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Validated All-in-One™ qPCR Primer for IL12B(NM_002187.2) Search again
Product ID:
HQP009693
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CLMF, CLMF2, IL-12B, IMD28, IMD29, NKSF, NKSF2
Gene Description:
interleukin 12B
Target Gene Accession:
NM_002187.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. [provided by RefSeq].
Gene References into function
- inherited interleukin-12 deficiency: IL12B genotype and clinical phenotype of 13 patients from six kindreds
- Heparins (unfractionated heparin, UFH and low molecular weight heparin certoparin) had no effect on the release of IL-1ra, IL-10, and IL-12p40 from neutrophils or peripheral blood mononuclear cells.
- STATE OF ART REVIEW. Malignant B-cells from follicular and marginal zone lymphomas expressed IL-12 p35 and p40 transcripts, whereas only p35 mRNA was detected in mantle cell lymphoma.
- The IL12B gene does not confer susceptibility to coeliac disease
- results suggest that LPS stimulates the pituitary gland directly in vivo to increase IL-12 p40 gene expression
- DISCUSSION FORUM: it is the p40 protein, either alone or paired with other polypeptides, rather than p75, that acts as an interface between the innate and adaptive immune responses
- Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p40 secretion is strain and species dependent
- The purified lipoprotein, Lpk, from Mycobacterium leprae, was found to induce production of interleukin-12 in human peripheral blood monocytes
- results suggest that impaired IL-12 production in HIV-infected myeloid cells occurs, in part, via disruption of IL-12 p40 gene expression in a manner that requires cellular infection
- ATPgammaS potentiated the IL-12p40 release induced by TNF-alpha. This potentiation was observed as long as the IL-12p40 concentration under agonist stimulation remained below a threshold value close to 10 ng/ml; inhibition was observed above this value.
- IL12B promoter heterozygosity contributes to asthma severity rather than susceptibility per se.
- possible role of IL-12 and tissue antioxidants in development and progression of chronic sinusitis
- role of CD28 and IL-12/IL-15 signaling impairment in T cell proliferative deficiency during senescence
- IL12B SNP is associated with susceptibility to atopic dermatitis and psoriasis vulgaris, presumably by affecting the Th1/Th2 balance.
- IL-12 expression in PMN of SLE patients was less prominent than that of the normal controls.
- A key mechanism in the pathogenesis of MS is the increased expression of CD86 and CD40L and the increased production of IL12 during disease progression.
- Interleukin-10 induction of endothelial nitric-oxide synthase expression attenuates CD40-mediated interleukin-12 synthesis in human endothelial cells.
- Diesel exhaust particles seem to inhibit the production of IL-12 p40 in lipopolysaccharide activated monocytes as a model for lung macrophages
- These two potentially important candidate gene single nucleotide polymorphisms are not associated with susceptibility to idiopathic pulmonary fibrosis.
- IL-12B 3'untranslated region has no effect or has a negligible effect on human susceptibility to tuberculosis.
- The IL12B TaqI gene polymorphisms do not appear to be involved in susceptibility to celiac disease
- interleukin-12 p40 transcription and NF-kappaB activation are inhibited by nitric oxide in macrophages and dendritic cells
- Data show that reverse signaling via 4-1BB-ligand enhanced interleukin-12beta mRNA and the secretion of IL-12 p70 in various antigen-presenting cells, including monocytes.
- identify erythroid Kruppel-like factor as a transcription factor in macrophages able to regulate IL-12 p40 transcription depending on the cellular activation status
- no evidence to support the presence of a heterozygote effect of the IL12B promoter polymorphism on the level of asthma in early childhood or adulthood
- Frequencies of the various genotypes for the promoter region of the gene encoding IL-12p40 (IL12B) did not differ between psoriasis patients and controls.
- The polymorphism of IL12B (1188A/C) appears to have some influence on the outcome of hepatitis c virus infection.
- association between IL12B intron 2 polymorphism and tuberculosis and between specific IL12B haplotypes and TB found in Hong Kong Chinese population
- We found a strong association between the IL12B_4237 and IL12B_6402 polymorphisms and an asthma-severity phenotype in whites.
- Decreased LPS-induced IL-12(p70) and IL-10 responses were associated with the TLR4 (Asp299Gly) polymorphism and independently with asthma, especially atopic asthma in school-age children
- interleukin 12 and interferon gamma are involved in inhibition of cytotrophoblastic cell invasion
- No association of IL12B polymorphisms and self-limited HCV infection could be demonstrated.
- p300 is involved in the transcriptional regulation of IL-12 p40, and IL-12 p40 is one of the target genes of p300
- A direct role for IL12B in susceptibility to inflammatory bowel disease is unlikely.
- study shows that PBMCs of patients with advanced gastric cancer show poor production of TNF and IL-12p40 in response to stimulation with tumor cells in vitro & this unresponsiveness is associated in some patients with diminished IRAK-1 expression in vivo
- Heterozygosity of a polymorphism in the 3'untranslated region of the IL12B gene was associated with a delayed disease in late-onset diabetics.
- The IL12B 1188 (A-->C) gene polymorphism is not involved in defining the genetic basis of the susceptibility to and final outcome of peptic ulcer disease.
- synthesis in RAW264.7 cells is induced by IRF-8
- reversible histone acetylation/deacetylation modification plays an important role in the transcriptional regulation of IL-12
- chronically infected patients were significantly more likely than those with resolved HCV infection to be homozygous for the IL-12B 3'-UTR A allele
- IL12B gene polymorphisms may affect the final steps of gastric carcinogenesis in H pylori infected subjects.
- The IL-12B 3'UTR A-C polymorphism would be considered as a supplementary risk factor for Type 1 diabetes in conjunction with HLA haplotypes.
- The pro-inflammatory nature of hResistin was further evident from the ability of this protein to induce the nuclear translocation of NF-kappaB transcription factor as seen from electrophoretic mobility shift assays.
- Our results provide evidence for anti-bacterial host response toward Th1-immunity mediated by IL-12B in patients with Adamantiades-Behcet's disease (ABD), and the possible insight into the genetic susceptibility that is independent of HLA background.
- Among cytokines affecting T helper type 1 (Th1)-Th2 balance, the levels of IL-12 p40 and its autoantibody are specifically elevated in patients with myasthenia gravis complicated by thymoma.
- The 1188A/C exchange in the IL12B gene is associated with Th1-mediated infectious diseases such as tuberculosis and salmonellosis
- Increased concentrations of p40 subunit of IL-12/IL-23 in follicles containing oocytes suggest an important role of this cytokine in reproduction.
- The association of IL-12p40 A1188C polymorphism with the outcome of HCV infection is reported.
- IL12B and IL23R genes play a fundamental role in psoriasis pathogenesis.
- both CyA and FK506 down-regulated LPS-induced IL-12p40 transcription by inhibiting CaM/CaMK-II-activated PI3K and their downstream transcription factors NFkappaB and AP-1 independent of the JNK pathway.
- upon activation with anti-CD3 and anti-CD28 antibodies, only 25% of the CD4+ T cells from HIV-1 patients showed an increase in the IL-12beta2 when compared with 50% in healthy controls
- Polymorphic variants in the IL12B gene is associated with leprosy and tuberculosis
- IL-12B-3'UTR polymorphism may contribute to silicosis severity rather than to susceptibility
- IL-12p40-transgenic C57BL/6 mice characterized by overexpression of p40 show increased levels of serum IL-12p80 and enhanced disease susceptibility.
- mRNA levels of IL12 subunit p40 in active SLE patients was significantly higher compared with those in the inactive SLE patients
- downregulation of TLR2 and decreased production of interleukin-12 p40 and tumour necrosis factor-alpha following Mycobacterium avium or lipoteichoic acid stimulation may contribute to host susceptibility to nontuberculous mycobacterial lung disease
- IL-12 may have a role in progression of the colorectal adenoma-carcinoma sequence
- Sequence variants in the genes for the interleukin-23 receptor (IL23R) and its ligand (IL12B) confer protection against psoriasis.
- IL12B 3' UTR C allele was present at significantly higher frequency in cardiomyopathic as compared to asymptomatic. Results suggest that IL12B 3' UTR gene polymorphisms may influence the susceptibility to develop Chagasic cardiomyopathy.
- IL -10 (-1082 G/A) and IL -12B 3'UTR (Taq I) (A/C) gene polymorphisms were not associated either with susceptibility or resistance to pulmonary tuberculosis in the south Indian population.
- results here show that IL-23 is elevated in children with malarial anemia, and that IL-10 and IL-12 appear to have important regulatory effects on IL-23 production during childhood malaria
- polymorphism associated with early onset psoriasis
- A heightened, perhaps persistent, activational state of IL-12(p40)/Stat4 signaling may be present in active Chinese ulcerative colitis (UC) patients, and possibly involved in chronic inflammation in UC.
- no strong evidence was present in this study to support a role for the IL12B/IL23R psoriasis-associated SNPs in multiple sclerosis susceptibility; the potential role of other, unlinked SNPs in these genes with a very modest influence cannot be excluded
- Homozygosity of high producer IL-12p40 genotype IL-12Bpro-11 and -GG had the highest genetic risk of silicosis development in Bulgarian miners.
- Association of interleukin-6, interleukin-12, and interleukin-10 gene polymorphisms with essential hypertension in Tatars from Russia
- Polymorphisms of the IL12B and IL23R genes are associated with psoriasis.
- IL-12 p40 mRNA levels were significantly up-regulated in PHA-activated PBMCs.
- NK cells require in vivo priming with IL-12/23 to acquire their full spectrum of functional reactivity, while T cells are dependent upon IL-12/23 signals for the differentiation and/or the maintenance of CD56(+) effector memory T cells
- IL12B polymorphisms IVS2 -912GG genotype and IVS2 -912G:IVS4 +314A haplotype are associated with increased risk for cervical cancer in Korean women.
- Single nucleotide polymorphism in IL12B is associated with inflammatory bowel disease.
- The IL12B promoter polymorphism was associated with susceptibility to cerebral malaria.
- This study characterized the genetic variation in IL12B in 215 individuals belonging to 34 families and evaluated linkage and association of parasitemia with IL12B polymorphisms and haplotypes.
- IL-12 p40 3'UTR 1188 A/C polymorphism is associated with greater susceptibility to lepromatous leprosy in patients from western Mexico
- interleukin 12B (IL12B) variants are not associated with rheumatoid arthritis.
- These results implicate IL-12 and IL-27 in regulating human macrophages, and IL-27 derived from macrophages during infection impedes control of Mycobacterium tuberculosis growth.
- interleukin-12 has a role in the killing activity of lymphocytes on Hela cells
- Genotypical variations of the IL-12B (40) gene have not been investigated for schizophrenic patients yet.We examined polymorphic variants of IL-12B (p40).
- study demonstrates that oxidative stress induced by soluble cigarette smoke components potently inhibits the production of IL-12 and IL-23 by maturing dendritic cells
- results demonstrate that IL-12 improves the cytotoxicity of NK cells in an NKG2D-dependent manner and augments the expression of cytotoxic effector molecules
- Polymorphisms in the IL12B promoter region associate with two different clinical courses of steroid-sensitive childhood nephrotic syndrome.
- Data suggest that the structure of interleukin-23 reveals the molecular basis of p40 subunit sharing with interleukin-12.
- results demonstrated that the production of IL-10 from PBMC depended on both -1082A*G in IL10 and +16974A*C in IL12B polymorphisms
- IL-12 promotes disease development in acute inflammatory demyelinating polyradiculoneuropathy.
- variants are not specific risk factors for arthritis, but relevant for susceptibility to psoriasis in general
- in the presence of IL-18, cutaneous lupus erythematosus keratinocytes failed to express IL-12
- Variation within IL23R and IL12B is associated with susceptibility to both psoriasis and psoriatic arthritis
- Expanded catalog of genetic loci implicated in psoriasis susceptibility.
- Strong replication for two known susceptibility loci MHC and IL12B in psoriasis.