 (MMP-2) to human leukocyte antigen A*0201-restricted T cells
- integrin alphavbeta6, alphavbeta3, alphavbeta5, alpha5beta1 and alpha9beta1 binding to osteopontin is controlled by specific structural motifs that are recognized by extracellular proteases
- Highest expression of alphavbeta3 integrin is associated with metastatic potential of osteosarcoma cells by enhancing the ability of the cells to migrate specifically to the lung
- the engagement of alphaIIb beta3 by the C-terminal sequence of the fibrinogen gamma-chain initiates signals that suppress subsequent fibronectin assembly by spread platelets
- GPVI and integrin alphaIIb beta3 have roles in signaling during platelet adhesion and platelet aggregation [review]
- Data show that alpha(v)beta3/Tat interaction triggers the activation of NF-kappaB in endothelial cells in a focal adhesion kinase-, RhoA- and pp60src-dependent manner, and this activation is required for motogenic activity of Tat in endothelial cells.
- ITGbeta3 subunit cytoplasmic domains require membrane anchorage and the NPXY motif to recruit to adhesion complexes
- Data show that protein-tyrosine phosphatase (PTP)-1B is an essential positive regulator of the initiation of outside-in alphaIIbbeta3 integrin signaling in platelets.
- Glycoprotein IIIa allele has been found in many studies to associate with risk of thrombosis.
- alphavbeta3 integrin is transiently up-regulated (and activated) in graft arteriopathy
- The 177-184 sequence of beta3 is necessary for vitronectin binding to alphaVbeta3.
- Cells overexpressing beta3 integrin can bind human ADAM12 via beta3 integrin.
- PI3K and granular released ADP in coordinating the feedback regulations in integrin alpha(IIb)beta3-mediated platelet activation
- beta3 knockout mice transfused with wild-type platelets generated specific anti-beta3 antibodies which were able to induce thrombocytopenia in wild-type mice.
- ITGB3 gene polymorphisms is associated with breast cancer
- Integrin beta(3) Leu33Pro homozygotes have an increased risk of ovarian cancer.
- analysis of platelet integrin alphaIIbbeta3 structure and function (review)
- the beta3 integrin gene is the direct target of NFAT1 in osteoclast formation
- Outside-in signaling through integrin alpha2beta1 triggered inside-out activation of integrin alphaIIbbeta3 and promoted fibrinogen binding. (alpha2beta1 AND integrin alphaIIbbeta3
- three beta-propeller mutations do not affect the production of pro-alpha(IIb), its ability to complex with beta3, or its stability, but do cause variable defects in transport of pro-alpha(IIb)beta3 complexes from the endoplasmic reticulum to the Golgi
- Our results did not show the causative relationship between the existence of platelets GP IIIa mutations and venous system thrombosis in the women in labor.
- Expression of integrins alphavbeta1, alphavbeta3, and alphavbeta5 in cerebral arteriovenous malformations and cavernous malformations. (Integrins alphavbeta1, alphavbeta3, and alphavbeta5)
- the signal interaction between IGF-1R and alpha(v)beta3 integrin plays an important role in promoting the development and progression of cervical cancer
- cardiotoxin A5 binds to alpha(v)beta3 integrin and inhibits bone resorption
- The endometrial expression of beta3 integrin subunit is reduced in the presence of tubal phimosis or hydrosalpinx, during the window of implantation.
- Inhibition of alphaIIbbeta3 activation by NAD(P)H oxidase inhibitors and superoxide scavengers was independent of NO/cGMP signaling demonstrating a direct role of platelet NAD(P)H oxidase-generated ROS for integrin alphaIIbbeta3 activation.
- T cells from kidney allograft recipients can express significant amounts of alpha-beta 3 integrin (CD41/CD61.
- These results establish the beta3 gene as a direct target of NFATc1 in RANKL-dependent osteoclast formation.
- the Pro33 isoform of purified integrin alphaIIbbeta3 has increased fibrinogen and prothrombin binding
- P-selectin expression, but not activated GPIIb/IIIa, is enhanced in ADP-activated platelets in the inflammatory stage of Takayasu's arteritis. SELP may play a significant role in the inflammatory and thrombotic responses associated with intractable TA
- Results describe the multifunctional behavior of integrins, including integrin beta 3, based on their modification potential at hydroxyl groups of amino acids as a source of interplay.
- while the C-terminal region of the alpha(IIb) tail minimally influences alpha(IIb)beta(3) activation, the C-terminal region of the beta(3) tail is critically involved
- Data showed that ITGB3 and SLC6A4 expression levels are correlated.
- analysis of metal-binding sites in the integrin beta3 A-domain
- we show that a coding variant of ITGB3 is associated with autism susceptibility in a large multiplex sample (P = 0.00082), and that this variation has different effects in males and females (P = 0.0018).
- integrin alpha3beta1 is a receptor for the alpha3NC1 domain and transdominantly inhibits integrin alphavbeta3 activation
- binding of CXCL12 to its receptor leads to enhanced expression of alpha5 and beta3 integrins
- binding of resveratrol to integrin alphaVbeta3, principally to the beta3 monomer, is essential for transduction of the stilbene signal into p53-dependent apoptosis of breast cancer cells.
- PET Molecular imaging of alpha(v)beta3 expression with [18F]Galacto-RGD in humans correlates with alpha(v)beta3 expression as determined by immunohistochemistry.
- Results suggest that shear stress directly modulates alpha(IIb)beta(3) function and that the shear-induced signaling contributes to regulation of platelet aggregation by directing the release of constraining cytoskeletal elements from the beta(3)-tail.
- beta3 integrin alters TGF-beta signaling in mammary epithelial cells via Src-mediated TbetaR-II tyrosine phosphorylation, which significantly enhanced the ability of TGF-beta to induce epithelial to mesenchymal transition and invasion.
- interactions between alphaIIbbeta3 & ligands echistatin & fibrinogen's gamma-module; studies differentiate priming ligands, which bind to resting receptor & perturb its conformation, from regulated ligands, where binding-site remodeling must first occur
- analysis of platelet integrin alphaIIbbeta3 mutations that may play a role in Glanzmann thrombasthenia
- Protein kinase C delta is positively regulated by platelet alpha IIb beta 3 outside-in signaling in platelet function.
- Tyrosine phosphorylation of beta3 promotes integrin outside-in signaling, by which the timing and location of calpain cleavage of integrins are dynamically regulated.
- N-cadherin and beta3 integrin expression correlates with progression to advanced-stage melanoma.
- platelet alphaIIbbeta3 is activated by an exogenous peptide corresponding to the transmembrane domain of alphaIIb
- The transition from a locally invasive phenotype to a metastatic phenotype may be primed by the elevated expression of alpha(v)beta(3) receptors in prostate cancer cells.
- Cell surface expression of alphavbeta5 resulted in an attenuation of alphavbeta3-mediated migration on vitronectin
- results demonstrate differential modes of regulation of Dok1 and Dok2 in platelets, and raise the possibility that Dok2 plays an important role in integrin outside-in signaling through a physical and functional interaction with integrin alphaIIbbeta3
- Transduction of beta3 integrin subunit cDNA confers on human keratinocytes the ability to adhere to denatured collagen (gelatin).
- identifies integrin alphaVbeta6 as a novel cellular receptor for fibrillin-1 with a K(d) of approximately 0.45 mum
- type I collagen induction of MT1-MMP-mediated MMP-2 activation is repressed by alphaVbeta3 integrin in human breast cancer cells
- beta3 integrin in MDA-MB-231 breast cancer cells may lead to increased MMP-2 expression and activity and might contribute to the enhanced invasive potential observed
- SLC6A4 and ITGB3 gene interactions have roles in autism etiology and in serotonin level determination
- The ITGB3_Leu33Pro polymorphism may potentially increase the risk of ovarian cancer in Polish women with an inherited BRCA1 mutation.
- Inhibition of integrin alphavbeta3 leads to abrogation of migration of HSC stimulated with PDGF-BB and to an antifibrogenic gene expression pattern.
- Individuals homozygous for the integrin beta3 Leu33Pro polymorphism have a two-fold risk of hip fracture, mainly confined to postmenopausal women.
- T cells epitopes on Glycoprotein IIIa.
- data suggest that the Pl(A2) polymorphism is a genetic determinant of ischemic stroke in a selected high-risk hypertensive population
- the surface density of fibrinogen affects alpha II b beta 3-mediated platelet signaling, adhesion, and spreading
- All patients with ISSNHL should undergo a comprehensive hematologic investigation of inherited and acquired prothrombotic factors, including platelet and V Leiden mutations, to identify a subset of patients at high risk of recurrent HL.
- in patients with stable coronary artery disease, an increased platelet reactivity after exercise, is specifically associated with an increased expression of platelet GP IIb/IIIa receptor
- No association between GpIIIa polymorphism with either type 2 diabetes or ST elevation acute myocardial infarction.
- Prostate cancer cells expressing fully functional but not dysregulated alphavbeta3 integrin are able to control their own adherence and migration to bone matrix, functions that facilitate tumor growth and control bone lesion development.
- This is the first demonstration that NO directly regulates integrin beta(3) phosphorylation.
- Discussion of recent developments in elucidating the mechanism of integrin activation, with particular focus on the platelet integrin alphaIIbbeta3, is provided in this review
- activation of ITGB3 transcription required a HoxA10 domain that was not identical to the "hexapeptide" that mediates interaction of Hox and Pbx proteins
- Necl-5 has a critical role in integrin alphavbeta3 clustering and focal complex formation
- integrins alphaLbeta2 and alphaIIbbeta3 are activated by mutation of a conserved asparagine in the I-like domain
- OPN plays a crucial role for tumor growth and angiogenesis of human lung cancer cells in vivo by interacting with alphavbeta3 integrin.
- Endothelial cell integrin alphaV beta3 is involved in sickle cell red blood cell adhesion.
- Data show that in bullous pemphigoid, beta3 integrins was detected mainly in basal keratinocytes and in inflammatory infiltrates in the dermis.
- LOX-1 is important for ADP-stimulated inside-out activation of platelet alpha(IIb)beta(3) and alpha(2)beta(1) integrins
- Results suggest that CTGF plays a crucial role in migratory/invasive processes in human breast cancer by a mechanism involving activation of the integrin-alphavbeta3-ERK1/2-S100A4 pathway.
- Significant associations between single nucleotide polymorphisms in ITGB3 and asthma, wheezing, and IgE levels suggest an early role for this gene in the development of asthma and allergy.
- Glycoprotein IIIa (GPIIIa, platelet antigen A1 and A2) polymorphisms that contribute to resistance or augmented response to aspirin are rare in the Chinese population.
- HPA-3b homozygotes have a tendency to develop ST segment elevation myocardial infarction instead of non-ST segment elevation infarction or unstable angina in acute coronary syndrome.
- Expression of the human ITGB3 subunit in mouse smooth muscle cells enhances IGF-I-stimulated signaling and cell proliferation.
- separation of transmembrane domains is required for integrin outside-in signal transduction
- establishes alphavbeta3 integrin as one key component of the transendothelial migration process of tumor cells, and as a potential target for anti-metastatic therapy
- Regions of alpha(IIb) and beta(3) cytoplasmic tails, together with membrane segments of the subunits, contact each other to form a complex which restrains the integrin in a resting state. It is unclasping of this complex that induces integrin activation.
- c-Src controls functional association between integrin alphav-beta3 and VEGFR-2 via integrin beta3 phosphorylation.
- The PIA2 allele did not alter the beneficial effect of statins on blood coagulation.
- Beta2-integrins and acquired glycoprotein IIb/IIIa (GPIIb/IIIa) receptors cooperate in NF-kappaB activation of human neutrophils
- preliminary findings show that the HPA-6 A/B genotype is a risk factor for autoimmune thrombocytopenia in systemic lupus erythematosus
- The proapoptotic ITGB3 cytoplasmic domain is rarely mutated in common human cancers and may not play an important role in the development of the cancers.
- Endometrial integrin beta3 and LIF expressions in the peri-implantation phase were significantly lower in stimulated cycles (including both moderate and high responders) compared to natural controls.
- Structural rearrangements undergone by integrin alpha(IIb)beta(3) after platelet activation were tested.
- effects of EDTA on GPIIb-IIIa dissociation and loss of adhesive functions are largely but not completely reve
- macrophage migration inhibitory factor directly up-regulates alphavbeta3 integrin and VEGF expression in human endometrial Ishikawa cells
- analysis of Src and C-terminal Src kinase interactions in integrin alphaIIbbeta3-mediated signaling to the cytoskeleton
- Our findings show that A2/A2 homozygosity is associated with an increased risk of visual loss due to anterior ischemic optic neuritis in giant cell arteritis.
- Truncated beta3 may act as an anti-integrin and play a crucial role in cell migration, which is an important process in tumor invasion and metastasis.
- platelet glycoprotein IIb-IIIa number (evaluated as maximal binding of (125)I-labelled antibody CRC64) varied from 40.5 to 80.8 x 10(3) per platelet with no significant influence of platelet glycoprotein III a(GP IIIa) genotype.
- Fourteen of the antibodies reacted with a 29-kDa GPIIIa fragment comprising only the GPIIIa hybrid and plextrin-semaphorin-integrin homology domains
- The specificity-determining loop was removed from the betaA domain & the mutations for HPA-4b,& -8,-6, -7, -10,-11,& -16bw antigens were introduced. The structural basis for their reactions with HPA-4 & HPA-bw antibodies was determined.
- oxidant systems external to platelets did not activate GP IIb/IIIa receptors while increased intra-platelet iron was associated with appearance of cytosolic oxidizing species and with GP IIb/IIIa receptor activation
- The precise nature of the integrin beta 3 domains involved in the HPA-1a epitopes was studied by creating 8 soluble peptides & modeling their structures & interations with HPA-1a antibodies. Residues outside the PSI and hybrid domains are required.
- The ITGB3 GCC-Pro33 haplotype was associated with enhanced vWF activity, which may indicate ITGB3 haplotype influences endothelial function.
- the studies demonstrate that Vbeta3 is a cellular receptor mediating both the cell adhesion and entry of KSHV into target cells through binding the virion-associated gB(RGD).
- a structural change in the conformation of anti-GPIIIa49-66 antibody contributes to its binding to the beta3 integrin and subsequent antibody-induced platelet fragmentation and aggregate dissolution
- Polymorphisms in human platelet alloantigen (HPA)-1 and HPA-3 (GPIIb/IIIa), HPA-2 (GPIb/IX), HPA-4 (GPIIIa) and HPA-5 (GPIa/IIa) were found to be associated with the symptoms and recurrence of ischemic stroke.
- Report observed that alphav-beta3 integrins and Focal Adhesion Protein-Tyrosine Kinases co-localize and that fibrillin filament attachment sites to endothelial cells merge with these molecules.
- Novel ancestral mutation found in a cluster of Jordanian Glanzmann thrombasthenia patients disrupts a conserved Cys549-Cys558 bond which results in reduced production of constitutively active alpha IIb beta 3.
- Interleukin-2, interleukin-12, and interferon-gamma levels and risk of young adult Hodgkin lymphoma
- uPAR is required to activate alphavbeta3 integrin in podocytes, promoting cell motility and activation of the small GTPases Cdc42 and Rac1
- platelet activation and aggregation is regulated by MMP-2 that specifically interacts with integrin alpha(IIb)beta(3)
- Upon phosphorylation of Tyr 747 in the beta3 integrin tail, however, Dok1 then binds much more strongly than talin.
- Real-time PCR showed that ETOH significantly altered the expression of genes involved in cell adhesion. There was an increase in the expression of alpha and beta Laminins 1, beta Integrins 3 and 5, Secreted phosphoprotein1 and Sarcoglycan epsilon.
- the beta3 membrane-proximal and -distal residues cooperatively regulate talin-mediated alpha IIb beta3 activation.
- Functional and computational studies of the ligand-associated metal binding site of ITGB3 are reported.
- Data show that integrin beta3 and VEGF expression can synergistically enhance tumor angiogenesis, and may play a crucial role in invasion and metastasis of gastric carcinoma.
- Tyr178 of beta3 is critical for alphaIIb maturation and macromolecular ligand binding to alphaIIbbeta3
- tyrosine phosphorylation of GIT1 by Src kinases may regulate cytoskeletal reorganization downstream of alpha(IIb)beta(3) by bringing the Rac guanine nucleotide exchange factor betaPIX to the vicinity of the integrin
- data show that the integrin beta 3 262T>C silent mutation is not restricted to a single family, as initially suggested
- Thermodynamic data demonstrate that entropy is the dominant factor stabilizing lphaIIbbeta3:echistatin binding, while transition-state thermodynamic parameters indicate that the rate of complex formation is enthalpy-limited.
- Integrin alpha v beta 3 plays a critical role in the S1P-stimulated chemotactic response of ECs.
- The shear-induced G(2)/M arrest and corresponding changes in G(2)/M regulatory protein expression and activity were mediated by alpha(v)beta(3) and beta(1) integrins through bone morphogenetic protein receptor type IA-specific Smad1 and Smad5.
- In conclusion, agglucetin, acting as a survival factor, promotes endothelial adhesion and angiogenesis by triggering alphavbeta3 signaling through FAK/phosphatidylinositol 3-kinase (PI3K)/Akt pathway.
- The numbers of antibody-binding sites of CD41, CD41a, CD41b, and CD61 on platelets of aplastic anemia patients and in idiopathic thrombocytopenic purpura patients, the numbers of sites for CD41 and CD41a were less than in normal controls.
- support an unsuspected role of alphaIIbbeta3/serotonin transporter(SERT)associations as well as alphaIIbbeta3 activation in control of SERT activity in vivo
- nature of the lipid headgroup affected only the intracellular part of the transmembrane helix, indicating that an asymmetric lipid distribution is not required for studying the beta3 transmembrane segment
- Mn(2+) simultaneously modulates the thiol isomerase activity of protein disulfide isomerase that is bound to alpha(V)beta(3) and induces its transition to the ligand-competent state, suggesting an alternative mechanism of integrin regulation
- RN181 associates with the platelet-specific integrin alphaIIbbeta3 in human platelets through the conserved alphaIIb cytoplasmic KVGFFKR motif.
- Protein phosphatase 2Ac (alpha) can negatively regulate integrin alpha(IIb)beta(3) signaling by suppressing the ERK1/2 signaling pathway
- Data show that in preeclamptic pregnancies, histamine does not stimulate expression of integrin alphaVbeta3, as it does in uncomplicated pergnancies.
- Infertile women with isolated polycystic ovaries are deficient in endometrial expression of osteopontin but not alphavbeta3 integrin during the implantation window.
- results demonstrate that TGFbeta1 induces alphavbeta3 integrin expression via a beta3 integrin-, c-Src-, and p38 MAPK-dependent pathway
- ROCK-dependent actomyosin-driven contractility is a critical determinant for the regulation of the interaction between Necl-5 and integrin alpha(V)beta(3).
- HCMV-induced angiogenic response depended on viral binding to and signaling through the beta(1) and beta(3) integrins and the EGFR, via their ability to activate the phosphatidylinositol 3-kinase and the MAPK signaling pathways
- Show that the Pl(A1/A2) polymorphism of Gp IIb/IIIa primarily affects the aggregation of platelets to monocytes in males.
- Data show no dramatic height change upon Mn(2+)-induced activation of membrane-bound integrins when compared with an inactive integrin control group.
- The results suggest that the intensity levels of platelet CD61 were elevated and associated with clinical phases and disease severity in patients with hemorrhagic fever with renal syndrome
- CRP at levels found during episodes of inflammation directly binds to the activated form of alphaIIbbeta3 and inhibits platelet aggregation.
- integrin alphavbeta3 binding to FGF1 plays a role in FGF1 signaling
- Results identify kindlin-2 as a novel regulator of integrin beta3 activation; it functions as a coactivator.
- ITGB3 (beta3) DNA from a Va(a)-positive individual were sequenced and a single-nucleotide polymorphism (C622>T) in exon 5, resulting in the replacement of threonine with methionine at residue 195 was identified.
- Osteoblast-derived BMP-2 act through Akt and ERK, which in turn activates IKK alpha/beta and NF-kappaB, resulting in the activations of beta1 and beta 3 integrins and contributing the migration of prostate cancer cells.
- vitronectin and integrin beta(3) contribute to the initiation of TLR2 responses
- osteopontin:alpha(v)beta(3)integrin complex untenable as marker of endometrial receptivity and implantation potential
- Talin recruitment to alphaIIbbeta3 by RIAM mediates agonist-induced alphaIIbbeta3 activation, with implications for hemostasis and thrombosis.
- talin IBS2 binds to the same face of the beta3 subunit cytoplasmic helix as the integrin alphaIIb cytoplasmic tail helix, suggesting that IBS2 can only interact with the beta3 subunit following integrin activation.
- integrins alphavbeta3 and alpha4beta1 may serve as receptors for sPLA2-IIA and mediate pro-inflammatory action of sPLA2-IIA
- Ligand binding to platelet alphaIIbbeta3 induces integrin cytoplasmic domain-dependent phosphorylation of FcgammaRIIa, which then enlists selected components of the immunoreceptor signaling cascade to transmit amplification signals into the cell.
- human umbilical vein endothelial cells adhere to immobilized CXCL4 through alphavbeta3 integrin, and also through other integrins, such as alphavbeta5 and alpha5beta1
- The presence of GPIIIa on this circulating trophoblast cellular material could be the source of HPA-1a alloantigen causing primary immunization of susceptible primigravidae
- the 4 biomarkers CLU, ITGB3, PRAME and CAPG may be used as prognostic factors for patients with stage III serous ovarian adenocarcinomas.
- The alphaIIb G236E mutation causes Glanzmann's thrombasthenia by impairing the association with beta3 during biogenesis of the receptor.
- UV-C appears to activate alphaIIbbeta3 not by affecting intracellular signal transduction, but by reduction of disulfide bonds regulating integrin conformation.
- SDF-1alpha enhances the invasiveness of chondrosarcoma cells by increasing alphavbeta3 integrin expression through the CXCR4/ERK/NF-kappaB signal transduction pathway.
- The apo(a) component of Lp(a) signals through integrin alphaVbeta3 to activate endothelial cells.
- alpha3 integrin, despite its ability to respond to alternate ligands after wounding, does so through a single structure, the filopodia.
- This report analyzed the molecular effects of an A>T substitution leading to an amino acid change, D217>V, in the beta3 integrin gene identified in patients with variant Glanzmann Thrombasthenia.
- Mutation of the clasp region of alphav or beta3 results in a constitutively activated integrin, confirming the role of the extracellular clasp in restraining integrin activation.
- These results demonstrate that integrin alphavbeta3-mediated transcriptional regulation of MMP-9 and TIMP-1 is critical for suppressing the ovarian cancer cell invasion.
- VWF strings bind specifically to integrin alpha(v)beta(3) on human endothelial cells
- alpha(IIb)beta(3) provides a constitutive presence of factor H on platelets; activation of platelets increases platelet-bound factor which perhaps involves other binding sites for factor H on platelets.
- Glutathione regulates integrin alpha(IIb)beta(3)-mediated cell adhesion under flow conditions.
- The genetic polymorphism glycoprotein IIIa leu33pro was significantly associated with CHD.
- Analogous to EAE, in subjects with MS, we found increased expression of Opn in DCs and increased expression of the Opn receptors CD44, beta(3), and alpha(v) on T cells.
- analysis of how the different antiangiogenic potential of resveratrol stereoisomers is related to their different capacity to affect alphavbeta3 integrin function
- Ligation of alpha(IIb)beta(3) by immobilized ligands during platelet adhesion induces a transmembrane conformation change in the integrin, resulting in unclasping of the complex between the membrane-proximal parts of cytoplasmic tails.
- pneumococci exploit the vitronectin-alphavbeta3-integrin complex as a cellular receptor for invasion and this integrin-mediated internalization requires the cooperation between the host signalling molecules ILK, PI3K and Akt.
- Beta3 integrin expression within uterine endometrium may have a role in unexplained infertility
- Data show that cilengitide activated cell surface alphaVbeta3, stimulated phosphorylation of FAK, Src and VE-cadherin, increased cell permeability and decreased cell adhesion in endothelial cells.
- These results confirm the impact of polymorphism A2 (PLA2) in platelet glycoprotein IIb/IIIa on recurrent spontaneous abortion in the period of 10 to 20 weeks of gestation.
- Glycoprotein IIB/IIIA inhibitor to reduce postpercutaneous coronary intervention myonecrosis and improve coronary flow in diabetics: the 'OPTIMIZE-IT' pilot randomized study.