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Validated All-in-One™ qPCR Primer for AQP3(NM_004925.4) Search again
Product ID:
HQP009724
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
AQP-3, GIL
Gene Description:
aquaporin 3 (Gill blood group)
Target Gene Accession:
NM_004925.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Aquaporin 3 is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. [provided by RefSeq].
Gene References into function
- we analyzed the oligomerization of the AQP3 aquaglyceroporin, which presents a mixed selectivity for water, glycerol, and urea.
- the role of aquaporin-3 is to water-clamp viable layers of the epidermis in order to improve the hydration of the epidermis below the stratum corneum.
- Aquaporin-3 protein was detected in the human peritoneal tissue and is upregulated by glucose
- first reported cases of AQP3 deficiency in humans and provide the molecular basis of a new blood group system, GIL, encoded by the AQP3 protein
- analysis of effect of nickel and extracellular acidification on water permeability of human aquaporin-3 in lung epithelial cells
- reduction in the amounts of AQP-2 and AQP-3 expression, especially in lesions with substantial interstitial fibrosis and nephron loss, as compared with a healthy region of normal kidneys.
- results indicate that ANP and BNP up-regulated the expression of AQP3 mRNA and protein, and both PK-A and PK-G dependent pathways mediated this effect
- The distribution and expression levels of AQP3 in normal human tissue.
- analysis of AQP3 expression in human fetal airway epithelial progenitor cells
- AQP3 water channels serve as an essential pathway for volume-regulatory water transport in human epithelial cells.
- provides evidence of AQP3 expression in human fetal membranes and demonstrates that AQP3 expression in primary human amnion cell culture is up-regulated by second-messenger cAMP
- lower expression of AQP3 and enhanced expression of MRP2 are responsible for lower arsenic accumulation in arsenic-resistant cells
- Increased expression and altered cellular distribution of AQP3 is found in atopic eczema, and this may contribute to water loss.
- Lung carcinomas, especially adenocarcinomas, can produce AQP3, possibly in connection with their functional and/or biological nature.
- results suggest that Foxa2 is one of the transcriptional regulators for AQP3 gene expression regulated by insulin
- all trans retinoic acid increased AQP3 expression and enhanced biological activity in human skin
- Provide evidence for involvement of AQP3-facilitated water transport in epidermal cell migration and for AQP3-facilitated glycerol transport in epidermal cell proliferation.
- The results suggest that water-channel protein may be involved in the preservation of cellular character in the human prostate and prostate cancer is associated with an alteration of water-transporting mechanisms.
- The aim of this study was to use immunohistochemsitry to investigate the expression of aquaporins 1, 2 and 3 within the human intervertebral disc.
- The data suggest potential roles of AQP3 and 5 in the regulation of airway hypersecretion, perhaps ultimately providing a target for treating such diseases
- Although reticulocytes are highly abundant in malarial anemia, low AQP3 expression was observed
- Discuss the role of AQP3 in TNF-alpha-mediated inflammation and the development of periodontitis.
- The trafficking of AQP3 evoked by epinephrine is mediated by protein kinase C in Caco-2 cells.
- differential polarity and selective targeting of AQP3 and AQP10 in the intestinal epithelial cells is influenced by amino acid signal motifs.