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Validated All-in-One™ qPCR Primer for TNFRSF9(NM_001561.5) Search again
Product ID:
HQP009716
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
4-1BB, CD137, CDw137, ILA, IMD109
Gene Description:
TNF receptor superfamily member 9
Target Gene Accession:
NM_001561.5(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB. [provided by RefSeq].
Gene References into function
- intragraft gene expression is not a risk factor for acute cardiac allograft rejection
- 4-1BB enhanced expansion, survival and effector functions of newly primed CD8(+) T cells.
- 4-1BB plays a role in the differentiation of CD28(-)CD8(+) effector memory CTLs in cord blood.
- relative importance of CD134 (OX40) and CD137 (4-1BB) in the costimulation of CD4+ and CD8+ T cells under comparable conditions of antigenic stimulation
- 4-1 BB ligand can costimulate human CD28- T cells, resulting in cell division, inflammatory cytokine production, increased perforin levels, enhancement of cytolytic effector function, as well as the up-regulation of the anti-apoptotic protein Bcl-X(L).
- First evidence of expression and synthesis of CD137 and its ligand by human brain cells.
- 4-1BB mRNA, which was not detectable in normal liver, was found in 19 liver tissues adjacent to tumor edge (<1.0 cm). Low expression of 4-1BB mRNA was shown in hepatocellular carcinoma tissues and liver tissues within 1 to 5 cm away from tumor edge
- Data suggest that levels of soluble 4-1BB and 4-1BB ligand in sera at the time of diagnosis may be indicative of the severity and outcome of rheumatoid arthritis.
- 4-1BB/4-1BBL interactions contribute to the persistence of gut inflammation in Crohn's disease
- critical roles for interleukin-4 and IL-12 in regulating 4-1BB effects on Transforming growth factor-beta1-mediated suppression
- The CD137 is a member of the tumor necrosis factor receptor family and a potent regulator of T cell activities.
- The expression of CD134 was markedly higher, compared to CD137, both on the day of the surgery and ten days after colorectal cancer sursgery
- stimulation of OX40/4-1BB rendered cells sensitive to apoptosis induced by TNF-alpha and reduced activation of NF-kappaB. OX40/4-1BB stimulation repressed the mitogen response in activated CD25+CD4+ T cells and preactivated CD8+ T cells
- CD3+ T lymphocytes co-expressing CD134 and CD137 antigens on peripheral blood revealed an increased percentages of OX-40/CD137 positive cells in patients with Graves' disease (p<0.025) compared to the controls.
- Significantly higher soluble CD137 protein is associated with colorectal cancer patients
- Transduction of HMCLs with 4-1BBL retroviruses induced a high expression of 4-1BBL molecules and a strong T-cell activation ability.
- 4-1BB costimulation is essential for expanding memory CD8+ T cells ex vivo and is superior to CD28 costimulation for generating Ag-specific products for adoptive cell therapy
- showed CD137L to be a key costimulatory ligand for proliferation of CD28(-) CD45RA(hi) CD8(+) T cells and not CD80, CD86, or CD275 (ICOSL)
- Co-expression of NKG2D and 4-1BB may represent an important biomarker for defining competency of tumor infiltrating CD8(+) T cells
- Tax was sufficient to induce the expression of the endogenous 4-1BB gene in uninfected T cells, and it strongly activated (45-fold) the 4-1BB promoter via a single NF-kappaB site.
- CD137 can be enhanced on NK cells in an Fc-dependent fashion and expression correlates with phenotypic and functional parameters of activation.
- These data offer a novel approach for UCB Treg expansion using aAPCs, including those coexpressing OX40L or 4-1BBL.
- CCL23, M-CSF, TNFRSF9, TNF-alpha, and CXCL13 are predictive of rheumatoid arthritis disease activity and may be useful in the definition of disease subphenotypes and in the measurement of response to therapy in clinical studies.
- The majority of beryllium-responsive IFN-gamma-producing CD4+ T cells in blood coexpress CD28 and 4-1BB; a transition occurs within the memory CD4+ T cell population from CD28 dependence in blood to a requirement for 4-1BB costimulation in lung.
- Clinical association of serum CD137 (4-1BB) levels in patients with systemic sclerosis.