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Validated All-in-One™ qPCR Primer for IRF1(NM_002198.2) Search again
Product ID:
HQP009778
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
IRF-1, MAR
Gene Description:
interferon regulatory factor 1
Target Gene Accession:
NM_002198.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion. [provided by RefSeq].
Gene References into function
- IRF-1 exerts a pivotal role in granulocytic differentiation, and its induction by G-CSF represents a limiting step in the early events of myeloid cell differentiation.
- Review: Stimulation of interferon regulatory factor-1 by prolactin
- IFNtau effect on IRF-1 expression is primarily regulated by tyrosine-phosphorylated Stat1alpha or Stat1beta dimers
- This review focuses on the induction and function of IRF-1 during M. tuberculosis infection
- This review focuses on IRF-1 is a negative regulator of cell proliferation.
- Isolation and characterization of a human STAT1 gene regulatory element. Inducibility by interferon (IFN) types I and II and role of IFN regulatory factor-1.
- Host defense responses to infection by Mycobacterium tuberculosis. Induction of IRF-1 and a serine protease inhibitor
- IFN regulatory factor-1 regulates IFN-gamma-dependent cathepsin S expression
- IRF-1 associated with resistance to antiestrogens
- Interferons inhibit tumor necrosis factor-alpha-mediated matrix metalloproteinase-9 activation via interferon regulatory factor-1 binding competition with NF-kappa B
- VCAM-1 expression via effects on interferon regulatory factor-1 expression and activity
- Promoter single nucleotide polymorphisms were detected and their possible contribution to T-lymphocyte helper 1 response in chronic hepatitis C was studied. Interferon-beta therapy increased the CD4(+) cell population.
- STAT-1, IRF-1, and RAR-beta expression were enhanced by IFN-gamma and ATRA in combination, and to a greater degree in BALM-3 cells than in BALM-1 cells, suggesting that these IFN-gamma related genes were involved in the induction of apoptosis.
- IRF-1 is controlled by two distinct signalling pathways; a JAK/STAT-signalling pathway in viral infected cells and an ATM-signalling pathway in DNA damaged cells.
- GRIM-19 protein inhibits the cell-transforming property of this protein via a physical interaction.
- IRF-1 and IRF-2 induced by IFN-gamma bind to three distinct IL-4 promoter sites and function as transcriptional repressors
- IRF-1-CEBPbeta complex activate the promoter of IL-18 binding protein.
- IRF-1 is a potential mediator of IFN-gamma-induced attenuation of telomerase activity and hTERT expression
- expression of IRF-1-dependent genes in neurons plays a role in ischemic neuronal death.
- Identification of the domain of interferon regulatory factor-1 responsible for transactivation.
- IRF1 represses CDK2 gene expression by interfering with SP1-dependent transcriptional activation.
- IRF1 is one of the transcription factors responsible for the induced coexpression of NF-kappa B activator 1 (Act1) and CD40 in lung epithelial A549 cells by inflammatory cytokines.
- These data indicate that interferon regulatory factor-1 is a hallmark of the gliadin-mediated inflammation in celiac disease.
- Expression of IRF-1 is altered in human endometrioid adenocarcinoma compared with normal endometrium and postmenopausal endometrium.
- the IRF1 HinfI gene polymorphisms do not appear to be involved in susceptibility to celiac disease
- IRF1 has a role in up-regulation of human caspase-8 by interferon-gamma in breast tumor cells
- Measles virus upregulates IRF-1 in a manner that is independent of IFN but dependent on the JAK/STAT pathway. This induction of IRF-1 appears to suppress cell growth, although the extent seems to vary among MeV strains.
- IRF-1 mediates the proapoptotic but not cell cycle arrest effects of the steroidal antiestrogen ICI 182,780
- Results suggest that the functional interplay between interferon regulatory factors 1 and 2 serves as an elaborate and cooperative mechanism for regulation of interleukin-7 production essential for local immune regulation within human intestinal mucosa.
- a novel tumor necrosis factor (ligand) superfamily, member 10-mediated tumor suppressor activity of interferon regulatory factor 1 and suggest a mechanistic basis for the synergistic antitumor activities of certain retinoids and interferons
- IFN regulatory factor 1 is involved in TNF-alpha-induced VCAM-1 expression and monocyte adhesion to TNF-alpha-activated endothelial cells.
- IRF-1 is one of the key host factors that regulate intracellular HCV replication through modulation of interferon-stimulated-gene-mediated antiviral responses.
- interleukin-12 p35 gene transcription is activated by interferon regulatory factor-1 and interferon consensus sequence-binding protein
- IRF-1-p300 interface as an allosteric modifier of DNA-dependent acetylation of p53 at the p21 promoter
- IFN-gamma enhances TRAIL-induced apoptosis through IRF-1
- therapies designed to enhance IRF-1 expression within tumor cells may represent novel treatment strategies for breast cancer
- IFN-gamma-IRF system is involved in BPAG1 gene regulation in type-1 helper T-cell inflammatory skin conditions, such as psoriasis vulgaris
- the interferon signaling pathway and the human glucocorticoid receptor gene 1A promoter interact in T-lymphocytes
- p27Kip1 inhibits hTERT mRNA expression and telomerase activity through post-transcriptional up-regulation by IFN-gamma/IRF-1 signaling
- TPO and IFN-gamma activate the expression of TAP1 via a new mechanism that involves functional cooperation between STAT1 and IRF-2 on the TAP1 promoter
- Functional role for IRF-1 in the growth suppression of breast cancer cells and strongly implicate IRF-1 as a tumor suppressor gene in breast cancer that act to control apoptosis.
- IRF-1 acts as a master regulator for the concerted expression of immunoproteasome components
- all-trans-retinoic acid, an RARalpha ligand, regulates IFNgamma-induced IRF-1 by affecting multiple components of the IFNgamma signaling pathway, from the plasma membrane to the nuclear transcription factors
- Individuals who were TT homozygous at IRF1 6477 T/G locus seemed to be attacked by Graves' disease much earlier than others.
- STAT1, IRF1, and NF-kappaB activation and early myocyte apoptosis play a mechanistic role in septic myocardial depression and sepsis-induced organ dysfunction
- The expression of IRF-1 truncated mRNA and protein forms might be a critical event in the development of Myelodysplastic Syndrome.
- Intracellular HIV-1 Tat protein represses constitutive LMP2 transcription increasing proteasome activity by interfering with the binding of IRF-1 to STAT1
- Data suggest that the dual effect of retinoids in increasing interferon regulatory factor-1 mRNA and nuclear protein may be essential for its tumor suppressor activity and immunosurveillance functions in breast epithelial cells.
- a novel repressor domain and differential gene regulation may contribute to IRF-1 tumor suppressor activity
- alternative splicing in exons 7, 8, and 9 is an important mechanism for negatively regulating IRF-1 in cervical cancer.
- findings suggest the possibility that the -300AA IRF-1 genotype is associated with outcome in patients with hepatitis C virus genotype 3 infection; in HCV genotype-1-infected patients, this genotype appears associated with response to therapy
- Increase in IRF-1 expression, induced by IFNgamma and arsenic, correlates with both higher anti-proliferative effect and increased apoptosis.
- no statistically significant association with susceptibility to persistent HBV infection was observed with the IFN-gamma, IFNGR-1 and 2, and IRF-1 gene polymorphisms under the codominant, dominant, and recessive models
- IRF-1 promotes rECM-mediated apoptosis and provide evidence that both rECM and rapid Tam signaling transcriptionally activate IRF-1 through recruitment of CBP to the IRF-1 GAS promoter complex.
- KPNA2 is physically bound to IFN regulatory factor-1 (IRF-1), a transcription factor induced by IFN-gamma, induces nuclear translocation of IRF-1 in dermal fibroblasts
- Results show A4396G single nucleotide polymorphism in IRF1 gene is more frequently expressed in African American than in European ancestry subjects.
- The A4396G single nucleotide polymorphism in IRF1 is more frequent in human breast cancer cell lines than in the general population and is more frequently expressed in African American than in Caucasian subjects.
- These results confirm that by controlling RIG-I expression, IRF-1 plays an essential role in anti-viral immunity.
- IFNalpha induces TRAIL expression via a STAT-1/IRF-1-dependent mechanism in human bladder cancer cells
- population-based data of IRF-1 sequence contains 35 nucleotide additions, 8 nucleotide removals and another 12 nucleotide replacements compared to GenBank sequence L05072; and a single nucleotide difference was observed in the IRF-1 promoter sequence
- IRF-1 is a susceptibility gene for Behcet's disease (BD) in Korean BD patients; its polymorphisms are associated with thrombotic manifestations in BD patients.
- EGFR induces expression of IRF-1 via STAT1 and STAT3 activation leading to growth arrest of human cancer cells
- These findings indicated that both the NF-kappaB and IRF-1 sites are required for the repression of promoter activity of IL-12 p40 by TGF-beta.
- These findings demonstrate for the first time that IRF-1 is a novel alternative GRbeta-independent mechanism mediating steroid dysfunction induced by pro-asthmatic cytokines.
- The described IRF-1 promoter single nucleotide polymorphisms do not play a role in the pathogenesis of psoriasis or in influencing IFN-alpha-induced Th1 polarization.
- IRF-1 activation regulates the induction of the IL-27p28 subunit by interferon beta.
- functionally relevant IRF-1 (interferon regulatory factor 1) polymorphisms influence atopy risk, potentially by altering transcription factor binding, IRF-1 gene expression, and IFN-gamma regulation.
- IRF-1 silencing through RNA interference, leads to an overexpression of TLR-4 in THP-1 cells.
- First evidence of the involvement in malaria susceptibility of a specific locus within the 5q31 region of interferon regulatory factor-1.
- These data indicate that in early phases of HIV-1 infection or during virus reactivation from latency, IRF-1 is an additional component of the p50/p65 heterodimer binding the LTR enhancer, absolutely required for efficient HIV-1 replication.
- Dendritic cell priming by L. major infection results in the early activation of NF-kappaB transcription factors and the up-regulation and nuclear translocation of interferon regulatory factor 1 (IRF-1) and IRF-8.
- data show that genetic variants in the IRF-1 and Stat1 genes of the IFN pathway are associated with multiple sclerosis and hepatitis c virus infection.
- KAP1 interacts with STAT1 and regulates IFN/STAT1-mediated IRF-1 gene expression in collaboration with HDACs.
- Although STAT1 phosphorylation required JNK and p38MAPK activation, only JNK activation was essential for IRF1 promoter activation by Tie2-R849W.
- involvement of IRF-1 in the regulation of SLC26A6 gene expression by IFNgamma in the human intestine
- TNF-alpha and PGE2 induce a complex that binds an oligonucleotide derived from the IRF1 promoter in dendritic cells.
- FOXA1 and IRF-1 intermediary transcriptional regulators of PPARgamma-induced urothelial cytodifferentiation
- These data reveal a novel "feed-forward" mechanism induced by NF-kappaB which ensures that acutely synthesized IRF-1 operates in concert with NF-kappaB to amplify the immunoproteasome and antigen-processing functions of CD40.
- NO inhibits HRV-16-induced production of CXCL10 by inhibiting viral activation of nuclear factor kappaB and of IRFs, including IRF-1, through a cGMP-independent pathway
- JNK1 stimulated and mediated the effects of IFN and TNF-alpha on XAF1 expression through transcriptional regulation by induction of IRF-1.