|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for CXCR2(NM_001557.3) Search again
Product ID:
HQP009681
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD182, CDw128b, CMKAR2, IL8R2, IL8RA, IL8RB, WHIMS2
Gene Description:
C-X-C motif chemokine receptor 2
Target Gene Accession:
NM_001557.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Summary
The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36.
Gene References into function
- Phagocytosing neutrophils down-regulate its expression
- CXCR1 and CXCR2 internalization and recycling are tightly regulated by receptor domains and by actin-related kinases.
- role in mediating angiogenic effects of interleukin effects in intestinal microvascular endothelial cells
- Neutrophil recruitment to inflammatory sites is mediated by two related receptors: CXCR1 and CXCR2. Both receptors share two ligands, interleukin-8 (CXCL8) and GCP-2 (CXCL6).
- functions as a dimer and truncated receptors negatively modulate receptor activities competing for the formation of wild type dimers
- a potentiation of Ins(1,4,5)P3 generation in the presence of coactivation of P2Y2 nucleotide receptors and CXCR2 would be sufficient for additional Ca2+ release.
- the data suggest that constitutive expression of CXCR1 and CXCR2 play an important role regulating the IL-8-mediated metastatic phenotype in human malignant melanoma cells.
- neutrophil migration in response to CXCR1 or CXCR2 agonists is not dependent on endocytosis of CXCR1 or CXCR2
- extra- and intracellular CXCR1 and CXCR2 are differentially expressed and regulated on T lymphocytes and mast cells
- The results support the hypothesis of bacterial propagation through CXCR2 neutrophil recruitment and confirm that tissue injury is unrelated to A. phagocytophilum tissue load.
- Arrestin has a role in regulating MAPK activation and preventing NADPH oxidase-dependent death of cells expressing CXCR2
- IL-8 and CXCR2 participate in the altered megakarocyte growth that features myeloid metaplasia with myelofibrosis
- membrane-bound activated Cdc42 and Rac1 localize to the leading edge of cells expressing wild-type CXCR2 receptor, but not in cells expressing mutant CXCR2
- Activation of neutrophils and down-regulation of CXCR2 were predominantly caused by IL-8
- In eutopic endometrium of women with endometriosis, significant increase in both proliferative and secretory phases for epithelial CXCR2 expression, and in proliferative phase for CXCR1 expression. May be involved in pathogenesis of endometriosis.
- neutrophils from transgenic mice were found to express hCXCR2 and to respond to CMV vCXCL-1
- the relative expression levels of CXCR-1 and -2 mRNA were rather lower than expected in the affected esophageal mucosa of patients with reflux esophagitis
- CXCR1-CXCR2 heterodimers are as likely to form in cells co-expressing these two chemokine receptors as the corresponding homodimers.
- We propose that the concurrence of CXCR2 on oligodendrocytes and induced CXCL1 on hypertrophic astrocytes in MS provides a novel mechanism for recruitment of oligodendrocytes to areas of damage, an essential prerequisite for lesion repair.
- Gene polymorphisms active in the EGFR pathway may be associated with the sensitivity of colorectal cancer patients to platinum-based chemotherapy.
- neutrophil migration induced by artocarpin involves binding to CXCR2
- increased expression of ELR+ CXC chemokines and their interaction with CXCR2 plays an important role in the pathogenesis of post-lung transplantation cold ischemia-reperfusion injury
- in CXCR2-expressing cells FAK phosphorylation was adhesion-dependent and was stimulated by fibronectin.Overall, several aspects of CXCL8-induced FAK phosphorylation and migration are regulated in a receptor-specific manner.
- No significant association existed between CXCR2 +1208 C/T polymorphism and multiple sclerosis susceptibility.
- CXCR-1 and CXCR-2 chemokine receptors of synovial fluid neutrophils may have diverse functions in the course of inflammatory arthritides
- analysis of the LLKIL motif in CXCR2, which is required for full IL-8 ligand-induced activation of Erk, Akt, and chemotaxis in HL60 cells
- TNF-alpha and IL-1beta enhance IL-8 expression in term decidual cells, suggesting that these cytokines are important regulators of chorioamnionitis-related decidual neutrophil infiltration.
- most circulating human CD4+ T cells store the inflammatory chemokine receptors CXCR3 and CXCR1 within a distinct intracellular compartment
- data herein indicate that the second extracellular loop (2ECL) of the receptors CXCR1,CXCR2, and CXCL8 is critical for the distinct rate of internalization of each
- CXCR2 receptor is important the homing of circulating endothelial progenitor cells.
- Mesenchymal stem cells express chemokine receptor CXCR2 and migrate upon stimulation with IL-8.
- By activating CXCR2, macrophage migration inhibitory factordisplays chemokine-like functions and acts as a major regulator of inflammatory cell recruitment and atherogenesis.
- Findings indicate that beta-endorphin and Met-enkephalin are contained in primary granules of PMN, and that CXCR1/2 ligands induce p38-dependent translocation and release of these opioid peptides to inhibit inflammatory pain
- The N-loop residues in IL-8 (H18 and F21) and the receptor N-termini are the major structural determinants regulating the rate of receptor internalization, which in turn controlls the activation profile of ERK1/2.
- CXCR2 +1208C/T polymorphism may affect the disease progression.
- Intracellular cross-talk between the GPCR CXCR1 and CXCR2: role of carboxyl terminus phosphorylation sites
- Cyr61 promotes interleukin-8-dependent chemotaxis, transendothelial migration, and intravasation by induction of CXCR1/CXCR2 through integrin alphavbeta3/Src/PI3K/Akt-dependent pathway.
- CXCR2 signaling promotes liver cyst growth in autosomal dominant polycystic kidney disease.
- Abnormal CXCR2 modulation and impaired expression cause systemic lupus erythematosis-neutrophil hyporesponsiveness to IL-8 stimulation in vitro.
- The allergen-induced levels of beta(c) mRNA and CCR3 mRNA in sputum-derived cells were inhibited by TPI ASM8, with no significant effects on the cell surface protein expression of CCR3 and beta(c).
- There is a novel mechanism via CXCR2 induction by PPAR-gamma that can enhance the immune response in human macrophages.
- Data show that CXCR2 chemokine receptor antagonism enhances delta opioid receptor (DOP) function via allosteric regulation of the CXCR2-DOP receptor heterodimer.
- Study reports that knocking down the chemokine receptor CXCR2 (IL8RB) alleviates both replicative and oncogene-induced senescence (OIS) and diminishes the DNA-damage response.
- the type I PDZ ligand of CXCR2 acts to both delay lysosomal sorting and facilitate proper chemotactic response.
- induction of autocrine CXCR2 signaling is a novel mode of resistance to oxaliplatin
- identify a pseudo-(E)LR motif as the structural determinant for MIF's activity as a non-canonical CXCR2 ligand
- No significant association was found between +2607 IL-8RA polymorphisms and nationality or in Behcets disease or health controls.
- PaCa-derived CXCL8 and fibroblast-derived CXCL12 and corresponding receptors CXCR2 and CXCR4 cooperatively induce angiogenesis in vitro by promoting HUVEC proliferation, invasion, and tube formation
- findings unravel new mechanisms involving the CXCR2 receptor in the pathogenesis of Alzheimer's disease and pose it as a potential target for developing novel therapeutics for intervention in this disease
- Studies show the importance of host CXCR2-dependent CXCL-8-mediated angiogenesis in the regulation of melanoma growth and metastasis.
- Data show that CXCL8 stimulation leads to paxillin phosphorylation in normal neutrophils, and that both CXCL8 receptors (CXCR1 and CXCR2) mediate CXCL8-induced paxillin phosphorylation.