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Validated All-in-One™ qPCR Primer for ITGA2(NM_002203.3) Search again
Product ID:
HQP009794
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
BR, CD49B, GPIa, HPA-5, VLA-2, VLAA2
Gene Description:
integrin subunit alpha 2
Target Gene Accession:
NM_002203.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene product belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane glycoproteins composed of a distinct alpha chain and a common beta chain. They are found on a wide variety of cell types including, T cells, fibroblasts and platelets. Integrins are involved in cell adhesion and also participate in cell-surface mediated signalling. [provided by RefSeq].
Gene References into function
- Bromodeoxyuridine induces integrin expression at transcriptional (alpha2 subunit) and post-transcriptional (beta1 subunit) levels, and alters the adhesive properties of two human lung tumour cell lines.
- Reduction in integrin alpha 2 is related to changes seen during immortalization and malignant progression
- Trimucytin, a collagen-like snake venom protein, activates platelets independent of I-domain within alpha2 subunit of alpha2beta1 integrin.
- the rGPIa/IIa-collagen interaction dominates the adhesion of rGPIa/IIa-Ib alpha-liposomes to the collagen surface at low shear rates; the rGPIa/IIa-collagen and rGPIb alpha-VWF interactions synergistically support liposome adhesion at high shear rates.
- Data report the identification of signaling pathways required for suppression of integrin alpha2beta1 function by c-erbB2.
- initial adhesion of endometrial cells to mesothelium is not mediated by alpha(2)beta(1)integrin
- findings demonstrate that E-cadherin can interact with alpha2beta1 and suggest that heterotypic interactions between E-cadherin and integrins may be more common than originally thought
- role of the GP Ia C807T/G873A polymorphism as a risk factor for thrombosis in Behcet disease
- R-Ras promotes focal adhesion formation by signaling to FAK and p130(Cas) through a novel mechanism that differs from but synergizes with the alpha2beta1 integrin.
- genetic variation of platelet glycoprotein Ia may play a particularly important role during the advanced stages of diabetic retinopathy
- twins developed ischaemic strokes and were shown to be homozygous for the alpha2 807T allele
- linkage with extracellular signal-regulated kinase is functionally linked in highly malignant autocrine transforming growth factor-alpha-driven colon cancer cells
- Data show that NADPH oxidase activation production of reactive oxygen species are involved in the increase of alpha2beta1-integrin plasma membrane expression in Caco-2 cells stimulated with type IV collagen.
- role of polymorphisms in cardiovascular thrombotic disease [review]
- signaling through both constitutively expressed alpha2 integrin and Matrigel-induced alpha3 integrin expression is required to acquire a differentiated phenotype in Caco-2 cells.
- capillary morphogenesis requires endothelial alpha2beta1 integrin engagement of a single type I collagen integrin-binding site, possibly signaling via p38 MAPK and focal adhesion disassembly/FAK inactivation.
- adhesion to the alpha2beta1-specific peptide was disulfide-exchange dependent and protein disulfide isomerase (PDI) mediated in platelets
- results of a case-control study involving 180 stroke patients and 172 controls do not support a role for the integrin alpha2 C807T and GPVI Q317L polymorphisms in the development of first-ever ischemic stroke
- Monkey rotavirus binding to alpha2beta1 integrin requires the alpha2 I domain and is facilitated by the homologous beta1 subunit.
- role in inhibiting Fas-mediated apoptosis in T-lymphocytes
- There were significant differences in the distribution of T and C alleles between myocardial infarction and control groups.
- human platelet deposition on collagen depends on the concerted interplay of several receptors: GPIb in synergy with alpha(2)beta(1) mediating primary adhesion, reinforced by activation through GPVI, which further regulates the thrombus formation.
- results suggest that matrix metalloproteinase-1 can stimulate dephosphorylation of Akt protein and neuronal death through a non-proteolytic mechanism that involves changes in integrin alpha2beta1 signaling
- Since the platelet alpha2 C807T gene polymorphism is associated with alpha2beta1 receptor density on the platelet surface, the level of alpha2beta1 on platelets may be an additional factor affecting Glanzmann's thromboasthenia clinical expression.
- virus attachment to alpha(2) integrin on the cell surface was found to result in integrin clustering, which can give rise to signaling and facilitate the initiation of the viral entry
- VEGF-A induced alpha1 & alpha2 integrins, promoting lymphatic endothelial tube formation & haptotactic migration. Lineage-specific integrin receptor expression contribute to the distinct dynamics of wound-associated angiogenesis & lymphangiogenesis.
- alpha2beta1 integrin has a role in endorepellin-induced endothelial cell disassembly of actin cytoskeleton and focal adhesions
- alpha2beta1 integrin and GPVI regulate stress fiber formation in megakarocytes, the primary actin structures needed for cell contraction
- alpha2beta1 integrin and CD44/CSPG receptor binding on human melanoma cell activation has been evaluated herein using triple-helical peptide ligands incorporating collagen peptides.
- ILK regulates alpha 2 beta 1 in HEL cells, is activated in platelets and associates with beta 1-integrins
- the interaction of platelet GP Ib with VWF mediates the activation of alpha2beta1, increasing its affinity for collagen
- Data demonstrate that type I collagen synergistically enhances platelet-derived growth factor (PDGF)-induced smooth muscle cell proliferation through Src-dependent crosstalk between the alpha2beta1 integrin and the PDGF receptor beta.
- alpha2807TT genotype of the platelet membrane integrin alpha2beta1 is associated with premature onset of early fetal loss. It appears that this risk factor does not induce the pathomechanism, but modulates the course of fetal loss
- Data show that the recombinant Scl protein p176 promotes adhesion and spreading of human lung fibroblast cells through an alpha2beta1 integrin-mediated interaction.
- Alpha2beta1 integrin is required for up-regulation of IL-1 beta-dependent airway smooth muscle secretory responses by fibronectin; while alpha2beta1 is also an important transducer for type I collagen.
- Binds to vimentin, but this association is lost after prolonged adhesion of endothelial cells to collagen.
- individual effect of each polymorphism located either in the coding or promoter sequence of the alpha2 gene may act in combination to modulate variations in platelets alpha2beta1 receptor density
- analysis of platelet glycoprotein I(b)alpha and integrin alpha2beta1 polymorphisms in diverse populations
- platelet membrane integrins alpha IIb(beta)3 (HPA-1b/Pl) and alpha2(beta)1 (alpha807TT) polymorphisms may have a role in premature myocardial infarction
- analysis of binding between collagen type III and integrins alpha1beta1 and alpha2beta1
- the signaling network involving Smad-dependent TGFbeta, PKCdelta, and integrin alpha2beta1/alpha3beta1, regulates cell spreading, motility, and invasion of the SNU16mAd gastric carcinoma cell variant
- alpha2beta1 and signaling via autocrine mediators facilitate and amplify the GPVI procoagulant activity of fibrillar and non-fibrillar collagens
- Integrin alpha-2 gene polymorphisms is associated with breast cancer
- Outside-in signaling through integrin alpha2beta1 triggered inside-out activation of integrin alphaIIbbeta3 and promoted fibrinogen binding. (alpha2beta1 AND integrin alphaIIbbeta3
- Our results did not show the causative relationship between the existence of platelets GP Ia mutations and venous system thrombosis in the women in labor.
- Results suggest that loss of E-cadherin function is linked to regulation of cell-cell and cell-matrix adhesion, based in part on cell surface expression of alpha2, alpha3 and beta1 integrins.
- The Platelet surface integrin, alpha 2-collagen interaction is an early step associated with platelet adhesion and activation and plays an important role in arterial thrombosis.
- Common polymorphism (C807/T807), affecting the GPIa gene expression, in the development of oral cancer.
- malignant phenotype of pancreatic cancer on type I collagen is mediated specifically by the alpha2beta1 integrin
- the Pro33 isoform of purified integrin alphaIIbbeta3 has increased fibrinogen and prothrombin binding
- Genetic polymorphism C807T of platelet glycoprotein Ia increases the risk for premature myocardial infarction. It is also an independent predictor for the release of sCD40L during acute MI and persists 1 year after event.
- Acquisition of multiple antitumor drugs was accompanied by a drastically reduced expression of alpha2beta1 in the adenocarcinoma cells.
- This meta-analysis does not support an association between the C807T polymorphism of ITAG2 gene and stroke.
- The ectopic expression of TM4SF5 in Cos7 cells reduced integrin signaling under serum-containing conditions, but increased integrin signaling upon serum-free replating on substrates.
- extracellular matrix fragments produced by apoptotic EC initiate a state of resistance to apoptosis in fibroblasts via an alpha2beta1 integrin/SFK (Src and Fyn)/phosphatidylinositol 3-kinase (PI3K)-dependent pathway
- Cell attachment was significantly inhibited by function-blocking anti-alpha2 (56%) and -beta1 (98%) integrin antibodies.
- Results show that alpha2beta1 integrin signalling enhances cyclooxygenase-2 expression in intestinal epithelial cells.
- Our findings support the view that C807T polymorphism of the GPla gene is not a significant risk factor for CAD, either alone or in combination with other major cardiovascular risk factors.
- results demonstrate differential modes of regulation of Dok1 and Dok2 in platelets, and raise the possibility that Dok2 plays an important role in integrin outside-in signaling through a physical and functional interaction with integrin alphaIIbbeta3
- C807T/G873A polymorphisms, but not T837C, are associated with higher platelet reactivity and residual platelet activity after treatment with anti-platelet agents
- glycoprotein Ia 807C/T and 873G/A dimorphisms were not shown as risk factors for VTE
- activated EGF receptor transiently modulates integrin alpha2 cell surface expression and stimulates integrin alpha2 trafficking via caveolae/raft-mediated endocytosis
- pancretic cancer cells attach to 3D type I collagen scaffolds in an alpha2beta1-specific manner
- Adhesion of breast cancer cells through the VLA integrins alpha2beta1 and alpha5beta1 was significantly reduced by an apoptosis-inducing natural triterpenoid, dehydrothyrsiferol (DT), when studied on low amounts of extracellular matrix.
- both the gpIb-VWF interaction and the integrin alpha(2)beta(1)-collagen interaction contribute to platelet adhesion under high shear stress; integrin alpha(II)beta(1) makes a greater contribution to adhesion to type I collagen because less VWF is bound
- study indicates that the GPIa 807 C/T polymorphism does not represent a risk factor for Buerger's disease itself, but could be associated with premature onset of this disorder in predisposed individuals
- findings suggest an important role of integrin alpha2beta1, alpha3beta1, and alpha5beta1 in the architectural characteristics of ameloblastomas and adenomatoid odentogenic tumor
- genetic variability within ITGA2 may confer risk for ischemic stroke independent of conventional risk factors.
- LOX-1 is important for ADP-stimulated inside-out activation of platelet alpha(IIb)beta(3) and alpha(2)beta(1) integrins
- There is no indication that the presence of 807C/T polymorphism is present in platelet glycoprotein Ia gene and a risk factor for retinal vein occlusion.
- CD44+alpha2beta1+ cell population is enriched in tumor-initiatin prostate cancer cells
- thrombopoietin-induced in vitro differentiation of primary human cord blood mononuclear cells into megakaryocytes, we observed rapid, progressive CpG methylation of ITGA1, but not PELO or ITGA2.
- analysis of Src and C-terminal Src kinase interactions in integrin alphaIIbbeta3-mediated signaling to the cytoskeleton
- Our results do not support an independent association between the 807C/T polymorphism and stroke of undetermined etiology.
- The result suggested that cytoskeleton was a possible mechanical sensor to centrifugal stimuli, and the cytoskeleton regulation to centrifugal loading was in an extracellular matrix-dependent and integrin-mediated manner.
- Polymorphisms in human platelet alloantigen (HPA)-1 and HPA-3 (GPIIb/IIIa), HPA-2 (GPIb/IX), HPA-4 (GPIIIa) and HPA-5 (GPIa/IIa) were found to be associated with the symptoms and recurrence of ischemic stroke.
- Initiation of the signal requires two collagen receptors, alpha2beta1 integrin and discoidin domain receptor (DDR). Each receptor propagates signals through separate pathways that converge to up-regulate N-cadherin.
- induction of decorin expression in angiogenic, as opposed to quiescent, endothelial cells promotes a motile phenotype in an interstitial collagen I-rich environment by both signaling through IGF-IR and influencing alpha2beta1 integrin activity
- collagen and a jararhagin-derived disintegrin peptide competitively bind to the integrin alpha2-I domain
- alpha2beta1 integrin clustering defines its own entry pathway that is Pak1 dependent but clathrin and caveolin independent and that is able to sort cargo to caveosomes
- The data suggest that phosphoenolpyruvate -dependent decrease of collagen biosynthesis in cultured human skin fibroblasts may undergo through depression of alpha(2)beta(1) integrin and IGF-IR signaling.
- Integrin alpha2beta1 may promote migration and invasion of neuroblastoma cells.
- These results indicate that NSP4 interaction with integrin alpha1 and alpha2 is an important component of enterotoxin function and rotavirus pathogenesis, further distinguishing this viral virulence factor from other microbial enterotoxins.
- 807C/T polymorphism of glycoprotein Ia (GPIa) gene were associated with higher risk of ischaemic stroke in atrial fibrillation
- Generated high throughput platform to examine interactions of type I collagen receptor alpha(2)beta(1) and fibronectin receptor alpha(5)beta(1) with peptide ligands to evaluate the effects of integrin cross-talk on adhesive responses.
- analysis of distinct roles of beta1 metal ion-dependent adhesion site (MIDAS), adjacent to MIDAS (ADMIDAS), and ligand-associated metal-binding site (LIMBS) cation-binding sites in ligand recognition by integrin alpha2beta1
- high-affinity binding for alpha2beta1 can control the overall platelet-adhesive activity of native collagens.
- NO targets activation-dependent adhesion mediated by alpha(2)beta(1), possibly by reducing bioavailability of platelet-derived ADP, but has no effect on activation-independent adhesion mediated by GPVI
- analysis of a prokaryotic Scl1 collagen sequence motif that mediates binding to human collagen receptors, integrins alpha2beta1 and alpha11beta1