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Validated All-in-One™ qPCR Primer for ITGA4(NM_000885.5) Search again
Product ID:
HQP009798
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD49D, IA4
Gene Description:
integrin subunit alpha 4
Target Gene Accession:
NM_000885.5(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain. [provided by RefSeq].
Gene References into function
- Alpha 4 integrin signaling activates phosphatidylinositol 3-kinase, stimulates beta 2 integrin-mediated T cell adhesion to ICAM-1, and induces a distinct rearrangement of the actin cytoskeleton.
- role in mediating adhesion and migration of activated cycling CD34+ hematopoietic progenitor cells onto fibronectin
- Human mast cell progenitors use alpha4-integrin, VCAM-1, and PSGL-1, E-selectin for adhesive interactions with human vascular endothelium under flow conditions.
- important for migration of chronic lymphocytic leukemia cells into lymph nodes
- Adhesion to fibronectin via alpha4 integrin (CD49d) protects B cells from apoptosis induced by serum deprivation
- mediates adhesion of osteosarcoma to the core region of thrombospondin 1 (alpha 4 beta 1 integrin)
- VCAM-1/alpha4 integrin (CD49D antigen) interactions mediate monocyte adhesion to human saphenous vein
- VLA4 integrin activation by chemokines requires cholesterol
- role of Rap1 GTPase for Mn(2+)- and antibody-induced VLA-4-mediated cell adhesion [VLA-4]
- regulation of alpha4beta1 integrin function in melanoma cells and T cells by ligands of CD47
- cell surface alpha4 integrin is downregulated by p38 MAP kinase to facilitate erbB-2-mediated invasion
- 3 new "silent' ITGA4 variants: a C-->A transversion at 269 in the promoter region of exon 1; a G-->A transversion at 2273 in exon 16, & a T-->C exchange at 3311 in exon 26 are described. The frequencies of all 5 known variants were measured.
- endometrial integrin alpha1 and alpha4 expression is more consistently present in the early luteal phase in stimulated cycles than in natural cycles
- Association of integrin alpha 4 with paxillin mediates cross-talk by enhancing the activation of the related tyrosine kinases FAK and Pyk2 in Jurkat T cells.
- Requires integrin alpha4beta1 to control T cell migration and requires both phosphorylation and dephosphorylation of the alpha 4 cytoplasmic domain to regulate the reversible binding of paxillin.
- Alpha4beta1 integrin has a role in cell adhesion
- in beta-thalassemic patients, significant reduction of CD49d, CD29 and CD71 antigen expression was found in peripheral blood nucleated red cells
- following priming of human neutrophils with lipopolysaccharide or tumor necrosis factor alpha (TNF-alpha), addition of formyl-Met-Leu-Phe (fMLP) results in a "stimulated", sepsis-like, four- to fivefold rise in CD49d expression
- integrin alpha4beta1 is induced by p53 and has a role in B-cell chronic lymphocytic leukemia drug resistance
- cellular alpha4-integrin is conformationally activated
- integrin alpha-4 has a role in redox regulation of surface protein thiols
- alpha3beta1, alpha4beta1 and alphaVbeta1 integrins may play an important role in the implantation process
- Alpha4beta1 is an important endothelial cell receptor for mediating motility and proliferative responses to thrombospondins and for modulation of angiogenesis.
- Role of alpha4, alpha5, and alphav integrin receptors--which are central to mediating interactions with these domains of FN--in regulating ssquamous cell carcinoma migration.
- EWI-2-dependent reorganization of alpha4beta1-CD81 complexes on the cell surface is responsible for EWI-2 effects on integrin-dependent morphology and motility functions
- findings identify Glu-Pg as an adhesive ligand for integrins alphaMbeta2 and alpha5beta1 and suggest that alpha5beta1 may participate in the binding of soluble Glu-Pg and assist in its activation.
- conformational activation of VLA-4 by inside-out signaling is independent of and additive to reduction-regulated integrin activation
- very late antigen-4 affinity is modulated by shear
- regulatory role of alpha 4 integrins on T lymphocyte-antigen presenting cell cognate immune interactions
- alpha4beta1 integrin on sickle reticulocytes is a CD47-activated receptor for TSP, VCAM-1, and plasma fibronectin
- Cell adhesion to VCAM-1 mediated by very late antigen 4 was suppressed by monoclonal antibodies, dependent on reaction oxygen species.
- metal ion binding sites in the I-like domain and the interface with the hybrid domain are important for rolling and firm adhesion by integrin alpha4beta7
- demonstrates a cooperative role between alpha5beta1 and alpha4beta1 integrins and suggests that interactions between the Hep II domain and alpha4beta1 integrin could modulate the strength of cytoskeleton-mediated processes in the trabecular meshwork
- VEGF and alpha4beta1 integrin have roles in chemokine-induced motility on and through endothelium in chronic lymphocytic leukemia cells but not in normal B lymphocytes
- analyzed the rotavirus-specific VH and VL repertoire in IgD- B cells expressing the intestinal homing marker alpha4beta7
- activation of Vav1-Rac signaling pathway by CXCL12 represents an important inside-out event controlling efficient up-regulation of alpha4beta1-dependent T lymphocyte adhesion
- ITGA4 mediates engraftment of GCSF-mobilized stem cells.
- CD44 and CD49d are putative activity markers and CD44 a potential novel therapeutic target in multiple sclerosis.
- These data suggest that during a respiratory virus infection activated CD8+ T cells from asthmatic subjects may produce excess type 2 cytokines and may contribute to asthma exacerbation by augmenting allergic inflammation.
- Distinct structural requirements for the VLA-4 integrin alpha 4 subunit reflect different roles played by VLA-4 under static adhesion and shear flow conditions.
- VLA-4 modulation by PLZF may represent an important step in the control of normal and leukemic cell mobilization
- There is a novel alpha4 cytoplasmic domain connection leading to c-Src activation which functions as a FAK-independent linkage to a common motility-promoting signaling pathway.
- We demonstrate that cell movement in response to IL-8 is mediated by Mac-1, whereas LFA-1 is required for directional migration. By contrast, chemotaxis to fMLP requires Mac-1 for cell movement.
- This is the first demonstration that cytoskeletal anchorage of an integrin enhances the mechanical stability of its adhesive bonds under strain and, thereby, promotes its ability to mediate leukocyte adhesion under physiological shear stress conditions.
- Alpha4beta1 (VLA-4) integrin is a major galectin-1-glycosylated counterreceptor involved in immune developmental synapse formation.
- transcriptional activation of the alpha4integrin gene by Wt1(-KTS) might contribute to normal formation of the epicardium and other tissues in the developing embryo
- comparison of DOCK2 and Vav1 involvement in CXCL12-promoted Rac activation and alpha4beta1-dependent human T cell adhesion indicated a more prominent role of Vav1 than DOCK2
- Review describes the role of alpha 4-integrin in the pathophysiology of demyelinating diseases and the findings which led to the targeting of alpha 4-integrin for the treatment of multiple sclerosis.
- CD14 is a novel ligand for alpha4beta1, exhibiting similar activation-state dependent binding characteristics as other alpha4beta1 ligands.
- High affinity interactions between the integrin alpha4beta1 on melanoma cells and VCAM-1 on activated endothelial cells may enhance the metastatic capacity of human beta2/beta3-negative melanoma cells.
- This study identified the beta1 integrins on primary smooth muscle cells that fibulin-5 interacts with, and showed that failure of fibulin-5 to activate these receptors limits cell spreading, migration and proliferation.
- The VCAM-1/very late activation antigen 4 adhesive system critically mediates endothelial progenitor cell adhesion to cultured rheumatoid arthritis fibroblasts and to rheumatoid arthritis synovial tissue.
- talin 1 is a versatile VLA-4 affinity regulator implicated in both spontaneous and chemokine-triggered rapid adhesions to VCAM-1.
- The data of thie study only modestly argue for a role of ITGA4 in determining susceptibility to MS.
- Relevance of CD49d protein expression as overall survival and progressive disease prognosticator in chronic lymphocytic leukemia.
- G-CSF treatment induced reduced expression of CXCR4, VLA-4 and VEGFR-1 in patients with liver failure.
- Data show that NG2 and integrin alpha4 oppositely regulate anoikis in fibroblasts, and that NG2 and integrin alpha4 regulate FAK phosphorylation by PKCalpha-dependent and -independent pathways, respectively.
- B-CLL cells attached to and bound proMMP-9 and active MMP-9, and this was inhibited by blocking the expression or function of alpha4beta1 or CD44.
- Data suggest that cooperation between alpha(4)beta(1)/alpha(5)beta(1) and RGD integrins is important for epicardial mesothelial cell adhesion and subepicardial matrix formation.
- activation of functional alpha(4) integrin activity in resting eosinophils is mediated by Rap1 provided that the intracellular-free Ca(2+) is at a normal homeostatic concentration.
- the CXCR4/SDF-1 axis interacts with VLA-4 in regulating migration and adhesion of Waldenstrom macroglobulinemia cells in the bone marrow microenvironment
- we describe the mechanism of activation of the alpha4beta1 integrin on sickle reticulocytes and discuss novel roles for this integrin in SCD as a result of this activation[review]
- VLA-4, CX3CR1, and CXCR4, involved in T-cell homing, had increased expression in ITP patients
- study reports that T cell costimulation by the integrin VLA-4 (alpha4beta1) required SLP-76 domains implicated in microcluster assembly
- LPS or peritoneal dialysis effluent stimulated the adherence of T cells to mesothelial cells, a process mediated by the integrins alpha6beta1 and alpha4beta1
- In CD56(bright) cells from fertile but not infertile women, alpha(4) integrin increased between day 5 and the preovulatory luteinizing hormone surge.
- Integrin VLA-4 enhances sialyl-Lewisx/a-negative melanoma adhesion to and extravasation through the endothelium under low flow conditions.
- integrins alphavbeta3 and alpha4beta1 may serve as receptors for sPLA2-IIA and mediate pro-inflammatory action of sPLA2-IIA
- indicate CD49d >or=30% as a new marker for the initial prognostic assessment of Binet A chronic lymphocytic leukemia
- hypermethylation of CDH1 and integrin alpha4 genes may be used as recurrence-associated prognostic indicators in stage I and stage II esophageal squamous cell carcinoma, respectively.
- CD49d provides access to highly pure populations of untouched Foxp3(+) Treg cells conferring maximal safety for future clinical applications
- Integrin activation is required for the dissociation of JAM-L-VLA-4 complexes and the accumulation of functional JAM-L dimers, which indicates that the leukocyte integrin VLA-4 controls JAM-L function in cis by controlling its dimerization state.